Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_09250 and PS417_09255 are separated by 19 nucleotides PS417_09255 and PS417_09260 are separated by 396 nucleotides PS417_09260 and PS417_09265 are separated by 19 nucleotides PS417_09265 and PS417_09270 are separated by 305 nucleotides PS417_09270 and PS417_09275 are separated by 27 nucleotides
PS417_09250: PS417_09250 - tRNA-Val, at 2,009,427 to 2,009,502
_09250
PS417_09255: PS417_09255 - tRNA-Asp, at 2,009,522 to 2,009,598
_09255
PS417_09260: PS417_09260 - tRNA-Val, at 2,009,995 to 2,010,070
_09260
PS417_09265: PS417_09265 - tRNA-Asp, at 2,010,090 to 2,010,166
_09265
PS417_09270: PS417_09270 - BCCT transporter, at 2,010,472 to 2,012,055
_09270
PS417_09275: PS417_09275 - peptidase S15, at 2,012,083 to 2,014,092
_09275
Position (kb)
2010
2011
2012
2013 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2009.742 kb on + strand at 2009.742 kb on + strand at 2009.759 kb on - strand at 2009.789 kb on - strand at 2009.823 kb on + strand at 2009.823 kb on + strand at 2009.823 kb on + strand at 2009.824 kb on - strand at 2009.879 kb on + strand at 2009.880 kb on - strand at 2009.880 kb on - strand at 2010.248 kb on - strand at 2010.373 kb on + strand at 2010.375 kb on + strand at 2010.479 kb on - strand at 2010.545 kb on - strand at 2010.665 kb on - strand, within PS417_09270 at 2010.803 kb on - strand, within PS417_09270 at 2010.811 kb on + strand, within PS417_09270 at 2010.811 kb on + strand, within PS417_09270 at 2010.812 kb on - strand, within PS417_09270 at 2010.952 kb on + strand at 2011.127 kb on - strand, within PS417_09270 at 2011.186 kb on - strand, within PS417_09270 at 2011.186 kb on - strand, within PS417_09270 at 2011.186 kb on - strand, within PS417_09270 at 2011.248 kb on + strand, within PS417_09270 at 2011.303 kb on + strand, within PS417_09270 at 2011.304 kb on - strand, within PS417_09270 at 2011.576 kb on + strand, within PS417_09270 at 2011.645 kb on + strand, within PS417_09270 at 2011.645 kb on + strand, within PS417_09270 at 2012.078 kb on + strand at 2012.078 kb on + strand at 2012.225 kb on - strand at 2012.299 kb on + strand, within PS417_09275 at 2012.299 kb on + strand, within PS417_09275 at 2012.299 kb on + strand at 2012.299 kb on + strand, within PS417_09275 at 2012.299 kb on + strand, within PS417_09275 at 2012.300 kb on - strand, within PS417_09275 at 2012.319 kb on + strand, within PS417_09275 at 2012.320 kb on - strand, within PS417_09275 at 2012.564 kb on - strand, within PS417_09275 at 2012.743 kb on + strand, within PS417_09275 at 2012.744 kb on - strand, within PS417_09275 at 2012.903 kb on - strand, within PS417_09275 at 2013.022 kb on + strand, within PS417_09275 at 2013.055 kb on + strand, within PS417_09275 at 2013.055 kb on + strand, within PS417_09275 at 2013.055 kb on + strand, within PS417_09275
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 2,009,742 + +1.8 2,009,742 + +0.4 2,009,759 - +1.7 2,009,789 - -3.3 2,009,823 + -1.9 2,009,823 + -0.7 2,009,823 + +0.3 2,009,824 - +0.4 2,009,879 + +1.5 2,009,880 - -1.2 2,009,880 - +2.4 2,010,248 - -2.4 2,010,373 + +0.0 2,010,375 + +1.5 2,010,479 - -2.2 2,010,545 - +0.2 2,010,665 - PS417_09270 0.12 -1.5 2,010,803 - PS417_09270 0.21 +2.1 2,010,811 + PS417_09270 0.21 +0.9 2,010,811 + PS417_09270 0.21 -2.5 2,010,812 - PS417_09270 0.21 +0.8 2,010,952 + -0.5 2,011,127 - PS417_09270 0.41 +0.2 2,011,186 - PS417_09270 0.45 +1.0 2,011,186 - PS417_09270 0.45 +0.3 2,011,186 - PS417_09270 0.45 +0.8 2,011,248 + PS417_09270 0.49 +0.5 2,011,303 + PS417_09270 0.52 +0.0 2,011,304 - PS417_09270 0.53 +0.0 2,011,576 + PS417_09270 0.70 +1.6 2,011,645 + PS417_09270 0.74 +0.1 2,011,645 + PS417_09270 0.74 -2.4 2,012,078 + -0.2 2,012,078 + -0.0 2,012,225 - -1.6 2,012,299 + PS417_09275 0.11 -0.9 2,012,299 + PS417_09275 0.11 -2.1 2,012,299 + +2.2 2,012,299 + PS417_09275 0.11 +0.4 2,012,299 + PS417_09275 0.11 +0.8 2,012,300 - PS417_09275 0.11 +0.4 2,012,319 + PS417_09275 0.12 -0.6 2,012,320 - PS417_09275 0.12 +0.5 2,012,564 - PS417_09275 0.24 -0.1 2,012,743 + PS417_09275 0.33 +0.3 2,012,744 - PS417_09275 0.33 +1.0 2,012,903 - PS417_09275 0.41 +0.4 2,013,022 + PS417_09275 0.47 +0.8 2,013,055 + PS417_09275 0.48 -0.8 2,013,055 + PS417_09275 0.48 -3.0 2,013,055 + PS417_09275 0.48 -0.7
Or see this region's nucleotide sequence