Strain Fitness in Pseudomonas simiae WCS417 around PS417_00110

Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPS417_00110 and PS417_00115 are separated by 130 nucleotides PS417_00110: PS417_00110 - tRNA threonylcarbamoyladenosine biosynthesis protein RimN, at 23,683 to 24,240 _00110 PS417_00115: PS417_00115 - quinone oxidoreductase, at 24,371 to 25,348 _00115 Position (kb) 23 24 25Strain fitness (log2 ratio) -2 -1 0 1 2 3at 22.863 kb on - strandat 23.317 kb on + strandat 23.318 kb on - strandat 23.318 kb on - strandat 23.401 kb on - strandat 23.627 kb on - strandat 23.665 kb on + strandat 24.364 kb on + strandat 24.364 kb on + strandat 24.364 kb on + strandat 24.364 kb on + strandat 24.365 kb on - strandat 24.365 kb on - strandat 24.365 kb on - strandat 24.365 kb on - strandat 24.365 kb on - strandat 24.365 kb on - strandat 24.366 kb on + strandat 24.397 kb on + strandat 24.526 kb on + strand, within PS417_00115at 24.526 kb on + strand, within PS417_00115at 24.527 kb on - strand, within PS417_00115at 24.527 kb on - strand, within PS417_00115at 24.543 kb on + strand, within PS417_00115at 24.570 kb on + strand, within PS417_00115at 24.571 kb on - strand, within PS417_00115at 24.709 kb on - strand, within PS417_00115at 24.942 kb on + strand, within PS417_00115at 24.942 kb on + strand, within PS417_00115at 24.942 kb on + strand, within PS417_00115at 24.943 kb on - strand, within PS417_00115at 24.943 kb on - strand, within PS417_00115at 25.065 kb on + strand, within PS417_00115at 25.212 kb on + strand, within PS417_00115at 25.213 kb on - strand, within PS417_00115

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml
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22,863 - -0.6
23,317 + +1.5
23,318 - -1.2
23,318 - -2.4
23,401 - -0.4
23,627 - -0.1
23,665 + +0.4
24,364 + +2.2
24,364 + +0.1
24,364 + -2.1
24,364 + +2.0
24,365 - -1.0
24,365 - -0.8
24,365 - -2.6
24,365 - -0.7
24,365 - -0.2
24,365 - +1.8
24,366 + -1.1
24,397 + -0.3
24,526 + PS417_00115 0.16 +2.6
24,526 + PS417_00115 0.16 -2.0
24,527 - PS417_00115 0.16 -2.0
24,527 - PS417_00115 0.16 +2.4
24,543 + PS417_00115 0.18 +0.8
24,570 + PS417_00115 0.20 +0.1
24,571 - PS417_00115 0.20 +0.5
24,709 - PS417_00115 0.35 -1.1
24,942 + PS417_00115 0.58 +1.7
24,942 + PS417_00115 0.58 -0.4
24,942 + PS417_00115 0.58 +3.0
24,943 - PS417_00115 0.58 +1.8
24,943 - PS417_00115 0.58 +1.2
25,065 + PS417_00115 0.71 +1.2
25,212 + PS417_00115 0.86 +2.1
25,213 - PS417_00115 0.86 +0.4

Or see this region's nucleotide sequence