Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_11535 and PS417_11540 are separated by 1 nucleotides PS417_11540 and PS417_11545 are separated by 34 nucleotides PS417_11545 and PS417_11550 are separated by 14 nucleotides
PS417_11535: PS417_11535 - nicotinate phosphoribosyltransferase, at 2,480,035 to 2,481,252
_11535
PS417_11540: PS417_11540 - NAD synthetase, at 2,481,254 to 2,482,063
_11540
PS417_11545: PS417_11545 - spermidine/putrescine ABC transporter substrate-binding protein, at 2,482,098 to 2,483,129
_11545
PS417_11550: PS417_11550 - FAD-binding oxidoreductase, at 2,483,144 to 2,484,241
_11550
Position (kb)
2482
2483
2484 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2481.211 kb on + strand at 2481.212 kb on - strand at 2481.926 kb on + strand, within PS417_11540 at 2481.926 kb on + strand, within PS417_11540 at 2481.926 kb on + strand, within PS417_11540 at 2481.927 kb on - strand, within PS417_11540 at 2481.932 kb on + strand, within PS417_11540 at 2481.933 kb on - strand, within PS417_11540 at 2481.945 kb on - strand, within PS417_11540 at 2482.061 kb on + strand at 2482.061 kb on + strand at 2482.402 kb on + strand, within PS417_11545 at 2482.741 kb on + strand, within PS417_11545 at 2482.742 kb on - strand, within PS417_11545 at 2482.867 kb on + strand, within PS417_11545 at 2483.117 kb on + strand at 2483.511 kb on + strand, within PS417_11550 at 2483.511 kb on + strand, within PS417_11550 at 2483.599 kb on + strand, within PS417_11550 at 2484.003 kb on + strand, within PS417_11550 at 2484.071 kb on + strand at 2484.071 kb on + strand, within PS417_11550 at 2484.072 kb on - strand, within PS417_11550 at 2484.111 kb on + strand, within PS417_11550
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 2,481,211 + +0.0 2,481,212 - -1.6 2,481,926 + PS417_11540 0.83 -2.3 2,481,926 + PS417_11540 0.83 +0.1 2,481,926 + PS417_11540 0.83 -0.9 2,481,927 - PS417_11540 0.83 +2.3 2,481,932 + PS417_11540 0.84 -1.2 2,481,933 - PS417_11540 0.84 +1.7 2,481,945 - PS417_11540 0.85 +2.1 2,482,061 + -0.2 2,482,061 + -0.0 2,482,402 + PS417_11545 0.29 -1.1 2,482,741 + PS417_11545 0.62 -1.8 2,482,742 - PS417_11545 0.62 -1.4 2,482,867 + PS417_11545 0.75 -3.0 2,483,117 + -0.5 2,483,511 + PS417_11550 0.33 -0.4 2,483,511 + PS417_11550 0.33 +0.2 2,483,599 + PS417_11550 0.41 +0.7 2,484,003 + PS417_11550 0.78 -1.5 2,484,071 + -1.3 2,484,071 + PS417_11550 0.84 -0.7 2,484,072 - PS417_11550 0.85 -0.7 2,484,111 + PS417_11550 0.88 -0.1
Or see this region's nucleotide sequence