Strain Fitness in Pseudomonas simiae WCS417 around PS417_01200

Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPS417_01190 and PS417_01195 are separated by 115 nucleotidesPS417_01195 and PS417_01200 are separated by 39 nucleotidesPS417_01200 and PS417_01205 are separated by 104 nucleotides PS417_01190: PS417_01190 - diguanylate phosphodiesterase, at 262,104 to 262,883 _01190 PS417_01195: PS417_01195 - RNA-binding protein S4, at 262,999 to 263,406 _01195 PS417_01200: PS417_01200 - phosphoesterase, at 263,446 to 264,246 _01200 PS417_01205: PS417_01205 - molecular chaperone Hsp33, at 264,351 to 265,253 _01205 Position (kb) 263 264 265Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 262.465 kb on + strand, within PS417_01190at 262.469 kb on + strand, within PS417_01190at 262.469 kb on + strand, within PS417_01190at 262.469 kb on + strand, within PS417_01190at 262.469 kb on + strand, within PS417_01190at 262.469 kb on + strand, within PS417_01190at 262.469 kb on + strand, within PS417_01190at 262.469 kb on + strand, within PS417_01190at 262.469 kb on + strand, within PS417_01190at 262.469 kb on + strand, within PS417_01190at 262.470 kb on - strand, within PS417_01190at 262.473 kb on + strand, within PS417_01190at 262.473 kb on + strand, within PS417_01190at 262.474 kb on - strand, within PS417_01190at 262.474 kb on - strand, within PS417_01190at 262.474 kb on - strand, within PS417_01190at 262.474 kb on - strand, within PS417_01190at 262.523 kb on + strand, within PS417_01190at 262.523 kb on + strand, within PS417_01190at 262.523 kb on + strand, within PS417_01190at 262.523 kb on + strand, within PS417_01190at 262.523 kb on + strand, within PS417_01190at 262.524 kb on - strand, within PS417_01190at 262.575 kb on + strand, within PS417_01190at 262.575 kb on + strand, within PS417_01190at 262.576 kb on - strand, within PS417_01190at 262.649 kb on + strand, within PS417_01190at 262.701 kb on + strand, within PS417_01190at 262.888 kb on + strandat 262.889 kb on - strandat 262.909 kb on + strandat 262.909 kb on + strandat 262.909 kb on + strandat 262.910 kb on - strandat 262.923 kb on + strandat 262.923 kb on + strandat 262.924 kb on - strandat 263.065 kb on + strand, within PS417_01195at 263.066 kb on - strandat 263.257 kb on + strand, within PS417_01195at 263.257 kb on + strand, within PS417_01195at 263.539 kb on + strand, within PS417_01200at 263.561 kb on + strand, within PS417_01200at 263.561 kb on + strand, within PS417_01200at 263.562 kb on - strand, within PS417_01200at 263.562 kb on - strand, within PS417_01200at 263.609 kb on + strand, within PS417_01200at 263.609 kb on + strand, within PS417_01200at 263.609 kb on + strand, within PS417_01200at 263.859 kb on + strand, within PS417_01200at 263.877 kb on + strand, within PS417_01200at 263.878 kb on - strand, within PS417_01200at 263.937 kb on + strand, within PS417_01200at 263.944 kb on + strand, within PS417_01200at 263.945 kb on - strand, within PS417_01200at 264.058 kb on + strand, within PS417_01200at 264.184 kb on + strandat 264.184 kb on + strandat 264.246 kb on + strandat 264.247 kb on - strandat 264.316 kb on + strandat 264.316 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml
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262,465 + PS417_01190 0.46 -0.3
262,469 + PS417_01190 0.47 -1.8
262,469 + PS417_01190 0.47 -0.8
262,469 + PS417_01190 0.47 +1.1
262,469 + PS417_01190 0.47 +1.4
262,469 + PS417_01190 0.47 +0.5
262,469 + PS417_01190 0.47 +1.2
262,469 + PS417_01190 0.47 +1.7
262,469 + PS417_01190 0.47 +0.8
262,469 + PS417_01190 0.47 -0.2
262,470 - PS417_01190 0.47 -0.4
262,473 + PS417_01190 0.47 -0.1
262,473 + PS417_01190 0.47 +0.1
262,474 - PS417_01190 0.47 -1.7
262,474 - PS417_01190 0.47 -2.2
262,474 - PS417_01190 0.47 +0.5
262,474 - PS417_01190 0.47 -0.3
262,523 + PS417_01190 0.54 +0.3
262,523 + PS417_01190 0.54 +0.7
262,523 + PS417_01190 0.54 -2.3
262,523 + PS417_01190 0.54 +1.6
262,523 + PS417_01190 0.54 +1.8
262,524 - PS417_01190 0.54 -2.3
262,575 + PS417_01190 0.60 +1.0
262,575 + PS417_01190 0.60 +0.2
262,576 - PS417_01190 0.61 -0.0
262,649 + PS417_01190 0.70 +1.3
262,701 + PS417_01190 0.77 -2.5
262,888 + +0.4
262,889 - -2.7
262,909 + +1.8
262,909 + +1.6
262,909 + +0.7
262,910 - -1.2
262,923 + -0.2
262,923 + +0.6
262,924 - -2.0
263,065 + PS417_01195 0.16 -1.4
263,066 - -0.2
263,257 + PS417_01195 0.63 -0.9
263,257 + PS417_01195 0.63 +1.0
263,539 + PS417_01200 0.12 +2.5
263,561 + PS417_01200 0.14 +2.3
263,561 + PS417_01200 0.14 +0.4
263,562 - PS417_01200 0.14 +3.4
263,562 - PS417_01200 0.14 +0.8
263,609 + PS417_01200 0.20 +1.6
263,609 + PS417_01200 0.20 +1.6
263,609 + PS417_01200 0.20 +3.1
263,859 + PS417_01200 0.52 +1.7
263,877 + PS417_01200 0.54 +3.7
263,878 - PS417_01200 0.54 +0.4
263,937 + PS417_01200 0.61 +2.5
263,944 + PS417_01200 0.62 +2.8
263,945 - PS417_01200 0.62 +2.5
264,058 + PS417_01200 0.76 -0.3
264,184 + +4.1
264,184 + +2.8
264,246 + +1.9
264,247 - +3.4
264,316 + +1.9
264,316 + +3.0

Or see this region's nucleotide sequence