Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_00750 and PS417_00755 are separated by 2 nucleotides PS417_00755 and PS417_00760 are separated by 367 nucleotides PS417_00760 and PS417_00765 are separated by 31 nucleotides
PS417_00750: PS417_00750 - N-glycosyltransferase, at 159,282 to 160,622
_00750
PS417_00755: PS417_00755 - biofilm PGA synthesis protein PgaD, at 160,625 to 161,107
_00755
PS417_00760: PS417_00760 - ShlB family hemolysin secretion/activation protein, at 161,475 to 163,196
_00760
PS417_00765: PS417_00765 - filamentous hemagglutinin, at 163,228 to 170,496
_00765
Position (kb) 161
162
163
164 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 160.651 kb on + strand at 160.651 kb on + strand at 160.651 kb on + strand at 160.652 kb on - strand at 160.652 kb on - strand at 160.652 kb on - strand at 160.805 kb on + strand, within PS417_00755 at 161.106 kb on - strand at 161.157 kb on + strand at 161.221 kb on + strand at 161.222 kb on - strand at 161.261 kb on - strand at 161.344 kb on - strand at 161.344 kb on - strand at 161.434 kb on + strand at 161.779 kb on - strand, within PS417_00760 at 161.779 kb on - strand, within PS417_00760 at 161.874 kb on + strand, within PS417_00760 at 161.874 kb on + strand, within PS417_00760 at 162.262 kb on + strand, within PS417_00760 at 162.324 kb on + strand, within PS417_00760 at 162.325 kb on - strand, within PS417_00760 at 162.327 kb on + strand, within PS417_00760 at 162.327 kb on + strand, within PS417_00760 at 162.360 kb on + strand, within PS417_00760 at 162.366 kb on + strand, within PS417_00760 at 162.366 kb on + strand at 162.367 kb on - strand, within PS417_00760 at 162.390 kb on + strand, within PS417_00760 at 162.390 kb on + strand, within PS417_00760 at 162.390 kb on + strand, within PS417_00760 at 162.390 kb on + strand, within PS417_00760 at 162.390 kb on + strand, within PS417_00760 at 162.391 kb on - strand, within PS417_00760 at 162.391 kb on - strand, within PS417_00760 at 162.391 kb on - strand, within PS417_00760 at 162.524 kb on + strand, within PS417_00760 at 162.524 kb on + strand, within PS417_00760 at 162.525 kb on - strand, within PS417_00760 at 162.525 kb on - strand, within PS417_00760 at 162.525 kb on - strand, within PS417_00760 at 162.527 kb on + strand, within PS417_00760 at 162.527 kb on + strand, within PS417_00760 at 162.527 kb on + strand, within PS417_00760 at 162.528 kb on - strand, within PS417_00760 at 162.528 kb on - strand, within PS417_00760 at 162.528 kb on - strand, within PS417_00760 at 162.528 kb on - strand, within PS417_00760 at 162.528 kb on - strand, within PS417_00760 at 162.531 kb on + strand, within PS417_00760 at 162.531 kb on + strand, within PS417_00760 at 162.531 kb on + strand, within PS417_00760 at 162.532 kb on - strand, within PS417_00760 at 162.532 kb on - strand, within PS417_00760 at 162.532 kb on - strand, within PS417_00760 at 162.532 kb on - strand, within PS417_00760 at 162.532 kb on - strand, within PS417_00760 at 162.532 kb on - strand, within PS417_00760 at 162.548 kb on + strand, within PS417_00760 at 162.732 kb on + strand, within PS417_00760 at 162.733 kb on - strand, within PS417_00760 at 162.750 kb on + strand, within PS417_00760 at 162.751 kb on - strand, within PS417_00760 at 163.033 kb on - strand at 163.034 kb on + strand at 163.170 kb on + strand at 163.171 kb on - strand at 163.194 kb on + strand at 163.328 kb on + strand at 163.329 kb on - strand at 163.489 kb on + strand at 163.561 kb on + strand at 163.561 kb on + strand at 163.562 kb on - strand at 163.564 kb on - strand at 163.799 kb on - strand at 164.034 kb on + strand, within PS417_00765 at 164.034 kb on + strand, within PS417_00765 at 164.038 kb on + strand, within PS417_00765 at 164.038 kb on + strand, within PS417_00765 at 164.038 kb on + strand, within PS417_00765 at 164.039 kb on - strand, within PS417_00765 at 164.039 kb on - strand, within PS417_00765
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 160,651 + -2.1 160,651 + +1.5 160,651 + -2.2 160,652 - +1.0 160,652 - +0.4 160,652 - +0.4 160,805 + PS417_00755 0.37 -0.5 161,106 - -0.3 161,157 + +1.6 161,221 + +0.8 161,222 - -1.0 161,261 - -1.2 161,344 - -1.7 161,344 - -2.2 161,434 + +0.5 161,779 - PS417_00760 0.18 +0.2 161,779 - PS417_00760 0.18 -0.5 161,874 + PS417_00760 0.23 -1.0 161,874 + PS417_00760 0.23 +0.6 162,262 + PS417_00760 0.46 +0.0 162,324 + PS417_00760 0.49 +0.6 162,325 - PS417_00760 0.49 +0.2 162,327 + PS417_00760 0.49 -2.9 162,327 + PS417_00760 0.49 +0.9 162,360 + PS417_00760 0.51 +0.3 162,366 + PS417_00760 0.52 +1.2 162,366 + -0.2 162,367 - PS417_00760 0.52 +1.1 162,390 + PS417_00760 0.53 -0.7 162,390 + PS417_00760 0.53 +1.0 162,390 + PS417_00760 0.53 -0.1 162,390 + PS417_00760 0.53 -0.1 162,390 + PS417_00760 0.53 -0.7 162,391 - PS417_00760 0.53 -0.3 162,391 - PS417_00760 0.53 -1.2 162,391 - PS417_00760 0.53 +0.8 162,524 + PS417_00760 0.61 +0.4 162,524 + PS417_00760 0.61 +0.8 162,525 - PS417_00760 0.61 -1.1 162,525 - PS417_00760 0.61 -0.6 162,525 - PS417_00760 0.61 +0.8 162,527 + PS417_00760 0.61 +0.5 162,527 + PS417_00760 0.61 +0.4 162,527 + PS417_00760 0.61 +0.3 162,528 - PS417_00760 0.61 +0.2 162,528 - PS417_00760 0.61 -1.0 162,528 - PS417_00760 0.61 -1.8 162,528 - PS417_00760 0.61 +2.2 162,528 - PS417_00760 0.61 +3.2 162,531 + PS417_00760 0.61 +0.1 162,531 + PS417_00760 0.61 -0.8 162,531 + PS417_00760 0.61 -2.4 162,532 - PS417_00760 0.61 -0.8 162,532 - PS417_00760 0.61 +0.1 162,532 - PS417_00760 0.61 -1.5 162,532 - PS417_00760 0.61 +0.0 162,532 - PS417_00760 0.61 +5.5 162,532 - PS417_00760 0.61 -0.1 162,548 + PS417_00760 0.62 +3.6 162,732 + PS417_00760 0.73 +0.8 162,733 - PS417_00760 0.73 -2.5 162,750 + PS417_00760 0.74 +0.5 162,751 - PS417_00760 0.74 -0.3 163,033 - +1.4 163,034 + +0.7 163,170 + +0.3 163,171 - -0.5 163,194 + -2.3 163,328 + -0.6 163,329 - -0.5 163,489 + -0.7 163,561 + -0.6 163,561 + -0.8 163,562 - +0.7 163,564 - -0.1 163,799 - +0.9 164,034 + PS417_00765 0.11 -1.5 164,034 + PS417_00765 0.11 +1.4 164,038 + PS417_00765 0.11 +0.4 164,038 + PS417_00765 0.11 -1.7 164,038 + PS417_00765 0.11 +0.5 164,039 - PS417_00765 0.11 +1.6 164,039 - PS417_00765 0.11 +1.2
Or see this region's nucleotide sequence