Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_00840 and PS417_00845 are separated by 76 nucleotides PS417_00845 and PS417_00850 are separated by 69 nucleotides PS417_00850 and PS417_00855 overlap by 38 nucleotides PS417_00855 and PS417_00860 are separated by 92 nucleotides
PS417_00840: PS417_00840 - hypothetical protein, at 186,254 to 190,063
_00840
PS417_00845: PS417_00845 - transcriptional regulator, at 190,140 to 190,505
_00845
PS417_00850: PS417_00850 - hypothetical protein, at 190,575 to 190,763
_00850
PS417_00855: PS417_00855 - chromosome segregation protein SMC, at 190,726 to 191,670
_00855
PS417_00860: PS417_00860 - hypothetical protein, at 191,763 to 194,669
_00860
Position (kb) 190
191
192 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 189.762 kb on - strand at 189.762 kb on - strand at 189.854 kb on - strand at 189.921 kb on + strand at 189.922 kb on - strand at 190.010 kb on + strand at 190.011 kb on - strand at 190.142 kb on - strand at 190.366 kb on + strand, within PS417_00845 at 190.437 kb on + strand, within PS417_00845 at 190.531 kb on - strand at 190.577 kb on - strand at 190.856 kb on + strand, within PS417_00855 at 190.942 kb on + strand, within PS417_00855 at 190.990 kb on + strand, within PS417_00855 at 191.076 kb on + strand, within PS417_00855 at 191.102 kb on + strand, within PS417_00855 at 191.102 kb on + strand, within PS417_00855 at 191.102 kb on + strand, within PS417_00855 at 191.102 kb on + strand, within PS417_00855 at 191.106 kb on + strand, within PS417_00855 at 191.110 kb on + strand, within PS417_00855 at 191.110 kb on + strand, within PS417_00855 at 191.163 kb on + strand, within PS417_00855 at 191.164 kb on - strand, within PS417_00855 at 191.470 kb on + strand, within PS417_00855 at 191.470 kb on + strand, within PS417_00855 at 191.470 kb on + strand, within PS417_00855 at 191.470 kb on + strand, within PS417_00855 at 191.474 kb on - strand, within PS417_00855 at 191.608 kb on + strand at 191.676 kb on + strand at 191.677 kb on - strand at 191.807 kb on + strand at 191.815 kb on + strand at 191.815 kb on + strand at 191.815 kb on + strand at 191.816 kb on - strand at 191.886 kb on + strand at 191.886 kb on + strand at 191.886 kb on + strand at 191.971 kb on + strand at 191.971 kb on + strand at 191.974 kb on + strand at 191.975 kb on - strand at 191.975 kb on - strand at 192.449 kb on - strand, within PS417_00860 at 192.564 kb on + strand, within PS417_00860
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 189,762 - +0.2 189,762 - -0.4 189,854 - -0.3 189,921 + -1.4 189,922 - -0.4 190,010 + -1.0 190,011 - +0.3 190,142 - -0.5 190,366 + PS417_00845 0.62 +0.7 190,437 + PS417_00845 0.81 -1.4 190,531 - -0.8 190,577 - +0.8 190,856 + PS417_00855 0.14 +2.6 190,942 + PS417_00855 0.23 -1.0 190,990 + PS417_00855 0.28 -1.2 191,076 + PS417_00855 0.37 +0.8 191,102 + PS417_00855 0.40 -1.2 191,102 + PS417_00855 0.40 +0.7 191,102 + PS417_00855 0.40 -0.8 191,102 + PS417_00855 0.40 -0.1 191,106 + PS417_00855 0.40 +1.4 191,110 + PS417_00855 0.41 +0.4 191,110 + PS417_00855 0.41 -1.4 191,163 + PS417_00855 0.46 +0.6 191,164 - PS417_00855 0.46 -3.4 191,470 + PS417_00855 0.79 -1.1 191,470 + PS417_00855 0.79 +0.4 191,470 + PS417_00855 0.79 +2.0 191,470 + PS417_00855 0.79 -0.6 191,474 - PS417_00855 0.79 +3.4 191,608 + -3.7 191,676 + -0.3 191,677 - -3.4 191,807 + +0.3 191,815 + -0.4 191,815 + -1.4 191,815 + +1.4 191,816 - -2.1 191,886 + +2.3 191,886 + -0.5 191,886 + +0.9 191,971 + -1.1 191,971 + -2.5 191,974 + +0.4 191,975 - -4.0 191,975 - -1.0 192,449 - PS417_00860 0.24 -1.1 192,564 + PS417_00860 0.28 +2.0
Or see this region's nucleotide sequence