Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_11135 and PS417_11140 overlap by 4 nucleotides PS417_11140 and PS417_11145 are separated by 2 nucleotides PS417_11145 and PS417_11150 are separated by 111 nucleotides
PS417_11135: PS417_11135 - peptide ABC transporter, at 2,386,960 to 2,387,901
_11135
PS417_11140: PS417_11140 - peptide ABC transporter permease, at 2,387,898 to 2,388,752
_11140
PS417_11145: PS417_11145 - peptide ABC transporter ATP-binding protein, at 2,388,755 to 2,390,383
_11145
PS417_11150: PS417_11150 - porin, at 2,390,495 to 2,391,778
_11150
Position (kb)
2388
2389
2390
2391 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2387.836 kb on + strand at 2387.836 kb on + strand at 2387.837 kb on - strand at 2387.837 kb on - strand at 2387.898 kb on - strand at 2388.363 kb on + strand, within PS417_11140 at 2388.424 kb on - strand at 2388.463 kb on - strand, within PS417_11140 at 2388.463 kb on - strand, within PS417_11140 at 2388.630 kb on + strand, within PS417_11140 at 2388.631 kb on - strand, within PS417_11140 at 2390.166 kb on - strand, within PS417_11145 at 2390.382 kb on - strand at 2390.454 kb on + strand at 2390.621 kb on + strand at 2390.622 kb on - strand at 2390.840 kb on + strand, within PS417_11150 at 2391.114 kb on - strand, within PS417_11150 at 2391.191 kb on + strand, within PS417_11150 at 2391.218 kb on + strand, within PS417_11150 at 2391.218 kb on + strand, within PS417_11150 at 2391.219 kb on - strand, within PS417_11150
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 2,387,836 + -1.3 2,387,836 + -0.6 2,387,837 - -3.5 2,387,837 - +0.9 2,387,898 - +1.8 2,388,363 + PS417_11140 0.54 +2.1 2,388,424 - +0.2 2,388,463 - PS417_11140 0.66 -0.8 2,388,463 - PS417_11140 0.66 +2.7 2,388,630 + PS417_11140 0.86 -0.6 2,388,631 - PS417_11140 0.86 +0.7 2,390,166 - PS417_11145 0.87 -2.2 2,390,382 - -1.4 2,390,454 + -0.4 2,390,621 + -0.1 2,390,622 - -1.6 2,390,840 + PS417_11150 0.27 +0.4 2,391,114 - PS417_11150 0.48 +0.3 2,391,191 + PS417_11150 0.54 +0.1 2,391,218 + PS417_11150 0.56 +0.4 2,391,218 + PS417_11150 0.56 -2.2 2,391,219 - PS417_11150 0.56 -1.5
Or see this region's nucleotide sequence