Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_12585 and PS417_12590 are separated by 6 nucleotides PS417_12590 and PS417_12595 are separated by 287 nucleotides PS417_12595 and PS417_12600 are separated by 3 nucleotides
PS417_12585: PS417_12585 - acriflavine resistance protein B, at 2,737,934 to 2,741,026
_12585
PS417_12590: PS417_12590 - RND transporter, at 2,741,033 to 2,742,175
_12590
PS417_12595: PS417_12595 - transcriptional regulator, at 2,742,463 to 2,743,164
_12595
PS417_12600: PS417_12600 - histidine kinase, at 2,743,168 to 2,744,469
_12600
Position (kb)
2741
2742
2743 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 2740.063 kb on - strand, within PS417_12585 at 2740.158 kb on - strand, within PS417_12585 at 2740.206 kb on + strand, within PS417_12585 at 2740.497 kb on + strand, within PS417_12585 at 2740.798 kb on - strand at 2740.882 kb on - strand at 2740.884 kb on - strand at 2740.890 kb on + strand at 2741.012 kb on - strand at 2742.276 kb on + strand at 2742.276 kb on + strand at 2742.277 kb on - strand at 2742.292 kb on + strand at 2742.292 kb on + strand at 2742.293 kb on - strand at 2742.293 kb on - strand at 2742.293 kb on - strand at 2742.293 kb on - strand at 2742.295 kb on - strand at 2742.361 kb on + strand at 2742.757 kb on + strand, within PS417_12595 at 2742.940 kb on + strand, within PS417_12595 at 2743.132 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 2,740,063 - PS417_12585 0.69 -3.3 2,740,158 - PS417_12585 0.72 -1.0 2,740,206 + PS417_12585 0.73 +0.2 2,740,497 + PS417_12585 0.83 -3.4 2,740,798 - -3.5 2,740,882 - -4.3 2,740,884 - -3.1 2,740,890 + -2.8 2,741,012 - -1.5 2,742,276 + +0.6 2,742,276 + +1.4 2,742,277 - +2.1 2,742,292 + -0.1 2,742,292 + -0.9 2,742,293 - +0.6 2,742,293 - -1.7 2,742,293 - -3.1 2,742,293 - -0.5 2,742,295 - +1.1 2,742,361 + +1.6 2,742,757 + PS417_12595 0.42 +2.0 2,742,940 + PS417_12595 0.68 +2.7 2,743,132 + +4.3
Or see this region's nucleotide sequence