Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_08385 and PS417_08390 are separated by 113 nucleotides PS417_08390 and PS417_08395 are separated by 45 nucleotides PS417_08395 and PS417_08400 overlap by 1 nucleotides PS417_08400 and PS417_08405 are separated by 10 nucleotides
PS417_08385: PS417_08385 - hypothetical protein, at 1,838,518 to 1,839,489
_08385
PS417_08390: PS417_08390 - transcription elongation factor GreB, at 1,839,603 to 1,840,076
_08390
PS417_08395: PS417_08395 - ABC transporter permease, at 1,840,122 to 1,842,632
_08395
PS417_08400: PS417_08400 - ABC transporter, at 1,842,632 to 1,843,315
_08400
PS417_08405: PS417_08405 - esterase, at 1,843,326 to 1,843,931
_08405
Position (kb)
1840
1841
1842
1843 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1839.180 kb on + strand, within PS417_08385 at 1839.181 kb on - strand, within PS417_08385 at 1839.181 kb on - strand, within PS417_08385 at 1839.181 kb on - strand, within PS417_08385 at 1839.456 kb on - strand at 1839.488 kb on - strand at 1839.488 kb on - strand at 1839.502 kb on - strand at 1839.502 kb on - strand at 1839.519 kb on + strand at 1839.575 kb on + strand at 1839.576 kb on - strand at 1839.576 kb on - strand at 1839.576 kb on - strand at 1839.660 kb on + strand, within PS417_08390 at 1839.660 kb on + strand, within PS417_08390 at 1839.831 kb on + strand, within PS417_08390 at 1839.831 kb on + strand, within PS417_08390 at 1839.831 kb on + strand, within PS417_08390 at 1839.832 kb on - strand, within PS417_08390 at 1839.832 kb on - strand, within PS417_08390 at 1839.925 kb on - strand, within PS417_08390 at 1839.936 kb on + strand, within PS417_08390 at 1839.951 kb on + strand, within PS417_08390 at 1839.951 kb on + strand, within PS417_08390 at 1839.951 kb on + strand, within PS417_08390 at 1840.000 kb on + strand, within PS417_08390 at 1840.066 kb on - strand at 1840.098 kb on + strand at 1840.200 kb on + strand at 1840.264 kb on + strand at 1840.264 kb on + strand at 1840.486 kb on + strand, within PS417_08395 at 1840.486 kb on + strand, within PS417_08395 at 1840.486 kb on + strand, within PS417_08395 at 1840.487 kb on - strand, within PS417_08395 at 1840.487 kb on - strand, within PS417_08395 at 1840.618 kb on + strand, within PS417_08395 at 1840.633 kb on + strand, within PS417_08395 at 1840.633 kb on + strand, within PS417_08395 at 1840.634 kb on - strand, within PS417_08395 at 1840.634 kb on - strand, within PS417_08395 at 1840.982 kb on + strand, within PS417_08395 at 1841.425 kb on + strand, within PS417_08395 at 1841.617 kb on + strand, within PS417_08395 at 1841.618 kb on - strand, within PS417_08395 at 1841.618 kb on - strand, within PS417_08395 at 1841.947 kb on + strand, within PS417_08395 at 1842.499 kb on + strand at 1842.499 kb on + strand at 1842.499 kb on + strand at 1842.500 kb on - strand at 1843.283 kb on - strand at 1843.391 kb on + strand, within PS417_08405 at 1843.413 kb on - strand, within PS417_08405 at 1843.415 kb on + strand, within PS417_08405 at 1843.415 kb on + strand, within PS417_08405 at 1843.415 kb on + strand, within PS417_08405 at 1843.416 kb on - strand, within PS417_08405 at 1843.577 kb on + strand, within PS417_08405 at 1843.577 kb on + strand, within PS417_08405 at 1843.577 kb on + strand, within PS417_08405 at 1843.578 kb on - strand, within PS417_08405 at 1843.578 kb on - strand, within PS417_08405
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 1,839,180 + PS417_08385 0.68 +1.2 1,839,181 - PS417_08385 0.68 +0.2 1,839,181 - PS417_08385 0.68 -1.3 1,839,181 - PS417_08385 0.68 +0.7 1,839,456 - +1.0 1,839,488 - -0.3 1,839,488 - -0.5 1,839,502 - +0.4 1,839,502 - +0.5 1,839,519 + -0.9 1,839,575 + -1.7 1,839,576 - +0.4 1,839,576 - +1.7 1,839,576 - +0.3 1,839,660 + PS417_08390 0.12 +0.8 1,839,660 + PS417_08390 0.12 -3.6 1,839,831 + PS417_08390 0.48 -0.5 1,839,831 + PS417_08390 0.48 +1.3 1,839,831 + PS417_08390 0.48 +0.1 1,839,832 - PS417_08390 0.48 -0.5 1,839,832 - PS417_08390 0.48 -0.3 1,839,925 - PS417_08390 0.68 -1.2 1,839,936 + PS417_08390 0.70 -0.2 1,839,951 + PS417_08390 0.73 -0.8 1,839,951 + PS417_08390 0.73 +0.9 1,839,951 + PS417_08390 0.73 -0.7 1,840,000 + PS417_08390 0.84 +0.2 1,840,066 - -1.7 1,840,098 + +0.9 1,840,200 + -0.2 1,840,264 + +0.8 1,840,264 + -1.8 1,840,486 + PS417_08395 0.14 -1.6 1,840,486 + PS417_08395 0.14 -2.7 1,840,486 + PS417_08395 0.14 -0.7 1,840,487 - PS417_08395 0.15 +0.4 1,840,487 - PS417_08395 0.15 -1.6 1,840,618 + PS417_08395 0.20 +0.1 1,840,633 + PS417_08395 0.20 -2.1 1,840,633 + PS417_08395 0.20 -2.0 1,840,634 - PS417_08395 0.20 +0.3 1,840,634 - PS417_08395 0.20 -1.8 1,840,982 + PS417_08395 0.34 +0.7 1,841,425 + PS417_08395 0.52 -2.0 1,841,617 + PS417_08395 0.60 -0.1 1,841,618 - PS417_08395 0.60 +4.5 1,841,618 - PS417_08395 0.60 -1.3 1,841,947 + PS417_08395 0.73 -0.4 1,842,499 + +0.5 1,842,499 + -3.6 1,842,499 + +0.7 1,842,500 - +0.2 1,843,283 - +0.2 1,843,391 + PS417_08405 0.11 -0.2 1,843,413 - PS417_08405 0.14 -0.3 1,843,415 + PS417_08405 0.15 +0.3 1,843,415 + PS417_08405 0.15 -0.8 1,843,415 + PS417_08405 0.15 +0.9 1,843,416 - PS417_08405 0.15 -0.1 1,843,577 + PS417_08405 0.41 -0.8 1,843,577 + PS417_08405 0.41 +0.2 1,843,577 + PS417_08405 0.41 -1.5 1,843,578 - PS417_08405 0.42 -0.1 1,843,578 - PS417_08405 0.42 -0.3
Or see this region's nucleotide sequence