Strain Fitness in Pseudomonas simiae WCS417 around PS417_00125

Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPS417_00120 and PS417_00125 overlap by 1 nucleotidesPS417_00125 and PS417_00130 are separated by 36 nucleotides PS417_00120: PS417_00120 - coproporphyrinogen III oxidase, at 25,508 to 26,440 _00120 PS417_00125: PS417_00125 - shikimate dehydrogenase, at 26,440 to 27,258 _00125 PS417_00130: PS417_00130 - SulP family inorganic anion transporter, at 27,295 to 28,887 _00130 Position (kb) 26 27 28Strain fitness (log2 ratio) -1 0 1 2at 26.536 kb on + strand, within PS417_00125at 27.190 kb on + strandat 27.263 kb on - strandat 27.263 kb on - strandat 27.468 kb on - strand, within PS417_00130at 27.468 kb on - strand, within PS417_00130at 27.542 kb on + strand, within PS417_00130at 27.542 kb on + strand, within PS417_00130

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml
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26,536 + PS417_00125 0.12 +0.2
27,190 + +0.8
27,263 - +1.0
27,263 - +1.5
27,468 - PS417_00130 0.11 +1.8
27,468 - PS417_00130 0.11 -0.5
27,542 + PS417_00130 0.16 +0.9
27,542 + PS417_00130 0.16 +0.6

Or see this region's nucleotide sequence