Strain Fitness in Pseudomonas simiae WCS417 around PS417_10350

Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	R2A with Polymyxin B sulfate 0.003 mg/ml
2,211,355	+		PS417_10345	0.41	+1.3
2,211,647	+		PS417_10345	0.65	-0.6
2,211,682	-		PS417_10345	0.68	-3.1
2,211,682	-		PS417_10345	0.68	-0.8
2,211,716	+		PS417_10345	0.71	+0.0
2,211,716	+		PS417_10345	0.71	-0.8
2,212,009	-				-1.5
2,212,142	-				+2.0
2,212,163	+				-0.2
2,212,163	+				+1.4
2,212,163	+				-0.1
2,212,163	+				+0.3
2,212,164	-				+0.7
2,212,183	-				-1.4
2,212,370	-				+0.7
2,212,850	+		PS417_10350	0.46	-1.5
2,212,859	+		PS417_10350	0.47	-0.5
2,212,859	+		PS417_10350	0.47	-0.6
2,212,859	+		PS417_10350	0.47	+0.8
2,212,859	+		PS417_10350	0.47	-0.3
2,212,860	-		PS417_10350	0.47	-0.5
2,212,860	-		PS417_10350	0.47	+0.2
2,213,040	-		PS417_10350	0.61	-2.9
2,213,247	-		PS417_10350	0.77	-0.4
2,213,306	-		PS417_10350	0.82	-0.2
2,213,306	-		PS417_10350	0.82	-0.5
2,213,306	-		PS417_10350	0.82	-2.1
2,213,306	-		PS417_10350	0.82	-0.5
2,213,429	+				-0.1
2,213,429	+				+0.2
2,213,430	-				-1.0
2,213,432	+				-0.2
2,213,433	-				+0.2
2,213,433	-				+0.5
2,213,433	-				+0.0
2,213,434	+				-2.4
2,213,647	+				+0.5
2,213,647	+				+0.3
2,214,082	+		PS417_10355	0.19	+0.0
2,214,083	-		PS417_10355	0.19	-0.2
2,214,167	-		PS417_10355	0.21	-1.5
2,214,200	-		PS417_10355	0.23	-1.3
2,214,344	+		PS417_10355	0.28	-0.6
2,214,427	+		PS417_10355	0.31	+0.1
2,214,527	+		PS417_10355	0.34	+1.1
2,214,528	-		PS417_10355	0.34	+1.0