Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_09030 and PS417_09035 are separated by 102 nucleotides PS417_09035 and PS417_09040 are separated by 72 nucleotides PS417_09040 and PS417_09045 are separated by 64 nucleotides
PS417_09030: PS417_09030 - alpha/beta hydrolase, at 1,966,019 to 1,966,873
_09030
PS417_09035: PS417_09035 - hypothetical protein, at 1,966,976 to 1,967,794
_09035
PS417_09040: PS417_09040 - hypothetical protein, at 1,967,867 to 1,968,928
_09040
PS417_09045: PS417_09045 - histidine kinase, at 1,968,993 to 1,970,024
_09045
Position (kb)
1967
1968
1969 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1966.975 kb on + strand at 1966.975 kb on + strand at 1966.976 kb on - strand at 1966.997 kb on - strand at 1967.134 kb on + strand, within PS417_09035 at 1967.135 kb on - strand, within PS417_09035 at 1967.138 kb on + strand, within PS417_09035 at 1967.226 kb on - strand, within PS417_09035 at 1967.496 kb on + strand, within PS417_09035 at 1967.798 kb on + strand at 1967.830 kb on - strand at 1967.842 kb on + strand at 1967.842 kb on + strand at 1967.864 kb on + strand at 1967.864 kb on + strand at 1967.865 kb on - strand at 1967.865 kb on - strand at 1967.875 kb on + strand at 1968.182 kb on - strand, within PS417_09040 at 1968.281 kb on + strand, within PS417_09040 at 1968.452 kb on + strand, within PS417_09040 at 1968.452 kb on + strand, within PS417_09040 at 1968.453 kb on - strand, within PS417_09040 at 1968.455 kb on + strand, within PS417_09040 at 1968.671 kb on + strand, within PS417_09040 at 1968.671 kb on + strand, within PS417_09040 at 1968.672 kb on - strand, within PS417_09040 at 1968.678 kb on - strand, within PS417_09040 at 1968.863 kb on + strand at 1968.863 kb on + strand at 1969.327 kb on - strand, within PS417_09045 at 1969.454 kb on - strand, within PS417_09045 at 1969.454 kb on - strand, within PS417_09045 at 1969.647 kb on - strand, within PS417_09045 at 1969.777 kb on + strand, within PS417_09045
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 1,966,975 + -2.9 1,966,975 + +1.2 1,966,976 - -2.7 1,966,997 - -3.1 1,967,134 + PS417_09035 0.19 +2.1 1,967,135 - PS417_09035 0.19 -0.1 1,967,138 + PS417_09035 0.20 -0.7 1,967,226 - PS417_09035 0.31 -1.5 1,967,496 + PS417_09035 0.63 +1.2 1,967,798 + -0.6 1,967,830 - -0.3 1,967,842 + +0.4 1,967,842 + +0.8 1,967,864 + +2.3 1,967,864 + +0.8 1,967,865 - -0.4 1,967,865 - -0.2 1,967,875 + -3.1 1,968,182 - PS417_09040 0.30 -0.0 1,968,281 + PS417_09040 0.39 +0.1 1,968,452 + PS417_09040 0.55 +1.1 1,968,452 + PS417_09040 0.55 +0.9 1,968,453 - PS417_09040 0.55 +0.5 1,968,455 + PS417_09040 0.55 -2.4 1,968,671 + PS417_09040 0.76 +1.0 1,968,671 + PS417_09040 0.76 -1.3 1,968,672 - PS417_09040 0.76 -1.3 1,968,678 - PS417_09040 0.76 +1.8 1,968,863 + -0.5 1,968,863 + +0.3 1,969,327 - PS417_09045 0.32 -3.0 1,969,454 - PS417_09045 0.45 -1.9 1,969,454 - PS417_09045 0.45 -2.7 1,969,647 - PS417_09045 0.63 -2.7 1,969,777 + PS417_09045 0.76 +1.2
Or see this region's nucleotide sequence