Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_10255 and PS417_10260 are separated by 157 nucleotides PS417_10260 and PS417_10265 overlap by 1 nucleotides PS417_10265 and PS417_10270 overlap by 1 nucleotides PS417_10270 and PS417_10275 overlap by 4 nucleotides
PS417_10255: PS417_10255 - hypothetical protein, at 2,192,710 to 2,193,789
_10255
PS417_10260: PS417_10260 - peptidase, at 2,193,947 to 2,194,249
_10260
PS417_10265: PS417_10265 - peptidase, at 2,194,249 to 2,194,560
_10265
PS417_10270: PS417_10270 - transcriptional regulator, at 2,194,560 to 2,195,228
_10270
PS417_10275: PS417_10275 - histidine kinase, at 2,195,225 to 2,196,544
_10275
Position (kb)
2193
2194
2195 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2193.115 kb on + strand, within PS417_10255 at 2193.116 kb on - strand, within PS417_10255 at 2193.372 kb on + strand, within PS417_10255 at 2193.372 kb on + strand, within PS417_10255 at 2193.372 kb on + strand, within PS417_10255 at 2193.373 kb on - strand, within PS417_10255 at 2193.373 kb on - strand, within PS417_10255 at 2193.498 kb on - strand, within PS417_10255 at 2193.648 kb on + strand, within PS417_10255 at 2193.723 kb on + strand at 2193.790 kb on - strand at 2193.820 kb on - strand at 2193.821 kb on + strand at 2193.890 kb on - strand at 2193.943 kb on + strand at 2193.943 kb on + strand at 2193.943 kb on + strand at 2193.943 kb on + strand at 2193.944 kb on - strand at 2193.944 kb on - strand at 2193.944 kb on - strand at 2193.944 kb on - strand at 2193.944 kb on - strand at 2193.979 kb on + strand, within PS417_10260 at 2194.051 kb on + strand, within PS417_10260 at 2194.052 kb on - strand, within PS417_10260 at 2194.054 kb on + strand, within PS417_10260 at 2194.055 kb on - strand, within PS417_10260 at 2194.055 kb on - strand, within PS417_10260 at 2194.055 kb on - strand, within PS417_10260 at 2194.139 kb on + strand, within PS417_10260 at 2194.148 kb on + strand, within PS417_10260 at 2194.148 kb on + strand, within PS417_10260 at 2194.149 kb on - strand, within PS417_10260 at 2194.154 kb on + strand, within PS417_10260 at 2194.154 kb on + strand, within PS417_10260 at 2194.248 kb on - strand at 2194.263 kb on - strand at 2194.330 kb on - strand, within PS417_10265 at 2194.463 kb on - strand, within PS417_10265 at 2194.463 kb on - strand, within PS417_10265 at 2194.469 kb on - strand, within PS417_10265 at 2194.692 kb on + strand, within PS417_10270 at 2194.852 kb on - strand at 2194.884 kb on + strand, within PS417_10270 at 2195.040 kb on + strand, within PS417_10270 at 2195.097 kb on + strand, within PS417_10270 at 2195.097 kb on + strand, within PS417_10270
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 2,193,115 + PS417_10255 0.38 +1.1 2,193,116 - PS417_10255 0.38 -1.4 2,193,372 + PS417_10255 0.61 +1.0 2,193,372 + PS417_10255 0.61 +1.5 2,193,372 + PS417_10255 0.61 +0.2 2,193,373 - PS417_10255 0.61 +0.2 2,193,373 - PS417_10255 0.61 +0.4 2,193,498 - PS417_10255 0.73 +2.1 2,193,648 + PS417_10255 0.87 -1.5 2,193,723 + -0.0 2,193,790 - +0.8 2,193,820 - -0.2 2,193,821 + +1.4 2,193,890 - -0.5 2,193,943 + -2.9 2,193,943 + +0.2 2,193,943 + +1.3 2,193,943 + -0.2 2,193,944 - +0.0 2,193,944 - -1.7 2,193,944 - +2.0 2,193,944 - +0.8 2,193,944 - -0.2 2,193,979 + PS417_10260 0.11 -1.5 2,194,051 + PS417_10260 0.34 -0.3 2,194,052 - PS417_10260 0.35 -1.0 2,194,054 + PS417_10260 0.35 -0.5 2,194,055 - PS417_10260 0.36 -0.2 2,194,055 - PS417_10260 0.36 +0.9 2,194,055 - PS417_10260 0.36 +2.7 2,194,139 + PS417_10260 0.63 -0.7 2,194,148 + PS417_10260 0.66 -0.6 2,194,148 + PS417_10260 0.66 -0.5 2,194,149 - PS417_10260 0.67 +1.0 2,194,154 + PS417_10260 0.68 +0.3 2,194,154 + PS417_10260 0.68 +0.6 2,194,248 - +1.3 2,194,263 - +0.2 2,194,330 - PS417_10265 0.26 +2.2 2,194,463 - PS417_10265 0.69 -0.2 2,194,463 - PS417_10265 0.69 +0.5 2,194,469 - PS417_10265 0.71 +0.9 2,194,692 + PS417_10270 0.20 -0.5 2,194,852 - -1.7 2,194,884 + PS417_10270 0.48 +1.1 2,195,040 + PS417_10270 0.72 +0.0 2,195,097 + PS417_10270 0.80 +1.0 2,195,097 + PS417_10270 0.80 +0.4
Or see this region's nucleotide sequence