Strain Fitness in Pseudomonas simiae WCS417 around PS417_10355

Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	R2A with Polymyxin B sulfate 0.003 mg/ml
2,212,850	+		PS417_10350	0.46	-1.5
2,212,859	+		PS417_10350	0.47	-0.5
2,212,859	+		PS417_10350	0.47	-0.6
2,212,859	+		PS417_10350	0.47	+0.8
2,212,859	+		PS417_10350	0.47	-0.3
2,212,860	-		PS417_10350	0.47	-0.5
2,212,860	-		PS417_10350	0.47	+0.2
2,213,040	-		PS417_10350	0.61	-2.9
2,213,247	-		PS417_10350	0.77	-0.4
2,213,306	-		PS417_10350	0.82	-0.2
2,213,306	-		PS417_10350	0.82	-0.5
2,213,306	-		PS417_10350	0.82	-2.1
2,213,306	-		PS417_10350	0.82	-0.5
2,213,429	+				-0.1
2,213,429	+				+0.2
2,213,430	-				-1.0
2,213,432	+				-0.2
2,213,433	-				+0.2
2,213,433	-				+0.5
2,213,433	-				+0.0
2,213,434	+				-2.4
2,213,647	+				+0.5
2,213,647	+				+0.3
2,214,082	+		PS417_10355	0.19	+0.0
2,214,083	-		PS417_10355	0.19	-0.2
2,214,167	-		PS417_10355	0.21	-1.5
2,214,200	-		PS417_10355	0.23	-1.3
2,214,344	+		PS417_10355	0.28	-0.6
2,214,427	+		PS417_10355	0.31	+0.1
2,214,527	+		PS417_10355	0.34	+1.1
2,214,528	-		PS417_10355	0.34	+1.0
2,214,547	+		PS417_10355	0.35	+0.2
2,214,694	-		PS417_10355	0.40	-0.9
2,214,722	-		PS417_10355	0.41	-0.3
2,215,203	+		PS417_10355	0.58	+0.0
2,215,253	-				-0.2
2,215,359	+		PS417_10355	0.63	-0.0
2,215,359	+				-0.8
2,215,359	+		PS417_10355	0.63	+0.3
2,215,359	+		PS417_10355	0.63	-0.4
2,215,360	-				-0.1
2,216,126	+		PS417_10355	0.90	+0.9
2,216,126	+		PS417_10355	0.90	-0.6
2,216,451	+				-2.4
2,216,491	+				+1.8
2,216,633	-		PS417_10360	0.10	-1.1
2,216,633	-		PS417_10360	0.10	+0.7
2,216,633	-		PS417_10360	0.10	-1.4
2,216,815	+		PS417_10360	0.25	+1.4
2,216,816	-				-0.2
2,216,816	-		PS417_10360	0.25	+2.2
2,217,074	-		PS417_10360	0.46	+0.8
2,217,220	-		PS417_10360	0.58	-0.6
2,217,301	-		PS417_10360	0.65	+1.6