Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_10355 and PS417_10360 are separated by 90 nucleotides PS417_10360 and PS417_10365 are separated by 105 nucleotides PS417_10365 and PS417_10370 overlap by 30 nucleotides
PS417_10355: PS417_10355 - transcriptional regulator, at 2,213,550 to 2,216,420
_10355
PS417_10360: PS417_10360 - MFS transporter, at 2,216,511 to 2,217,725
_10360
PS417_10365: PS417_10365 - AraC family transcriptional regulator, at 2,217,831 to 2,218,646
_10365
PS417_10370: PS417_10370 - ATPase, at 2,218,617 to 2,219,963
_10370
Position (kb)
2216
2217
2218 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2216.126 kb on + strand, within PS417_10355 at 2216.126 kb on + strand, within PS417_10355 at 2216.451 kb on + strand at 2216.491 kb on + strand at 2216.633 kb on - strand, within PS417_10360 at 2216.633 kb on - strand, within PS417_10360 at 2216.633 kb on - strand, within PS417_10360 at 2216.815 kb on + strand, within PS417_10360 at 2216.816 kb on - strand at 2216.816 kb on - strand, within PS417_10360 at 2217.074 kb on - strand, within PS417_10360 at 2217.220 kb on - strand, within PS417_10360 at 2217.301 kb on - strand, within PS417_10360 at 2217.766 kb on - strand at 2217.798 kb on - strand at 2217.919 kb on + strand, within PS417_10365 at 2217.920 kb on - strand, within PS417_10365 at 2217.996 kb on + strand, within PS417_10365 at 2217.997 kb on - strand, within PS417_10365 at 2217.997 kb on - strand, within PS417_10365 at 2218.131 kb on + strand, within PS417_10365 at 2218.132 kb on - strand at 2218.239 kb on + strand at 2218.401 kb on + strand, within PS417_10365 at 2218.401 kb on + strand, within PS417_10365 at 2218.402 kb on - strand, within PS417_10365 at 2218.402 kb on - strand, within PS417_10365 at 2218.566 kb on + strand at 2218.711 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 2,216,126 + PS417_10355 0.90 +0.9 2,216,126 + PS417_10355 0.90 -0.6 2,216,451 + -2.4 2,216,491 + +1.8 2,216,633 - PS417_10360 0.10 -1.1 2,216,633 - PS417_10360 0.10 +0.7 2,216,633 - PS417_10360 0.10 -1.4 2,216,815 + PS417_10360 0.25 +1.4 2,216,816 - -0.2 2,216,816 - PS417_10360 0.25 +2.2 2,217,074 - PS417_10360 0.46 +0.8 2,217,220 - PS417_10360 0.58 -0.6 2,217,301 - PS417_10360 0.65 +1.6 2,217,766 - +0.3 2,217,798 - +1.0 2,217,919 + PS417_10365 0.11 +0.1 2,217,920 - PS417_10365 0.11 -0.2 2,217,996 + PS417_10365 0.20 +0.1 2,217,997 - PS417_10365 0.20 +2.4 2,217,997 - PS417_10365 0.20 +0.8 2,218,131 + PS417_10365 0.37 +1.6 2,218,132 - -1.0 2,218,239 + +2.5 2,218,401 + PS417_10365 0.70 -0.5 2,218,401 + PS417_10365 0.70 +0.6 2,218,402 - PS417_10365 0.70 +0.2 2,218,402 - PS417_10365 0.70 -1.8 2,218,566 + -0.6 2,218,711 + +0.3
Or see this region's nucleotide sequence