Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_10900 and PS417_10905 are separated by 47 nucleotides PS417_10905 and PS417_10910 overlap by 1 nucleotides PS417_10910 and PS417_10915 overlap by 4 nucleotides
PS417_10900: PS417_10900 - ABC transporter substrate-binding protein, at 2,331,362 to 2,332,138
_10900
PS417_10905: PS417_10905 - hypothetical protein, at 2,332,186 to 2,332,812
_10905
PS417_10910: PS417_10910 - FAD-dependent oxidoreductase, at 2,332,812 to 2,333,945
_10910
PS417_10915: PS417_10915 - ornithine cyclodeaminase, at 2,333,942 to 2,334,886
_10915
Position (kb)
2332
2333
2334 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2331.863 kb on + strand, within PS417_10900 at 2331.863 kb on + strand at 2331.864 kb on - strand, within PS417_10900 at 2332.127 kb on + strand at 2332.149 kb on - strand at 2332.149 kb on - strand at 2332.179 kb on - strand at 2332.179 kb on - strand at 2332.253 kb on + strand, within PS417_10905 at 2332.253 kb on + strand, within PS417_10905 at 2332.254 kb on - strand, within PS417_10905 at 2332.492 kb on - strand, within PS417_10905 at 2332.548 kb on + strand, within PS417_10905 at 2332.606 kb on + strand, within PS417_10905 at 2332.606 kb on + strand, within PS417_10905 at 2332.607 kb on - strand at 2332.657 kb on + strand, within PS417_10905 at 2332.657 kb on + strand, within PS417_10905 at 2332.658 kb on - strand, within PS417_10905 at 2332.688 kb on + strand, within PS417_10905 at 2332.688 kb on + strand, within PS417_10905 at 2332.688 kb on + strand, within PS417_10905 at 2332.689 kb on - strand, within PS417_10905 at 2332.845 kb on - strand at 2333.092 kb on + strand, within PS417_10910 at 2333.093 kb on - strand, within PS417_10910 at 2333.198 kb on - strand, within PS417_10910 at 2333.394 kb on + strand, within PS417_10910 at 2333.395 kb on - strand, within PS417_10910 at 2333.533 kb on + strand, within PS417_10910 at 2333.945 kb on + strand at 2333.945 kb on + strand at 2333.946 kb on - strand at 2333.946 kb on - strand at 2334.096 kb on + strand at 2334.096 kb on + strand, within PS417_10915 at 2334.097 kb on - strand, within PS417_10915 at 2334.286 kb on + strand, within PS417_10915 at 2334.450 kb on + strand, within PS417_10915 at 2334.451 kb on - strand, within PS417_10915 at 2334.451 kb on - strand at 2334.451 kb on - strand, within PS417_10915 at 2334.585 kb on - strand, within PS417_10915 at 2334.662 kb on - strand, within PS417_10915 at 2334.663 kb on + strand, within PS417_10915 at 2334.663 kb on + strand, within PS417_10915 at 2334.714 kb on + strand, within PS417_10915 at 2334.714 kb on + strand, within PS417_10915 at 2334.715 kb on - strand, within PS417_10915 at 2334.815 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 2,331,863 + PS417_10900 0.64 -0.6 2,331,863 + +0.2 2,331,864 - PS417_10900 0.65 +0.3 2,332,127 + +0.2 2,332,149 - -0.2 2,332,149 - -4.0 2,332,179 - +0.7 2,332,179 - -1.0 2,332,253 + PS417_10905 0.11 -0.0 2,332,253 + PS417_10905 0.11 +0.2 2,332,254 - PS417_10905 0.11 -2.1 2,332,492 - PS417_10905 0.49 +0.1 2,332,548 + PS417_10905 0.58 +0.6 2,332,606 + PS417_10905 0.67 -0.2 2,332,606 + PS417_10905 0.67 -2.0 2,332,607 - -0.8 2,332,657 + PS417_10905 0.75 +0.2 2,332,657 + PS417_10905 0.75 +2.8 2,332,658 - PS417_10905 0.75 -1.7 2,332,688 + PS417_10905 0.80 -3.6 2,332,688 + PS417_10905 0.80 +0.2 2,332,688 + PS417_10905 0.80 +1.3 2,332,689 - PS417_10905 0.80 -0.5 2,332,845 - +0.4 2,333,092 + PS417_10910 0.25 -1.4 2,333,093 - PS417_10910 0.25 -2.3 2,333,198 - PS417_10910 0.34 +0.6 2,333,394 + PS417_10910 0.51 -2.8 2,333,395 - PS417_10910 0.51 -3.2 2,333,533 + PS417_10910 0.64 -0.6 2,333,945 + +1.5 2,333,945 + -1.0 2,333,946 - +1.0 2,333,946 - -4.4 2,334,096 + -1.0 2,334,096 + PS417_10915 0.16 +1.2 2,334,097 - PS417_10915 0.16 -0.4 2,334,286 + PS417_10915 0.36 -0.3 2,334,450 + PS417_10915 0.54 +0.2 2,334,451 - PS417_10915 0.54 +0.8 2,334,451 - +0.8 2,334,451 - PS417_10915 0.54 -0.2 2,334,585 - PS417_10915 0.68 +0.7 2,334,662 - PS417_10915 0.76 -0.8 2,334,663 + PS417_10915 0.76 -0.4 2,334,663 + PS417_10915 0.76 -0.8 2,334,714 + PS417_10915 0.82 -1.0 2,334,714 + PS417_10915 0.82 -1.9 2,334,715 - PS417_10915 0.82 +0.5 2,334,815 - -3.6
Or see this region's nucleotide sequence