Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_11345 and PS417_11350 are separated by 24 nucleotides PS417_11350 and PS417_11355 are separated by 11 nucleotides PS417_11355 and PS417_11360 are separated by 3 nucleotides
PS417_11345: PS417_11345 - membrane protein, at 2,443,306 to 2,444,889
_11345
PS417_11350: PS417_11350 - biopolymer transporter ExbB, at 2,444,914 to 2,446,713
_11350
PS417_11355: PS417_11355 - biopolymer transporter ExbD, at 2,446,725 to 2,447,150
_11355
PS417_11360: PS417_11360 - energy transducer TonB, at 2,447,154 to 2,447,858
_11360
Position (kb)
2444
2445
2446
2447 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2443.934 kb on - strand, within PS417_11345 at 2444.450 kb on - strand at 2444.656 kb on + strand, within PS417_11345 at 2444.656 kb on + strand, within PS417_11345 at 2444.657 kb on - strand, within PS417_11345 at 2444.657 kb on - strand, within PS417_11345 at 2445.271 kb on + strand, within PS417_11350 at 2445.272 kb on - strand, within PS417_11350 at 2445.272 kb on - strand, within PS417_11350 at 2445.272 kb on - strand, within PS417_11350 at 2445.331 kb on + strand, within PS417_11350 at 2446.169 kb on - strand, within PS417_11350 at 2447.038 kb on + strand at 2447.039 kb on - strand, within PS417_11355 at 2447.596 kb on - strand, within PS417_11360 at 2447.598 kb on - strand, within PS417_11360 at 2447.619 kb on + strand, within PS417_11360
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 2,443,934 - PS417_11345 0.40 -0.1 2,444,450 - -0.8 2,444,656 + PS417_11345 0.85 +1.4 2,444,656 + PS417_11345 0.85 +1.6 2,444,657 - PS417_11345 0.85 -0.3 2,444,657 - PS417_11345 0.85 -2.6 2,445,271 + PS417_11350 0.20 -0.5 2,445,272 - PS417_11350 0.20 -2.4 2,445,272 - PS417_11350 0.20 -0.2 2,445,272 - PS417_11350 0.20 -1.1 2,445,331 + PS417_11350 0.23 +0.0 2,446,169 - PS417_11350 0.70 -1.7 2,447,038 + +1.8 2,447,039 - PS417_11355 0.74 +1.3 2,447,596 - PS417_11360 0.63 +0.0 2,447,598 - PS417_11360 0.63 -2.9 2,447,619 + PS417_11360 0.66 +1.6
Or see this region's nucleotide sequence