Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_01060 and PS417_01065 overlap by 11 nucleotides PS417_01065 and PS417_01070 overlap by 4 nucleotides PS417_01070 and PS417_01075 are separated by 53 nucleotides
PS417_01060: PS417_01060 - ABC transporter permease, at 232,560 to 233,204
_01060
PS417_01065: PS417_01065 - ABC transporter, at 233,194 to 234,309
_01065
PS417_01070: PS417_01070 - methionine ABC transporter substrate-binding protein, at 234,306 to 235,121
_01070
PS417_01075: PS417_01075 - N5,N10-methylene tetrahydromethanopterin reductase, at 235,175 to 236,527
_01075
Position (kb) 233
234
235 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 232.244 kb on - strand at 232.284 kb on + strand at 232.284 kb on + strand at 232.285 kb on - strand at 232.285 kb on - strand at 232.285 kb on - strand at 232.302 kb on + strand at 232.313 kb on + strand at 232.313 kb on + strand at 232.314 kb on - strand at 232.314 kb on - strand at 232.315 kb on + strand at 232.315 kb on + strand at 232.316 kb on - strand at 232.316 kb on - strand at 232.321 kb on + strand at 232.321 kb on + strand at 232.349 kb on + strand at 232.349 kb on + strand at 232.350 kb on - strand at 232.350 kb on - strand at 232.557 kb on + strand at 232.558 kb on - strand at 232.558 kb on - strand at 232.955 kb on - strand, within PS417_01060 at 233.075 kb on - strand, within PS417_01060 at 233.075 kb on - strand, within PS417_01060 at 233.075 kb on - strand, within PS417_01060 at 233.075 kb on - strand, within PS417_01060 at 233.078 kb on + strand, within PS417_01060 at 233.078 kb on + strand, within PS417_01060 at 233.078 kb on + strand, within PS417_01060 at 233.079 kb on - strand, within PS417_01060 at 233.079 kb on - strand, within PS417_01060 at 233.320 kb on - strand, within PS417_01065 at 234.388 kb on + strand, within PS417_01070 at 234.389 kb on - strand, within PS417_01070 at 234.458 kb on - strand, within PS417_01070 at 234.458 kb on - strand, within PS417_01070 at 234.458 kb on - strand, within PS417_01070 at 234.458 kb on - strand, within PS417_01070 at 234.526 kb on + strand, within PS417_01070 at 234.527 kb on - strand, within PS417_01070 at 234.527 kb on - strand, within PS417_01070 at 234.527 kb on - strand, within PS417_01070 at 234.527 kb on - strand, within PS417_01070 at 234.646 kb on + strand, within PS417_01070 at 234.647 kb on - strand, within PS417_01070 at 234.651 kb on + strand, within PS417_01070 at 234.772 kb on + strand, within PS417_01070 at 234.772 kb on + strand, within PS417_01070 at 234.773 kb on - strand, within PS417_01070 at 234.880 kb on + strand, within PS417_01070 at 234.880 kb on + strand, within PS417_01070 at 234.957 kb on - strand, within PS417_01070 at 235.139 kb on - strand at 235.149 kb on + strand at 235.150 kb on - strand at 235.150 kb on - strand at 235.150 kb on - strand at 235.185 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 232,244 - +0.6 232,284 + -1.0 232,284 + +0.5 232,285 - -0.1 232,285 - -0.4 232,285 - -0.4 232,302 + +1.1 232,313 + -0.4 232,313 + +0.7 232,314 - -1.4 232,314 - +0.1 232,315 + +0.5 232,315 + -3.7 232,316 - +0.0 232,316 - -0.6 232,321 + +0.2 232,321 + -1.8 232,349 + +1.0 232,349 + -3.4 232,350 - -3.1 232,350 - -0.2 232,557 + -0.5 232,558 - +1.8 232,558 - -1.9 232,955 - PS417_01060 0.61 +0.1 233,075 - PS417_01060 0.80 -2.4 233,075 - PS417_01060 0.80 +0.2 233,075 - PS417_01060 0.80 +1.2 233,075 - PS417_01060 0.80 +2.0 233,078 + PS417_01060 0.80 -1.3 233,078 + PS417_01060 0.80 -1.5 233,078 + PS417_01060 0.80 +0.3 233,079 - PS417_01060 0.80 -2.3 233,079 - PS417_01060 0.80 -1.7 233,320 - PS417_01065 0.11 -1.2 234,388 + PS417_01070 0.10 -0.1 234,389 - PS417_01070 0.10 -2.3 234,458 - PS417_01070 0.19 -1.2 234,458 - PS417_01070 0.19 +0.7 234,458 - PS417_01070 0.19 +0.4 234,458 - PS417_01070 0.19 +0.0 234,526 + PS417_01070 0.27 -1.0 234,527 - PS417_01070 0.27 -2.7 234,527 - PS417_01070 0.27 -1.0 234,527 - PS417_01070 0.27 +0.3 234,527 - PS417_01070 0.27 -3.2 234,646 + PS417_01070 0.42 -0.5 234,647 - PS417_01070 0.42 -1.0 234,651 + PS417_01070 0.42 -0.9 234,772 + PS417_01070 0.57 +1.1 234,772 + PS417_01070 0.57 -2.3 234,773 - PS417_01070 0.57 -0.1 234,880 + PS417_01070 0.70 -0.4 234,880 + PS417_01070 0.70 +0.2 234,957 - PS417_01070 0.80 -1.4 235,139 - +1.4 235,149 + -0.5 235,150 - +1.8 235,150 - +2.0 235,150 - -1.8 235,185 + +0.2
Or see this region's nucleotide sequence