Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_08315 and PS417_08320 are separated by 44 nucleotides PS417_08320 and PS417_08325 are separated by 90 nucleotides PS417_08325 and PS417_08330 are separated by 2 nucleotides PS417_08330 and PS417_08335 are separated by 201 nucleotides PS417_08335 and PS417_08340 are separated by 19 nucleotides
PS417_08315: PS417_08315 - hypothetical protein, at 1,822,000 to 1,822,278
_08315
PS417_08320: PS417_08320 - metal-chelation protein CHAD, at 1,822,323 to 1,823,084
_08320
PS417_08325: PS417_08325 - acyl-CoA thioesterase, at 1,823,175 to 1,823,972
_08325
PS417_08330: PS417_08330 - terminase, at 1,823,975 to 1,824,337
_08330
PS417_08335: PS417_08335 - hypothetical protein, at 1,824,539 to 1,824,826
_08335
PS417_08340: PS417_08340 - type IV secretion protein Rhs, at 1,824,846 to 1,829,564
_08340
Position (kb)
1823
1824 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1822.249 kb on + strand at 1822.249 kb on + strand at 1822.249 kb on + strand at 1822.249 kb on + strand, within PS417_08315 at 1822.250 kb on - strand, within PS417_08315 at 1822.250 kb on - strand, within PS417_08315 at 1822.250 kb on - strand, within PS417_08315 at 1822.252 kb on - strand at 1822.372 kb on + strand at 1822.372 kb on + strand at 1822.373 kb on - strand at 1822.379 kb on - strand at 1822.573 kb on - strand, within PS417_08320 at 1822.883 kb on + strand, within PS417_08320 at 1822.883 kb on + strand, within PS417_08320 at 1822.883 kb on + strand, within PS417_08320 at 1823.108 kb on + strand at 1823.137 kb on + strand at 1823.183 kb on + strand at 1823.385 kb on + strand, within PS417_08325 at 1823.385 kb on + strand, within PS417_08325 at 1823.470 kb on + strand, within PS417_08325 at 1823.470 kb on + strand, within PS417_08325 at 1823.470 kb on + strand, within PS417_08325 at 1823.471 kb on - strand, within PS417_08325 at 1823.471 kb on - strand, within PS417_08325 at 1823.568 kb on + strand at 1823.569 kb on - strand, within PS417_08325 at 1823.569 kb on - strand, within PS417_08325 at 1823.648 kb on + strand, within PS417_08325 at 1823.720 kb on + strand, within PS417_08325 at 1824.020 kb on + strand, within PS417_08330 at 1824.020 kb on + strand, within PS417_08330 at 1824.021 kb on - strand, within PS417_08330 at 1824.119 kb on - strand, within PS417_08330 at 1824.291 kb on + strand, within PS417_08330 at 1824.295 kb on + strand, within PS417_08330 at 1824.295 kb on + strand, within PS417_08330 at 1824.380 kb on + strand at 1824.380 kb on + strand at 1824.380 kb on + strand at 1824.381 kb on - strand at 1824.428 kb on + strand at 1824.509 kb on + strand at 1824.509 kb on + strand at 1824.547 kb on - strand at 1824.551 kb on + strand at 1824.552 kb on - strand at 1824.552 kb on - strand at 1824.618 kb on + strand, within PS417_08335 at 1824.618 kb on + strand at 1824.619 kb on - strand, within PS417_08335 at 1824.636 kb on - strand, within PS417_08335 at 1824.718 kb on - strand, within PS417_08335 at 1824.857 kb on - strand at 1824.891 kb on + strand at 1824.892 kb on - strand at 1824.892 kb on - strand at 1824.892 kb on - strand at 1824.892 kb on - strand at 1824.937 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 1,822,249 + -1.1 1,822,249 + -1.1 1,822,249 + -0.1 1,822,249 + PS417_08315 0.89 -0.8 1,822,250 - PS417_08315 0.90 -1.2 1,822,250 - PS417_08315 0.90 +0.5 1,822,250 - PS417_08315 0.90 +0.6 1,822,252 - -0.7 1,822,372 + -1.3 1,822,372 + -0.3 1,822,373 - +0.5 1,822,379 - +0.5 1,822,573 - PS417_08320 0.33 -0.7 1,822,883 + PS417_08320 0.73 +0.9 1,822,883 + PS417_08320 0.73 +2.4 1,822,883 + PS417_08320 0.73 +0.6 1,823,108 + +1.9 1,823,137 + -0.5 1,823,183 + +0.3 1,823,385 + PS417_08325 0.26 -0.3 1,823,385 + PS417_08325 0.26 -1.3 1,823,470 + PS417_08325 0.37 +0.4 1,823,470 + PS417_08325 0.37 -0.0 1,823,470 + PS417_08325 0.37 +1.9 1,823,471 - PS417_08325 0.37 +0.7 1,823,471 - PS417_08325 0.37 -0.4 1,823,568 + +1.9 1,823,569 - PS417_08325 0.49 +3.7 1,823,569 - PS417_08325 0.49 -0.0 1,823,648 + PS417_08325 0.59 +0.6 1,823,720 + PS417_08325 0.68 +1.1 1,824,020 + PS417_08330 0.12 +0.3 1,824,020 + PS417_08330 0.12 -2.8 1,824,021 - PS417_08330 0.13 -0.5 1,824,119 - PS417_08330 0.40 +1.0 1,824,291 + PS417_08330 0.87 -0.6 1,824,295 + PS417_08330 0.88 -3.6 1,824,295 + PS417_08330 0.88 +0.7 1,824,380 + -0.2 1,824,380 + -3.3 1,824,380 + -0.0 1,824,381 - +1.1 1,824,428 + -1.0 1,824,509 + -0.1 1,824,509 + -1.1 1,824,547 - -0.3 1,824,551 + +1.5 1,824,552 - +0.1 1,824,552 - -0.7 1,824,618 + PS417_08335 0.27 +0.1 1,824,618 + -0.1 1,824,619 - PS417_08335 0.28 -0.8 1,824,636 - PS417_08335 0.34 -0.9 1,824,718 - PS417_08335 0.62 -2.8 1,824,857 - +0.3 1,824,891 + +0.1 1,824,892 - -1.0 1,824,892 - -0.2 1,824,892 - -0.2 1,824,892 - -0.2 1,824,937 - +0.5
Or see this region's nucleotide sequence