Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_09475 and PS417_09480 are separated by 49 nucleotides PS417_09480 and PS417_09485 overlap by 8 nucleotides
PS417_09475: PS417_09475 - class V aminotransferase, at 2,051,234 to 2,052,394
_09475
PS417_09480: PS417_09480 - peptidase M24, at 2,052,444 to 2,054,252
_09480
PS417_09485: PS417_09485 - LysR family transcriptional regulator, at 2,054,245 to 2,055,153
_09485
Position (kb)
2052
2053
2054
2055 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2051.603 kb on + strand, within PS417_09475 at 2051.886 kb on - strand, within PS417_09475 at 2052.124 kb on + strand, within PS417_09475 at 2052.124 kb on + strand, within PS417_09475 at 2052.286 kb on + strand at 2052.314 kb on - strand at 2052.314 kb on - strand at 2052.401 kb on + strand at 2052.402 kb on - strand at 2052.413 kb on + strand at 2052.414 kb on - strand at 2052.414 kb on - strand at 2052.416 kb on + strand at 2052.417 kb on - strand at 2052.450 kb on - strand at 2052.568 kb on - strand at 2052.939 kb on + strand, within PS417_09480 at 2052.940 kb on - strand, within PS417_09480 at 2053.476 kb on - strand, within PS417_09480 at 2053.537 kb on + strand, within PS417_09480 at 2053.537 kb on + strand, within PS417_09480 at 2053.538 kb on - strand, within PS417_09480 at 2053.538 kb on - strand, within PS417_09480 at 2053.538 kb on - strand, within PS417_09480 at 2053.566 kb on + strand, within PS417_09480 at 2053.593 kb on + strand, within PS417_09480 at 2053.854 kb on + strand, within PS417_09480 at 2053.855 kb on - strand, within PS417_09480 at 2053.879 kb on + strand, within PS417_09480 at 2054.292 kb on + strand at 2054.292 kb on + strand at 2054.293 kb on - strand at 2054.294 kb on + strand at 2054.294 kb on + strand at 2054.295 kb on - strand at 2054.295 kb on - strand at 2054.375 kb on + strand, within PS417_09485 at 2054.420 kb on + strand, within PS417_09485 at 2054.431 kb on + strand, within PS417_09485 at 2054.432 kb on - strand, within PS417_09485 at 2054.546 kb on + strand, within PS417_09485 at 2054.802 kb on - strand, within PS417_09485 at 2054.957 kb on + strand, within PS417_09485 at 2054.957 kb on + strand, within PS417_09485 at 2054.957 kb on + strand, within PS417_09485 at 2054.960 kb on + strand, within PS417_09485 at 2054.961 kb on - strand, within PS417_09485 at 2054.961 kb on - strand, within PS417_09485 at 2055.046 kb on + strand, within PS417_09485 at 2055.189 kb on + strand at 2055.191 kb on + strand at 2055.249 kb on + strand at 2055.249 kb on + strand at 2055.249 kb on + strand at 2055.249 kb on + strand at 2055.250 kb on - strand at 2055.250 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 2,051,603 + PS417_09475 0.32 +0.8 2,051,886 - PS417_09475 0.56 -1.9 2,052,124 + PS417_09475 0.77 -0.7 2,052,124 + PS417_09475 0.77 +1.2 2,052,286 + +1.3 2,052,314 - -0.8 2,052,314 - +0.1 2,052,401 + -1.5 2,052,402 - +0.7 2,052,413 + +0.6 2,052,414 - -1.6 2,052,414 - -0.0 2,052,416 + +0.2 2,052,417 - +3.7 2,052,450 - +0.5 2,052,568 - -2.1 2,052,939 + PS417_09480 0.27 +0.5 2,052,940 - PS417_09480 0.27 -0.1 2,053,476 - PS417_09480 0.57 -1.6 2,053,537 + PS417_09480 0.60 +0.7 2,053,537 + PS417_09480 0.60 -1.3 2,053,538 - PS417_09480 0.60 +1.6 2,053,538 - PS417_09480 0.60 -1.6 2,053,538 - PS417_09480 0.60 -0.5 2,053,566 + PS417_09480 0.62 +0.6 2,053,593 + PS417_09480 0.64 -0.4 2,053,854 + PS417_09480 0.78 +0.5 2,053,855 - PS417_09480 0.78 -0.7 2,053,879 + PS417_09480 0.79 -3.7 2,054,292 + -2.3 2,054,292 + -1.7 2,054,293 - +1.8 2,054,294 + -2.7 2,054,294 + +0.4 2,054,295 - +1.4 2,054,295 - -1.6 2,054,375 + PS417_09485 0.14 -0.9 2,054,420 + PS417_09485 0.19 -0.0 2,054,431 + PS417_09485 0.20 -1.3 2,054,432 - PS417_09485 0.21 +1.1 2,054,546 + PS417_09485 0.33 -0.0 2,054,802 - PS417_09485 0.61 +1.0 2,054,957 + PS417_09485 0.78 +0.4 2,054,957 + PS417_09485 0.78 +0.3 2,054,957 + PS417_09485 0.78 +0.8 2,054,960 + PS417_09485 0.79 +0.6 2,054,961 - PS417_09485 0.79 +0.5 2,054,961 - PS417_09485 0.79 +0.3 2,055,046 + PS417_09485 0.88 +0.0 2,055,189 + -0.4 2,055,191 + +0.9 2,055,249 + +0.8 2,055,249 + +0.1 2,055,249 + +1.2 2,055,249 + +2.3 2,055,250 - +0.2 2,055,250 - +0.5
Or see this region's nucleotide sequence