Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_11130 and PS417_11135 are separated by 13 nucleotides PS417_11135 and PS417_11140 overlap by 4 nucleotides PS417_11140 and PS417_11145 are separated by 2 nucleotides
PS417_11130: PS417_11130 - peptide ABC transporter substrate-binding protein, at 2,385,390 to 2,386,946
_11130
PS417_11135: PS417_11135 - peptide ABC transporter, at 2,386,960 to 2,387,901
_11135
PS417_11140: PS417_11140 - peptide ABC transporter permease, at 2,387,898 to 2,388,752
_11140
PS417_11145: PS417_11145 - peptide ABC transporter ATP-binding protein, at 2,388,755 to 2,390,383
_11145
Position (kb)
2386
2387
2388 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2385.987 kb on + strand, within PS417_11130 at 2385.987 kb on + strand, within PS417_11130 at 2385.988 kb on - strand, within PS417_11130 at 2385.992 kb on - strand, within PS417_11130 at 2386.014 kb on + strand, within PS417_11130 at 2386.621 kb on - strand, within PS417_11130 at 2386.642 kb on - strand, within PS417_11130 at 2387.459 kb on - strand, within PS417_11135 at 2387.836 kb on + strand at 2387.836 kb on + strand at 2387.837 kb on - strand at 2387.837 kb on - strand at 2387.898 kb on - strand at 2388.363 kb on + strand, within PS417_11140 at 2388.424 kb on - strand at 2388.463 kb on - strand, within PS417_11140 at 2388.463 kb on - strand, within PS417_11140 at 2388.630 kb on + strand, within PS417_11140 at 2388.631 kb on - strand, within PS417_11140
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 2,385,987 + PS417_11130 0.38 -0.3 2,385,987 + PS417_11130 0.38 +0.4 2,385,988 - PS417_11130 0.38 +1.0 2,385,992 - PS417_11130 0.39 +1.1 2,386,014 + PS417_11130 0.40 -0.6 2,386,621 - PS417_11130 0.79 +0.6 2,386,642 - PS417_11130 0.80 -1.2 2,387,459 - PS417_11135 0.53 -1.6 2,387,836 + -1.3 2,387,836 + -0.6 2,387,837 - -3.5 2,387,837 - +0.9 2,387,898 - +1.8 2,388,363 + PS417_11140 0.54 +2.1 2,388,424 - +0.2 2,388,463 - PS417_11140 0.66 -0.8 2,388,463 - PS417_11140 0.66 +2.7 2,388,630 + PS417_11140 0.86 -0.6 2,388,631 - PS417_11140 0.86 +0.7
Or see this region's nucleotide sequence