Strain Fitness in Pseudomonas simiae WCS417 around PS417_10250

Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	R2A with Polymyxin B sulfate 0.003 mg/ml
2,189,385	+		PS417_10240	0.45	+0.9
2,189,386	-		PS417_10240	0.45	+0.1
2,189,387	+		PS417_10240	0.45	-1.4
2,189,659	+				-0.5
2,189,660	-		PS417_10240	0.85	-0.8
2,189,660	-		PS417_10240	0.85	+0.6
2,189,660	-		PS417_10240	0.85	-0.8
2,189,789	+				-2.7
2,189,789	+				+0.2
2,189,789	+				-2.7
2,189,790	-				-1.2
2,189,790	-				-0.1
2,189,796	-				+0.2
2,189,796	-				-1.8
2,189,833	+		PS417_10245	0.11	+2.1
2,190,099	-		PS417_10245	0.84	-1.0
2,190,115	-		PS417_10245	0.88	-2.7
2,190,163	+				-2.0
2,190,181	-				+1.1
2,190,376	+				-0.0
2,190,441	+				-2.1
2,190,442	-				-2.2
2,190,445	+				-0.8
2,190,446	-				+2.0
2,190,458	+				-0.2
2,190,459	-				-1.5
2,190,461	-				+0.9
2,190,461	-				-1.1
2,190,461	-				+0.3
2,190,461	-				-1.7
2,190,461	-				-0.2
2,190,461	-				-2.5
2,190,462	+				-1.0
2,190,462	+				-3.0
2,190,462	+				+0.3
2,190,462	+				-1.2
2,190,462	+				+0.5
2,190,462	+				+0.4
2,190,463	-				+0.8
2,190,480	-				+1.4
2,190,480	-				-0.5
2,190,620	-		PS417_10250	0.21	+0.6
2,190,847	+		PS417_10250	0.40	+0.7
2,190,848	-		PS417_10250	0.40	+2.5
2,190,848	-		PS417_10250	0.40	+1.5
2,191,030	+		PS417_10250	0.55	+1.6
2,191,030	+		PS417_10250	0.55	-0.5
2,191,030	+		PS417_10250	0.55	+2.8
2,191,031	-		PS417_10250	0.55	-1.0
2,191,060	-		PS417_10250	0.58	-0.7
2,191,201	+		PS417_10250	0.70	-0.3
2,191,201	+		PS417_10250	0.70	-0.9
2,191,358	+		PS417_10250	0.83	-0.7
2,191,434	-		PS417_10250	0.89	+0.1
2,191,455	+				-0.5
2,191,474	+				-1.0
2,191,474	+				+0.2
2,191,474	+				+1.5
2,191,474	+				-2.7
2,191,474	+				-2.9
2,191,475	-				+0.3
2,191,475	-				-0.2
2,191,507	+				-0.0
2,191,563	+				+1.7
2,191,563	+				-0.5
2,191,563	+				+0.0
2,191,564	-				-0.3
2,191,594	-				+1.0
2,191,605	+				-0.0
2,191,605	+				+1.2
2,191,605	+				-0.1
2,191,605	+				+0.1
2,191,605	+				-1.2
2,191,606	-				+0.3
2,191,606	-				-2.3
2,191,606	-				-2.8
2,191,608	+				-0.5
2,191,609	-				+0.4
2,191,630	+				-0.2
2,191,630	+				-0.3
2,191,641	-				-1.7
2,191,656	+				-1.2
2,191,824	-				-0.5
2,191,847	+				-0.6
2,191,847	+				-0.5
2,191,874	+				-2.1
2,191,906	+				-2.4
2,191,914	-				-0.2
2,192,057	-				+1.5
2,192,057	-				-0.5
2,192,125	-				-0.5
2,192,125	-				+2.3
2,192,198	-				+1.1
2,192,198	-				-1.8
2,192,198	-				-1.5
2,192,202	-				-3.3
2,192,270	+				-0.3
2,192,271	-				+1.3
2,192,271	-				-1.9
2,192,274	+				-2.4
2,192,342	+				-0.2
2,192,342	+				+1.7
2,192,431	+				-1.2
2,192,431	+				+1.3
2,192,431	+				+1.3
2,192,431	+				-1.8
2,192,431	+				+1.1
2,192,431	+				-0.6
2,192,433	+				-0.1
2,192,433	+				-1.8
2,192,444	+				+1.0
2,192,444	+				-3.2