Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_12645 and PS417_12650 are separated by 7 nucleotides
PS417_12645: PS417_12645 - carboxylate--amine ligase, at 2,752,074 to 2,753,660
_12645
PS417_12650: PS417_12650 - magnesium transporter, at 2,753,668 to 2,754,606
_12650
Position (kb)
2753
2754
2755 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2753.034 kb on + strand at 2753.035 kb on - strand, within PS417_12645 at 2753.035 kb on - strand, within PS417_12645 at 2753.194 kb on + strand, within PS417_12645 at 2753.195 kb on - strand, within PS417_12645 at 2753.283 kb on + strand, within PS417_12645 at 2753.284 kb on - strand, within PS417_12645 at 2753.284 kb on - strand, within PS417_12645 at 2753.339 kb on + strand, within PS417_12645 at 2753.340 kb on - strand, within PS417_12645 at 2753.340 kb on - strand, within PS417_12645 at 2753.340 kb on - strand, within PS417_12645 at 2753.364 kb on + strand, within PS417_12645 at 2753.364 kb on + strand, within PS417_12645 at 2753.365 kb on - strand, within PS417_12645 at 2753.365 kb on - strand, within PS417_12645 at 2753.487 kb on + strand, within PS417_12645 at 2753.578 kb on - strand at 2753.578 kb on - strand at 2753.596 kb on - strand at 2753.704 kb on + strand at 2753.788 kb on - strand, within PS417_12650 at 2754.188 kb on - strand, within PS417_12650 at 2754.188 kb on - strand, within PS417_12650 at 2754.190 kb on + strand, within PS417_12650 at 2754.191 kb on - strand, within PS417_12650 at 2754.316 kb on + strand at 2754.634 kb on - strand at 2754.695 kb on - strand at 2754.695 kb on - strand at 2754.802 kb on - strand at 2754.802 kb on - strand at 2755.309 kb on + strand at 2755.479 kb on + strand at 2755.480 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 2,753,034 + +0.8 2,753,035 - PS417_12645 0.61 +1.8 2,753,035 - PS417_12645 0.61 -2.6 2,753,194 + PS417_12645 0.71 +1.7 2,753,195 - PS417_12645 0.71 -0.5 2,753,283 + PS417_12645 0.76 -0.8 2,753,284 - PS417_12645 0.76 -0.7 2,753,284 - PS417_12645 0.76 -1.4 2,753,339 + PS417_12645 0.80 +0.9 2,753,340 - PS417_12645 0.80 -0.4 2,753,340 - PS417_12645 0.80 +0.7 2,753,340 - PS417_12645 0.80 -0.2 2,753,364 + PS417_12645 0.81 -3.8 2,753,364 + PS417_12645 0.81 -0.9 2,753,365 - PS417_12645 0.81 -0.9 2,753,365 - PS417_12645 0.81 -1.6 2,753,487 + PS417_12645 0.89 -0.5 2,753,578 - +0.5 2,753,578 - +0.2 2,753,596 - -0.5 2,753,704 + -2.3 2,753,788 - PS417_12650 0.13 -0.4 2,754,188 - PS417_12650 0.55 +0.3 2,754,188 - PS417_12650 0.55 -1.1 2,754,190 + PS417_12650 0.56 -0.7 2,754,191 - PS417_12650 0.56 +2.1 2,754,316 + -0.4 2,754,634 - -0.3 2,754,695 - +2.5 2,754,695 - +1.6 2,754,802 - -0.1 2,754,802 - +0.4 2,755,309 + -0.9 2,755,479 + -2.4 2,755,480 - -0.0
Or see this region's nucleotide sequence