Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_09240 and PS417_09245 are separated by 172 nucleotides PS417_09245 and PS417_09250 are separated by 159 nucleotides PS417_09250 and PS417_09255 are separated by 19 nucleotides PS417_09255 and PS417_09260 are separated by 396 nucleotides PS417_09260 and PS417_09265 are separated by 19 nucleotides
PS417_09240: PS417_09240 - sodium transporter, at 2,007,209 to 2,008,174
_09240
PS417_09245: PS417_09245 - recombinase RdgC, at 2,008,347 to 2,009,267
_09245
PS417_09250: PS417_09250 - tRNA-Val, at 2,009,427 to 2,009,502
_09250
PS417_09255: PS417_09255 - tRNA-Asp, at 2,009,522 to 2,009,598
_09255
PS417_09260: PS417_09260 - tRNA-Val, at 2,009,995 to 2,010,070
_09260
PS417_09265: PS417_09265 - tRNA-Asp, at 2,010,090 to 2,010,166
_09265
Position (kb)
2008
2009
2010 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2008.135 kb on + strand at 2008.135 kb on + strand at 2008.136 kb on - strand at 2008.284 kb on - strand at 2008.348 kb on + strand at 2008.348 kb on + strand at 2008.970 kb on - strand at 2009.049 kb on - strand, within PS417_09245 at 2009.049 kb on - strand, within PS417_09245 at 2009.144 kb on - strand, within PS417_09245 at 2009.247 kb on - strand at 2009.247 kb on - strand at 2009.287 kb on + strand at 2009.288 kb on - strand at 2009.389 kb on - strand at 2009.389 kb on - strand at 2009.402 kb on - strand at 2009.742 kb on + strand at 2009.742 kb on + strand at 2009.759 kb on - strand at 2009.789 kb on - strand at 2009.823 kb on + strand at 2009.823 kb on + strand at 2009.823 kb on + strand at 2009.824 kb on - strand at 2009.879 kb on + strand at 2009.880 kb on - strand at 2009.880 kb on - strand at 2010.248 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 2,008,135 + +0.8 2,008,135 + +0.1 2,008,136 - -0.5 2,008,284 - +0.5 2,008,348 + +1.5 2,008,348 + -3.4 2,008,970 - +2.8 2,009,049 - PS417_09245 0.76 +0.3 2,009,049 - PS417_09245 0.76 -2.9 2,009,144 - PS417_09245 0.87 -1.6 2,009,247 - -2.1 2,009,247 - -0.5 2,009,287 + -3.5 2,009,288 - -2.2 2,009,389 - -2.1 2,009,389 - -0.5 2,009,402 - -0.5 2,009,742 + +1.8 2,009,742 + +0.4 2,009,759 - +1.7 2,009,789 - -3.3 2,009,823 + -1.9 2,009,823 + -0.7 2,009,823 + +0.3 2,009,824 - +0.4 2,009,879 + +1.5 2,009,880 - -1.2 2,009,880 - +2.4 2,010,248 - -2.4
Or see this region's nucleotide sequence