Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_00115 and PS417_00120 are separated by 159 nucleotides PS417_00120 and PS417_00125 overlap by 1 nucleotides PS417_00125 and PS417_00130 are separated by 36 nucleotides
PS417_00115: PS417_00115 - quinone oxidoreductase, at 24,371 to 25,348
_00115
PS417_00120: PS417_00120 - coproporphyrinogen III oxidase, at 25,508 to 26,440
_00120
PS417_00125: PS417_00125 - shikimate dehydrogenase, at 26,440 to 27,258
_00125
PS417_00130: PS417_00130 - SulP family inorganic anion transporter, at 27,295 to 28,887
_00130
Position (kb) 25
26
27 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 24.526 kb on + strand, within PS417_00115 at 24.526 kb on + strand, within PS417_00115 at 24.527 kb on - strand, within PS417_00115 at 24.527 kb on - strand, within PS417_00115 at 24.543 kb on + strand, within PS417_00115 at 24.570 kb on + strand, within PS417_00115 at 24.571 kb on - strand, within PS417_00115 at 24.709 kb on - strand, within PS417_00115 at 24.942 kb on + strand, within PS417_00115 at 24.942 kb on + strand, within PS417_00115 at 24.942 kb on + strand, within PS417_00115 at 24.943 kb on - strand, within PS417_00115 at 24.943 kb on - strand, within PS417_00115 at 25.065 kb on + strand, within PS417_00115 at 25.212 kb on + strand, within PS417_00115 at 25.213 kb on - strand, within PS417_00115 at 25.247 kb on - strand, within PS417_00115 at 25.287 kb on + strand at 25.287 kb on + strand at 25.287 kb on + strand at 25.288 kb on - strand at 25.288 kb on - strand at 25.296 kb on + strand at 25.296 kb on + strand at 25.296 kb on + strand at 25.297 kb on - strand at 25.395 kb on + strand at 25.395 kb on + strand at 25.396 kb on - strand at 25.396 kb on - strand at 25.438 kb on + strand at 26.536 kb on + strand, within PS417_00125 at 27.190 kb on + strand at 27.263 kb on - strand at 27.263 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 24,526 + PS417_00115 0.16 +2.6 24,526 + PS417_00115 0.16 -2.0 24,527 - PS417_00115 0.16 -2.0 24,527 - PS417_00115 0.16 +2.4 24,543 + PS417_00115 0.18 +0.8 24,570 + PS417_00115 0.20 +0.1 24,571 - PS417_00115 0.20 +0.5 24,709 - PS417_00115 0.35 -1.1 24,942 + PS417_00115 0.58 +1.7 24,942 + PS417_00115 0.58 -0.4 24,942 + PS417_00115 0.58 +3.0 24,943 - PS417_00115 0.58 +1.8 24,943 - PS417_00115 0.58 +1.2 25,065 + PS417_00115 0.71 +1.2 25,212 + PS417_00115 0.86 +2.1 25,213 - PS417_00115 0.86 +0.4 25,247 - PS417_00115 0.90 -1.1 25,287 + -1.6 25,287 + -0.1 25,287 + +0.2 25,288 - +0.7 25,288 - -1.2 25,296 + -0.5 25,296 + +1.8 25,296 + +0.8 25,297 - +0.1 25,395 + -0.3 25,395 + -0.6 25,396 - +0.8 25,396 - -2.9 25,438 + +2.1 26,536 + PS417_00125 0.12 +0.2 27,190 + +0.8 27,263 - +1.0 27,263 - +1.5
Or see this region's nucleotide sequence