Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_08855 and PS417_08860 are separated by 193 nucleotides PS417_08860 and PS417_08865 overlap by 4 nucleotides
PS417_08855: PS417_08855 - glycine/betaine ABC transporter substrate-binding protein, at 1,926,185 to 1,927,036
_08855
PS417_08860: PS417_08860 - small component of pyruvate transporter (yjcH-like) (from data) , at 1,927,230 to 1,927,541
_08860
PS417_08865: PS417_08865 - large component of pyruvate transporter (actP-like) (from data) , at 1,927,538 to 1,929,196
_08865
Position (kb)
1927
1928 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1926.413 kb on - strand, within PS417_08855 at 1926.543 kb on - strand, within PS417_08855 at 1926.660 kb on - strand, within PS417_08855 at 1926.672 kb on - strand, within PS417_08855 at 1926.821 kb on + strand, within PS417_08855 at 1926.821 kb on + strand, within PS417_08855 at 1926.821 kb on + strand, within PS417_08855 at 1927.034 kb on + strand at 1927.129 kb on + strand at 1927.140 kb on + strand at 1927.141 kb on - strand at 1927.141 kb on - strand at 1927.143 kb on + strand at 1927.144 kb on - strand at 1927.144 kb on - strand at 1927.146 kb on + strand at 1927.146 kb on + strand at 1927.146 kb on + strand at 1927.147 kb on - strand at 1927.147 kb on - strand at 1927.147 kb on - strand at 1927.179 kb on - strand at 1927.179 kb on - strand at 1927.179 kb on - strand at 1927.179 kb on - strand at 1927.187 kb on + strand at 1927.245 kb on + strand at 1927.245 kb on + strand at 1927.473 kb on + strand, within PS417_08860 at 1927.473 kb on + strand, within PS417_08860 at 1927.473 kb on + strand, within PS417_08860 at 1927.473 kb on + strand, within PS417_08860 at 1927.473 kb on + strand, within PS417_08860 at 1927.474 kb on - strand, within PS417_08860 at 1927.474 kb on - strand, within PS417_08860 at 1927.474 kb on - strand, within PS417_08860 at 1927.474 kb on - strand at 1927.474 kb on - strand at 1927.474 kb on - strand, within PS417_08860 at 1927.474 kb on - strand, within PS417_08860 at 1927.694 kb on + strand at 1927.695 kb on - strand at 1927.695 kb on - strand at 1927.730 kb on + strand, within PS417_08865 at 1927.731 kb on - strand, within PS417_08865 at 1927.790 kb on + strand, within PS417_08865 at 1927.790 kb on + strand, within PS417_08865 at 1927.791 kb on - strand, within PS417_08865 at 1927.791 kb on - strand, within PS417_08865 at 1927.791 kb on - strand, within PS417_08865 at 1927.791 kb on - strand, within PS417_08865 at 1927.791 kb on - strand, within PS417_08865 at 1927.791 kb on - strand, within PS417_08865 at 1927.833 kb on - strand, within PS417_08865 at 1927.833 kb on - strand, within PS417_08865 at 1927.859 kb on + strand, within PS417_08865 at 1927.860 kb on - strand, within PS417_08865 at 1927.922 kb on - strand at 1927.934 kb on + strand, within PS417_08865 at 1927.934 kb on + strand at 1927.935 kb on - strand, within PS417_08865 at 1927.935 kb on - strand, within PS417_08865 at 1927.959 kb on - strand, within PS417_08865 at 1928.025 kb on - strand, within PS417_08865 at 1928.084 