Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_09075 and PS417_09080 are separated by 37 nucleotides PS417_09080 and PS417_09085 are separated by 152 nucleotides
PS417_09075: PS417_09075 - chemotaxis protein, at 1,975,615 to 1,977,582
_09075
PS417_09080: PS417_09080 - 2-succinyl-6-hydroxy-2, 4-cyclohexadiene-1-carboxylate synthase, at 1,977,620 to 1,978,432
_09080
PS417_09085: PS417_09085 - hypothetical protein, at 1,978,585 to 1,979,355
_09085
Position (kb)
1977
1978
1979 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1977.066 kb on + strand, within PS417_09075 at 1977.067 kb on - strand, within PS417_09075 at 1977.194 kb on + strand, within PS417_09075 at 1977.498 kb on + strand at 1977.558 kb on + strand at 1977.559 kb on - strand at 1977.688 kb on + strand at 1977.831 kb on - strand, within PS417_09080 at 1977.831 kb on - strand, within PS417_09080 at 1977.831 kb on - strand, within PS417_09080 at 1977.831 kb on - strand, within PS417_09080 at 1977.831 kb on - strand, within PS417_09080 at 1977.898 kb on - strand, within PS417_09080 at 1977.927 kb on + strand at 1977.927 kb on + strand at 1978.038 kb on + strand, within PS417_09080 at 1978.074 kb on + strand, within PS417_09080 at 1978.075 kb on - strand, within PS417_09080 at 1978.075 kb on - strand, within PS417_09080 at 1978.141 kb on + strand, within PS417_09080 at 1978.350 kb on + strand, within PS417_09080 at 1978.350 kb on + strand, within PS417_09080 at 1978.351 kb on - strand at 1978.391 kb on + strand at 1978.456 kb on + strand at 1978.457 kb on - strand at 1978.592 kb on + strand at 1978.768 kb on - strand, within PS417_09085 at 1979.232 kb on + strand, within PS417_09085 at 1979.291 kb on + strand at 1979.291 kb on + strand at 1979.292 kb on - strand at 1979.327 kb on - strand at 1979.372 kb on + strand at 1979.372 kb on + strand at 1979.384 kb on + strand at 1979.391 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 1,977,066 + PS417_09075 0.74 +0.0 1,977,067 - PS417_09075 0.74 -0.4 1,977,194 + PS417_09075 0.80 -2.2 1,977,498 + +0.9 1,977,558 + -0.3 1,977,559 - +1.8 1,977,688 + -2.1 1,977,831 - PS417_09080 0.26 -0.1 1,977,831 - PS417_09080 0.26 +0.5 1,977,831 - PS417_09080 0.26 -0.2 1,977,831 - PS417_09080 0.26 +0.2 1,977,831 - PS417_09080 0.26 +1.8 1,977,898 - PS417_09080 0.34 +0.7 1,977,927 + -0.5 1,977,927 + -0.2 1,978,038 + PS417_09080 0.51 +0.6 1,978,074 + PS417_09080 0.56 +1.8 1,978,075 - PS417_09080 0.56 -0.2 1,978,075 - PS417_09080 0.56 -3.4 1,978,141 + PS417_09080 0.64 +1.0 1,978,350 + PS417_09080 0.90 -0.1 1,978,350 + PS417_09080 0.90 +0.9 1,978,351 - +0.1 1,978,391 + +0.3 1,978,456 + -0.7 1,978,457 - +0.3 1,978,592 + -0.4 1,978,768 - PS417_09085 0.24 -1.6 1,979,232 + PS417_09085 0.84 +1.1 1,979,291 + +0.6 1,979,291 + -1.7 1,979,292 - +0.1 1,979,327 - +1.7 1,979,372 + +1.8 1,979,372 + +0.1 1,979,384 + +0.0 1,979,391 + -0.1
Or see this region's nucleotide sequence