Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_07515 and PS417_07520 are separated by 191 nucleotides PS417_07520 and PS417_07525 are separated by 134 nucleotides PS417_07525 and PS417_07530 are separated by 13 nucleotides
PS417_07515: PS417_07515 - aconitate hydratase, at 1,651,324 to 1,654,065
_07515
PS417_07520: PS417_07520 - 23S rRNA methyltransferase, at 1,654,257 to 1,655,333
_07520
PS417_07525: PS417_07525 - sulfurtransferase, at 1,655,468 to 1,655,719
_07525
PS417_07530: PS417_07530 - multidrug transporter, at 1,655,733 to 1,657,127
_07530
Position (kb)
1654
1655
1656 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1653.461 kb on + strand, within PS417_07515 at 1653.506 kb on + strand, within PS417_07515 at 1653.596 kb on + strand, within PS417_07515 at 1653.597 kb on - strand, within PS417_07515 at 1653.597 kb on - strand, within PS417_07515 at 1653.597 kb on - strand, within PS417_07515 at 1653.785 kb on - strand, within PS417_07515 at 1654.001 kb on + strand at 1654.001 kb on + strand at 1654.001 kb on + strand at 1654.002 kb on - strand at 1654.002 kb on - strand at 1654.007 kb on + strand at 1654.007 kb on + strand at 1654.014 kb on + strand at 1654.015 kb on - strand at 1654.036 kb on - strand at 1654.036 kb on - strand at 1654.036 kb on - strand at 1654.119 kb on + strand at 1654.120 kb on - strand at 1654.128 kb on - strand at 1654.128 kb on - strand at 1654.128 kb on - strand at 1654.151 kb on - strand at 1654.164 kb on + strand at 1654.164 kb on + strand at 1654.165 kb on - strand at 1654.170 kb on + strand at 1654.170 kb on + strand at 1654.171 kb on - strand at 1654.271 kb on + strand at 1654.271 kb on + strand at 1655.028 kb on - strand, within PS417_07520 at 1655.262 kb on + strand at 1655.390 kb on + strand at 1655.390 kb on + strand at 1655.390 kb on + strand at 1655.390 kb on + strand at 1655.391 kb on - strand at 1655.404 kb on + strand at 1655.467 kb on + strand at 1655.467 kb on + strand at 1656.070 kb on + strand, within PS417_07530 at 1656.071 kb on - strand, within PS417_07530 at 1656.127 kb on + strand, within PS417_07530 at 1656.127 kb on + strand, within PS417_07530 at 1656.128 kb on - strand, within PS417_07530 at 1656.235 kb on + strand, within PS417_07530
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 1,653,461 + PS417_07515 0.78 +0.8 1,653,506 + PS417_07515 0.80 -0.2 1,653,596 + PS417_07515 0.83 -0.7 1,653,597 - PS417_07515 0.83 +0.7 1,653,597 - PS417_07515 0.83 +1.2 1,653,597 - PS417_07515 0.83 -0.1 1,653,785 - PS417_07515 0.90 -0.6 1,654,001 + +0.5 1,654,001 + +2.0 1,654,001 + -1.6 1,654,002 - -2.4 1,654,002 - -0.2 1,654,007 + -0.7 1,654,007 + +0.8 1,654,014 + +1.2 1,654,015 - +0.3 1,654,036 - -1.2 1,654,036 - +1.8 1,654,036 - -1.2 1,654,119 + +1.2 1,654,120 - -1.6 1,654,128 - +1.0 1,654,128 - -0.5 1,654,128 - +1.6 1,654,151 - -1.1 1,654,164 + -0.7 1,654,164 + -0.3 1,654,165 - +1.6 1,654,170 + +1.8 1,654,170 + -0.0 1,654,171 - +0.8 1,654,271 + +1.6 1,654,271 + +0.4 1,655,028 - PS417_07520 0.72 -3.1 1,655,262 + -1.5 1,655,390 + +1.6 1,655,390 + -0.6 1,655,390 + -0.9 1,655,390 + -0.9 1,655,391 - +1.0 1,655,404 + +2.3 1,655,467 + -1.6 1,655,467 + +0.4 1,656,070 + PS417_07530 0.24 -2.5 1,656,071 - PS417_07530 0.24 -1.3 1,656,127 + PS417_07530 0.28 +0.3 1,656,127 + PS417_07530 0.28 -0.7 1,656,128 - PS417_07530 0.28 +0.6 1,656,235 + PS417_07530 0.36 +0.4
Or see this region's nucleotide sequence