kb on + strand, within PS417_08865 at 1928.084 kb on + strand, within PS417_08865 at 1928.085 kb on - strand, within PS417_08865 at 1928.091 kb on + strand, within PS417_08865 at 1928.091 kb on + strand, within PS417_08865 at 1928.092 kb on - strand, within PS417_08865 at 1928.092 kb on - strand, within PS417_08865 at 1928.092 kb on - strand, within PS417_08865 at 1928.092 kb on - strand, within PS417_08865 at 1928.092 kb on - strand, within PS417_08865 at 1928.092 kb on - strand, within PS417_08865 at 1928.126 kb on + strand, within PS417_08865 at 1928.126 kb on + strand, within PS417_08865 at 1928.127 kb on - strand at 1928.127 kb on - strand, within PS417_08865 at 1928.127 kb on - strand, within PS417_08865 at 1928.468 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 1,926,413 - PS417_08855 0.27 -2.7 1,926,543 - PS417_08855 0.42 +1.3 1,926,660 - PS417_08855 0.56 -0.2 1,926,672 - PS417_08855 0.57 +1.8 1,926,821 + PS417_08855 0.75 -0.7 1,926,821 + PS417_08855 0.75 +1.1 1,926,821 + PS417_08855 0.75 -1.5 1,927,034 + -1.1 1,927,129 + -0.5 1,927,140 + -0.8 1,927,141 - +1.2 1,927,141 - -1.1 1,927,143 + -0.5 1,927,144 - -2.0 1,927,144 - -2.3 1,927,146 + +0.9 1,927,146 + +1.0 1,927,146 + +0.6 1,927,147 - -1.5 1,927,147 - +0.6 1,927,147 - +1.5 1,927,179 - -2.2 1,927,179 - +0.3 1,927,179 - +0.9 1,927,179 - +3.6 1,927,187 + +0.1 1,927,245 + -1.1 1,927,245 + +0.8 1,927,473 + PS417_08860 0.78 +1.2 1,927,473 + PS417_08860 0.78 -3.3 1,927,473 + PS417_08860 0.78 -0.1 1,927,473 + PS417_08860 0.78 -0.1 1,927,473 + PS417_08860 0.78 -1.8 1,927,474 - PS417_08860 0.78 -1.5 1,927,474 - PS417_08860 0.78 -2.1 1,927,474 - PS417_08860 0.78 -1.3 1,927,474 - -0.5 1,927,474 - +0.0 1,927,474 - PS417_08860 0.78 -3.1 1,927,474 - PS417_08860 0.78 -0.1 1,927,694 + -0.5 1,927,695 - +0.4 1,927,695 - +0.4 1,927,730 + PS417_08865 0.12 +1.9 1,927,731 - PS417_08865 0.12 -2.2 1,927,790 + PS417_08865 0.15 +0.5 1,927,790 + PS417_08865 0.15 -1.0 1,927,791 - PS417_08865 0.15 +0.4 1,927,791 - PS417_08865 0.15 -3.4 1,927,791 - PS417_08865 0.15 +0.2 1,927,791 - PS417_08865 0.15 -0.7 1,927,791 - PS417_08865 0.15 +0.7 1,927,791 - PS417_08865 0.15 -0.1 1,927,833 - PS417_08865 0.18 +0.9 1,927,833 - PS417_08865 0.18 +0.4 1,927,859 + PS417_08865 0.19 +0.7 1,927,860 - PS417_08865 0.19 -2.1 1,927,922 - -1.7 1,927,934 + PS417_08865 0.24 -0.6 1,927,934 + +2.1 1,927,935 - PS417_08865 0.24 -0.0 1,927,935 - PS417_08865 0.24 -0.5 1,927,959 - PS417_08865 0.25 -1.7 1,928,025 - PS417_08865 0.29 +0.7 1,928,084 + PS417_08865 0.33 +0.3 1,928,084 + PS417_08865 0.33 -2.0 1,928,085 - PS417_08865 0.33 +0.6 1,928,091 + PS417_08865 0.33 -1.3 1,928,091 + PS417_08865 0.33 +1.9 1,928,092 - PS417_08865 0.33 -0.8 1,928,092 - PS417_08865 0.33 +0.0 1,928,092 - PS417_08865 0.33 +0.6 1,928,092 - PS417_08865 0.33 -2.7 1,928,092 - PS417_08865 0.33 +2.9 1,928,092 - PS417_08865 0.33 +1.6 1,928,126 + PS417_08865 0.35 -0.5 1,928,126 + PS417_08865 0.35 -0.3 1,928,127 - -0.5 1,928,127 - PS417_08865 0.36 -1.1 1,928,127 - PS417_08865 0.36 +1.5 1,928,468 + -0.5
Or see this region's nucleotide sequence