Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_12435 and PS417_12445 are separated by 611 nucleotides PS417_12445 and PS417_12450 overlap by 4 nucleotides
PS417_12435: PS417_12435 - hypothetical protein, at 2,704,450 to 2,706,201
_12435
PS417_12445: PS417_12445 - 6-phosphogluconate dehydrogenase, at 2,706,813 to 2,707,796
_12445
PS417_12450: PS417_12450 - glucose-6-phosphate dehydrogenase, at 2,707,793 to 2,709,316
_12450
Position (kb)
2706
2707
2708 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2705.921 kb on + strand, within PS417_12435 at 2706.119 kb on + strand at 2706.177 kb on + strand at 2706.177 kb on + strand at 2706.178 kb on - strand at 2706.306 kb on + strand at 2706.306 kb on + strand at 2706.306 kb on + strand at 2706.344 kb on + strand at 2706.502 kb on + strand at 2706.502 kb on + strand at 2706.502 kb on + strand at 2706.502 kb on + strand at 2706.503 kb on - strand at 2706.503 kb on - strand at 2706.503 kb on - strand at 2706.547 kb on + strand at 2706.548 kb on - strand at 2706.565 kb on + strand at 2706.619 kb on + strand at 2706.768 kb on + strand at 2706.775 kb on + strand at 2706.775 kb on + strand at 2706.775 kb on + strand at 2706.776 kb on - strand at 2706.776 kb on - strand at 2706.791 kb on + strand at 2706.794 kb on + strand at 2706.795 kb on - strand at 2706.798 kb on + strand at 2706.798 kb on + strand at 2706.855 kb on - strand at 2706.884 kb on + strand at 2706.885 kb on - strand at 2706.919 kb on + strand at 2707.000 kb on + strand, within PS417_12445 at 2707.104 kb on + strand, within PS417_12445 at 2707.105 kb on - strand, within PS417_12445 at 2707.106 kb on + strand, within PS417_12445 at 2707.155 kb on + strand, within PS417_12445 at 2707.253 kb on + strand, within PS417_12445 at 2707.253 kb on + strand, within PS417_12445 at 2707.254 kb on - strand, within PS417_12445 at 2707.341 kb on + strand, within PS417_12445 at 2707.341 kb on + strand, within PS417_12445 at 2707.342 kb on - strand, within PS417_12445 at 2707.728 kb on + strand at 2707.728 kb on + strand at 2707.729 kb on - strand at 2707.902 kb on - strand at 2707.902 kb on - strand at 2707.943 kb on - strand at 2708.129 kb on + strand, within PS417_12450 at 2708.130 kb on - strand, within PS417_12450 at 2708.130 kb on - strand, within PS417_12450 at 2708.130 kb on - strand, within PS417_12450 at 2708.130 kb on - strand, within PS417_12450 at 2708.130 kb on - strand, within PS417_12450 at 2708.130 kb on - strand, within PS417_12450 at 2708.462 kb on + strand, within PS417_12450 at 2708.462 kb on + strand, within PS417_12450 at 2708.463 kb on - strand, within PS417_12450 at 2708.463 kb on - strand, within PS417_12450
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 2,705,921 + PS417_12435 0.84 +0.0 2,706,119 + +0.3 2,706,177 + -1.0 2,706,177 + -3.1 2,706,178 - -1.8 2,706,306 + -3.1 2,706,306 + -1.8 2,706,306 + -0.4 2,706,344 + -0.3 2,706,502 + -2.5 2,706,502 + -0.1 2,706,502 + +0.2 2,706,502 + -1.0 2,706,503 - +1.4 2,706,503 - +0.6 2,706,503 - -0.4 2,706,547 + +0.2 2,706,548 - +0.5 2,706,565 + -0.5 2,706,619 + -2.1 2,706,768 + +0.5 2,706,775 + -0.1 2,706,775 + +2.4 2,706,775 + -0.8 2,706,776 - +1.4 2,706,776 - +2.4 2,706,791 + -1.5 2,706,794 + +0.8 2,706,795 - +1.2 2,706,798 + -1.0 2,706,798 + +0.0 2,706,855 - -0.7 2,706,884 + +2.4 2,706,885 - +0.9 2,706,919 + +1.6 2,707,000 + PS417_12445 0.19 +2.2 2,707,104 + PS417_12445 0.30 -0.5 2,707,105 - PS417_12445 0.30 -1.4 2,707,106 + PS417_12445 0.30 +2.2 2,707,155 + PS417_12445 0.35 +3.7 2,707,253 + PS417_12445 0.45 +0.5 2,707,253 + PS417_12445 0.45 +0.8 2,707,254 - PS417_12445 0.45 -2.9 2,707,341 + PS417_12445 0.54 -0.8 2,707,341 + PS417_12445 0.54 +0.0 2,707,342 - PS417_12445 0.54 -1.7 2,707,728 + +0.3 2,707,728 + -3.1 2,707,729 - -2.4 2,707,902 - +1.4 2,707,902 - +0.5 2,707,943 - -0.3 2,708,129 + PS417_12450 0.22 -1.6 2,708,130 - PS417_12450 0.22 +1.9 2,708,130 - PS417_12450 0.22 +1.0 2,708,130 - PS417_12450 0.22 +0.3 2,708,130 - PS417_12450 0.22 +1.1 2,708,130 - PS417_12450 0.22 +0.8 2,708,130 - PS417_12450 0.22 +1.6 2,708,462 + PS417_12450 0.44 +2.8 2,708,462 + PS417_12450 0.44 -1.0 2,708,463 - PS417_12450 0.44 +1.2 2,708,463 - PS417_12450 0.44 +0.8
Or see this region's nucleotide sequence