Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_12775 and PS417_12780 are separated by 51 nucleotides PS417_12780 and PS417_12785 are separated by 184 nucleotides PS417_12785 and PS417_12790 are separated by 120 nucleotides
PS417_12775: PS417_12775 - GntR family transcriptional regulator, at 2,790,474 to 2,791,883
_12775
PS417_12780: PS417_12780 - (Fe-S)-binding protein, at 2,791,935 to 2,793,344
_12780
PS417_12785: PS417_12785 - hypothetical protein, at 2,793,529 to 2,793,768
_12785
PS417_12790: PS417_12790 - methyltransferase, at 2,793,889 to 2,794,380
_12790
Position (kb)
2791
2792
2793
2794 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 2791.033 kb on + strand, within PS417_12775 at 2791.072 kb on - strand, within PS417_12775 at 2791.072 kb on - strand, within PS417_12775 at 2791.072 kb on - strand, within PS417_12775 at 2791.532 kb on + strand, within PS417_12775 at 2791.533 kb on - strand, within PS417_12775 at 2791.741 kb on + strand, within PS417_12775 at 2791.741 kb on + strand at 2791.741 kb on + strand, within PS417_12775 at 2791.742 kb on - strand, within PS417_12775 at 2791.744 kb on + strand at 2791.744 kb on + strand at 2791.745 kb on - strand at 2791.745 kb on - strand at 2791.777 kb on + strand at 2791.777 kb on + strand at 2791.919 kb on + strand at 2791.978 kb on + strand at 2791.978 kb on + strand at 2792.070 kb on + strand at 2792.070 kb on + strand at 2792.070 kb on + strand at 2792.070 kb on + strand at 2792.071 kb on - strand at 2792.197 kb on + strand, within PS417_12780 at 2792.198 kb on - strand, within PS417_12780 at 2792.392 kb on + strand, within PS417_12780 at 2792.650 kb on - strand, within PS417_12780 at 2792.650 kb on - strand, within PS417_12780 at 2792.719 kb on + strand, within PS417_12780 at 2792.719 kb on + strand, within PS417_12780 at 2792.720 kb on - strand, within PS417_12780 at 2792.720 kb on - strand, within PS417_12780 at 2793.231 kb on - strand at 2793.258 kb on - strand at 2793.382 kb on + strand at 2793.383 kb on - strand at 2793.383 kb on - strand at 2793.624 kb on - strand, within PS417_12785 at 2793.624 kb on - strand, within PS417_12785 at 2793.624 kb on - strand at 2793.624 kb on - strand, within PS417_12785 at 2793.681 kb on + strand, within PS417_12785 at 2793.737 kb on + strand, within PS417_12785 at 2793.737 kb on + strand, within PS417_12785 at 2793.737 kb on + strand, within PS417_12785 at 2793.738 kb on - strand, within PS417_12785 at 2793.738 kb on - strand, within PS417_12785 at 2793.738 kb on - strand, within PS417_12785 at 2794.314 kb on + strand, within PS417_12790
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 2,791,033 + PS417_12775 0.40 +0.7 2,791,072 - PS417_12775 0.42 +0.3 2,791,072 - PS417_12775 0.42 -1.5 2,791,072 - PS417_12775 0.42 -2.5 2,791,532 + PS417_12775 0.75 +1.9 2,791,533 - PS417_12775 0.75 -0.9 2,791,741 + PS417_12775 0.90 +2.1 2,791,741 + -0.1 2,791,741 + PS417_12775 0.90 +0.6 2,791,742 - PS417_12775 0.90 -2.4 2,791,744 + +1.3 2,791,744 + -1.0 2,791,745 - +0.6 2,791,745 - +0.9 2,791,777 + -0.1 2,791,777 + +0.7 2,791,919 + +0.7 2,791,978 + -0.3 2,791,978 + +0.1 2,792,070 + +0.6 2,792,070 + -1.3 2,792,070 + +0.1 2,792,070 + +0.6 2,792,071 - -0.1 2,792,197 + PS417_12780 0.19 +0.1 2,792,198 - PS417_12780 0.19 -1.6 2,792,392 + PS417_12780 0.32 -1.5 2,792,650 - PS417_12780 0.51 +5.1 2,792,650 - PS417_12780 0.51 -1.1 2,792,719 + PS417_12780 0.56 +1.4 2,792,719 + PS417_12780 0.56 +1.7 2,792,720 - PS417_12780 0.56 -2.1 2,792,720 - PS417_12780 0.56 -0.7 2,793,231 - -2.2 2,793,258 - -0.1 2,793,382 + +0.9 2,793,383 - -1.4 2,793,383 - +0.9 2,793,624 - PS417_12785 0.40 -3.4 2,793,624 - PS417_12785 0.40 -0.7 2,793,624 - -1.1 2,793,624 - PS417_12785 0.40 +0.2 2,793,681 + PS417_12785 0.63 -0.8 2,793,737 + PS417_12785 0.87 +0.7 2,793,737 + PS417_12785 0.87 +0.3 2,793,737 + PS417_12785 0.87 +0.2 2,793,738 - PS417_12785 0.87 +1.2 2,793,738 - PS417_12785 0.87 +0.5 2,793,738 - PS417_12785 0.87 +1.2 2,794,314 + PS417_12790 0.86 +1.0
Or see this region's nucleotide sequence