Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_12270 and PS417_12275 are separated by 92 nucleotides PS417_12275 and PS417_12280 are separated by 71 nucleotides PS417_12280 and PS417_12285 are separated by 97 nucleotides
PS417_12270: PS417_12270 - pyruvate dehydrogenase, at 2,665,309 to 2,667,018
_12270
PS417_12275: PS417_12275 - 2,4-dienoyl-CoA reductase, at 2,667,111 to 2,668,343
_12275
PS417_12280: PS417_12280 - MerR family transcriptional regulator, at 2,668,415 to 2,668,819
_12280
PS417_12285: PS417_12285 - oxidoreductase, at 2,668,917 to 2,669,414
_12285
Position (kb)
2667
2668
2669 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2666.341 kb on + strand, within PS417_12270 at 2667.181 kb on - strand at 2667.191 kb on + strand at 2667.411 kb on + strand, within PS417_12275 at 2667.412 kb on - strand, within PS417_12275 at 2667.505 kb on + strand, within PS417_12275 at 2667.506 kb on - strand, within PS417_12275 at 2667.824 kb on - strand, within PS417_12275 at 2668.216 kb on + strand, within PS417_12275 at 2668.216 kb on + strand, within PS417_12275 at 2668.217 kb on - strand, within PS417_12275 at 2668.217 kb on - strand, within PS417_12275 at 2668.217 kb on - strand, within PS417_12275 at 2668.289 kb on + strand at 2668.377 kb on - strand at 2668.382 kb on + strand at 2668.395 kb on - strand at 2668.472 kb on + strand, within PS417_12280 at 2668.529 kb on + strand, within PS417_12280 at 2668.791 kb on + strand at 2668.791 kb on + strand at 2668.966 kb on + strand at 2668.966 kb on + strand at 2668.966 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 2,666,341 + PS417_12270 0.60 -0.5 2,667,181 - -0.1 2,667,191 + +0.8 2,667,411 + PS417_12275 0.24 -2.1 2,667,412 - PS417_12275 0.24 -0.7 2,667,505 + PS417_12275 0.32 -2.3 2,667,506 - PS417_12275 0.32 +0.8 2,667,824 - PS417_12275 0.58 -0.0 2,668,216 + PS417_12275 0.90 -0.7 2,668,216 + PS417_12275 0.90 +0.3 2,668,217 - PS417_12275 0.90 -0.9 2,668,217 - PS417_12275 0.90 -3.0 2,668,217 - PS417_12275 0.90 -0.5 2,668,289 + -1.2 2,668,377 - +1.3 2,668,382 + -2.4 2,668,395 - +0.7 2,668,472 + PS417_12280 0.14 +1.6 2,668,529 + PS417_12280 0.28 +0.1 2,668,791 + -0.7 2,668,791 + -1.0 2,668,966 + -1.3 2,668,966 + -0.1 2,668,966 + +1.2
Or see this region's nucleotide sequence