Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_10360 and PS417_10365 are separated by 105 nucleotides PS417_10365 and PS417_10370 overlap by 30 nucleotides PS417_10370 and PS417_10375 overlap by 4 nucleotides PS417_10375 and PS417_10380 are separated by 5 nucleotides
PS417_10360: PS417_10360 - MFS transporter, at 2,216,511 to 2,217,725
_10360
PS417_10365: PS417_10365 - AraC family transcriptional regulator, at 2,217,831 to 2,218,646
_10365
PS417_10370: PS417_10370 - ATPase, at 2,218,617 to 2,219,963
_10370
PS417_10375: PS417_10375 - chemotaxis protein CheY, at 2,219,960 to 2,220,700
_10375
PS417_10380: PS417_10380 - catalase, at 2,220,706 to 2,222,139
_10380
Position (kb)
2218
2219
2220 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2217.766 kb on - strand at 2217.798 kb on - strand at 2217.919 kb on + strand, within PS417_10365 at 2217.920 kb on - strand, within PS417_10365 at 2217.996 kb on + strand, within PS417_10365 at 2217.997 kb on - strand, within PS417_10365 at 2217.997 kb on - strand, within PS417_10365 at 2218.131 kb on + strand, within PS417_10365 at 2218.132 kb on - strand at 2218.239 kb on + strand at 2218.401 kb on + strand, within PS417_10365 at 2218.401 kb on + strand, within PS417_10365 at 2218.402 kb on - strand, within PS417_10365 at 2218.402 kb on - strand, within PS417_10365 at 2218.566 kb on + strand at 2218.711 kb on + strand at 2218.814 kb on - strand, within PS417_10370 at 2219.319 kb on - strand, within PS417_10370 at 2219.339 kb on - strand, within PS417_10370 at 2219.726 kb on - strand, within PS417_10370 at 2219.861 kb on - strand at 2220.003 kb on + strand at 2220.003 kb on + strand at 2220.003 kb on + strand at 2220.180 kb on - strand, within PS417_10375 at 2220.679 kb on + strand at 2220.679 kb on + strand at 2220.680 kb on - strand at 2220.713 kb on - strand at 2220.746 kb on + strand at 2220.778 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 2,217,766 - +0.3 2,217,798 - +1.0 2,217,919 + PS417_10365 0.11 +0.1 2,217,920 - PS417_10365 0.11 -0.2 2,217,996 + PS417_10365 0.20 +0.1 2,217,997 - PS417_10365 0.20 +2.4 2,217,997 - PS417_10365 0.20 +0.8 2,218,131 + PS417_10365 0.37 +1.6 2,218,132 - -1.0 2,218,239 + +2.5 2,218,401 + PS417_10365 0.70 -0.5 2,218,401 + PS417_10365 0.70 +0.6 2,218,402 - PS417_10365 0.70 +0.2 2,218,402 - PS417_10365 0.70 -1.8 2,218,566 + -0.6 2,218,711 + +0.3 2,218,814 - PS417_10370 0.15 -0.4 2,219,319 - PS417_10370 0.52 -0.1 2,219,339 - PS417_10370 0.54 -2.3 2,219,726 - PS417_10370 0.82 -1.2 2,219,861 - +0.4 2,220,003 + -1.9 2,220,003 + +1.3 2,220,003 + -0.3 2,220,180 - PS417_10375 0.30 +1.1 2,220,679 + +1.2 2,220,679 + -1.1 2,220,680 - -0.4 2,220,713 - -3.8 2,220,746 + +1.2 2,220,778 - +0.4
Or see this region's nucleotide sequence