Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_10035 and PS417_10040 are separated by 200 nucleotides PS417_10040 and PS417_10045 overlap by 4 nucleotides PS417_10045 and PS417_10050 are separated by 119 nucleotides PS417_10050 and PS417_10055 are separated by 61 nucleotides PS417_10055 and PS417_10060 overlap by 4 nucleotides
PS417_10035: PS417_10035 - hypothetical protein, at 2,156,842 to 2,157,603
_10035
PS417_10040: PS417_10040 - MFS transporter, at 2,157,804 to 2,158,142
_10040
PS417_10045: PS417_10045 - hypothetical protein, at 2,158,139 to 2,158,483
_10045
PS417_10050: PS417_10050 - hypothetical protein, at 2,158,603 to 2,158,794
_10050
PS417_10055: PS417_10055 - hypothetical protein, at 2,158,856 to 2,159,047
_10055
PS417_10060: PS417_10060 - hypothetical protein, at 2,159,044 to 2,159,298
_10060
Position (kb)
2157
2158
2159 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2156.893 kb on + strand at 2156.893 kb on + strand at 2156.912 kb on + strand at 2156.913 kb on - strand at 2156.916 kb on + strand at 2156.981 kb on - strand, within PS417_10035 at 2156.990 kb on + strand, within PS417_10035 at 2156.991 kb on - strand, within PS417_10035 at 2156.991 kb on - strand, within PS417_10035 at 2157.069 kb on + strand at 2157.076 kb on - strand, within PS417_10035 at 2157.096 kb on - strand, within PS417_10035 at 2157.096 kb on - strand, within PS417_10035 at 2157.161 kb on - strand at 2157.192 kb on + strand, within PS417_10035 at 2157.193 kb on - strand, within PS417_10035 at 2157.251 kb on - strand, within PS417_10035 at 2157.251 kb on - strand, within PS417_10035 at 2157.277 kb on + strand, within PS417_10035 at 2157.277 kb on + strand, within PS417_10035 at 2157.277 kb on + strand, within PS417_10035 at 2157.278 kb on - strand, within PS417_10035 at 2157.370 kb on + strand, within PS417_10035 at 2157.577 kb on + strand at 2157.603 kb on + strand at 2157.603 kb on + strand at 2157.603 kb on + strand at 2157.604 kb on - strand at 2157.604 kb on - strand at 2157.617 kb on + strand at 2157.617 kb on + strand at 2157.617 kb on + strand at 2157.618 kb on - strand at 2157.656 kb on - strand at 2157.773 kb on + strand at 2157.810 kb on + strand at 2157.810 kb on + strand at 2157.811 kb on - strand at 2157.814 kb on + strand at 2157.814 kb on + strand at 2157.963 kb on + strand, within PS417_10040 at 2158.203 kb on + strand, within PS417_10045 at 2158.247 kb on + strand, within PS417_10045 at 2158.280 kb on - strand, within PS417_10045 at 2158.555 kb on + strand at 2158.555 kb on + strand at 2158.555 kb on + strand at 2158.575 kb on + strand at 2158.603 kb on - strand at 2158.603 kb on - strand at 2158.603 kb on - strand at 2158.633 kb on - strand, within PS417_10050 at 2158.653 kb on - strand, within PS417_10050 at 2158.779 kb on + strand at 2158.780 kb on - strand at 2158.780 kb on - strand at 2158.855 kb on + strand at 2158.856 kb on - strand at 2158.904 kb on - strand, within PS417_10055
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 2,156,893 + +1.5 2,156,893 + +0.6 2,156,912 + -0.8 2,156,913 - -0.6 2,156,916 + +0.2 2,156,981 - PS417_10035 0.18 +0.4 2,156,990 + PS417_10035 0.19 -0.2 2,156,991 - PS417_10035 0.20 -0.1 2,156,991 - PS417_10035 0.20 -0.4 2,157,069 + +1.0 2,157,076 - PS417_10035 0.31 +0.8 2,157,096 - PS417_10035 0.33 +0.9 2,157,096 - PS417_10035 0.33 -3.4 2,157,161 - +0.2 2,157,192 + PS417_10035 0.46 -1.6 2,157,193 - PS417_10035 0.46 -1.4 2,157,251 - PS417_10035 0.54 -2.1 2,157,251 - PS417_10035 0.54 +0.2 2,157,277 + PS417_10035 0.57 +0.8 2,157,277 + PS417_10035 0.57 -0.8 2,157,277 + PS417_10035 0.57 -3.3 2,157,278 - PS417_10035 0.57 +0.6 2,157,370 + PS417_10035 0.69 -0.7 2,157,577 + -3.7 2,157,603 + +0.3 2,157,603 + -1.2 2,157,603 + -0.1 2,157,604 - -2.0 2,157,604 - +0.3 2,157,617 + -1.0 2,157,617 + -0.8 2,157,617 + +0.1 2,157,618 - -1.8 2,157,656 - -1.7 2,157,773 + -2.5 2,157,810 + -1.0 2,157,810 + -0.2 2,157,811 - -0.1 2,157,814 + -0.0 2,157,814 + +0.9 2,157,963 + PS417_10040 0.47 -2.5 2,158,203 + PS417_10045 0.19 +0.4 2,158,247 + PS417_10045 0.31 +0.6 2,158,280 - PS417_10045 0.41 +0.2 2,158,555 + -1.7 2,158,555 + +0.5 2,158,555 + -1.4 2,158,575 + -0.1 2,158,603 - -2.1 2,158,603 - -0.2 2,158,603 - -0.3 2,158,633 - PS417_10050 0.16 +0.5 2,158,653 - PS417_10050 0.26 -1.0 2,158,779 + -1.2 2,158,780 - -0.2 2,158,780 - -0.8 2,158,855 + +0.6 2,158,856 - +0.8 2,158,904 - PS417_10055 0.25 -0.3
Or see this region's nucleotide sequence