Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_00725 and PS417_00730 are separated by 81 nucleotides PS417_00730 and PS417_00735 are separated by 147 nucleotides PS417_00735 and PS417_00740 are separated by 300 nucleotides
PS417_00725: PS417_00725 - RND transporter, at 150,688 to 152,130
_00725
PS417_00730: PS417_00730 - NADH:ubiquinone oxidoreductase subunit N, at 152,212 to 152,844
_00730
PS417_00735: PS417_00735 - hypothetical protein, at 152,992 to 154,488
_00735
PS417_00740: PS417_00740 - membrane protein, at 154,789 to 157,269
_00740
Position (kb) 152
153
154
155 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 152.000 kb on - strand at 152.141 kb on - strand at 152.224 kb on + strand at 152.224 kb on + strand at 152.261 kb on + strand at 152.278 kb on + strand, within PS417_00730 at 152.278 kb on + strand, within PS417_00730 at 152.278 kb on + strand, within PS417_00730 at 152.279 kb on - strand, within PS417_00730 at 152.389 kb on + strand, within PS417_00730 at 152.497 kb on + strand, within PS417_00730 at 152.512 kb on + strand, within PS417_00730 at 152.513 kb on - strand, within PS417_00730 at 152.698 kb on + strand, within PS417_00730 at 152.836 kb on + strand at 152.837 kb on - strand at 152.899 kb on + strand at 152.899 kb on + strand at 152.997 kb on + strand at 152.997 kb on + strand at 153.009 kb on - strand at 153.009 kb on - strand at 153.064 kb on + strand at 153.282 kb on + strand, within PS417_00735 at 153.283 kb on - strand, within PS417_00735 at 153.356 kb on - strand, within PS417_00735 at 153.442 kb on + strand, within PS417_00735 at 153.443 kb on - strand, within PS417_00735 at 153.578 kb on - strand, within PS417_00735 at 153.579 kb on + strand, within PS417_00735 at 153.579 kb on + strand, within PS417_00735 at 153.580 kb on - strand, within PS417_00735 at 153.580 kb on - strand, within PS417_00735 at 153.688 kb on + strand, within PS417_00735 at 153.827 kb on - strand, within PS417_00735 at 153.935 kb on - strand, within PS417_00735 at 153.935 kb on - strand, within PS417_00735 at 154.125 kb on - strand, within PS417_00735 at 154.370 kb on + strand at 154.371 kb on - strand at 154.388 kb on - strand at 154.388 kb on - strand at 154.486 kb on + strand at 154.491 kb on - strand at 154.511 kb on + strand at 154.512 kb on - strand at 154.612 kb on + strand at 154.648 kb on - strand at 154.681 kb on + strand at 154.682 kb on - strand at 154.682 kb on - strand at 154.689 kb on - strand at 154.699 kb on + strand at 154.699 kb on + strand at 154.699 kb on + strand at 154.699 kb on + strand at 154.700 kb on - strand at 154.752 kb on - strand at 154.752 kb on - strand at 154.752 kb on - strand at 154.773 kb on + strand at 154.788 kb on + strand at 154.789 kb on - strand at 154.898 kb on - strand at 155.410 kb on + strand, within PS417_00740 at 155.411 kb on - strand, within PS417_00740 at 155.411 kb on - strand, within PS417_00740
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 152,000 - -3.2 152,141 - -0.9 152,224 + -0.7 152,224 + +0.9 152,261 + -0.1 152,278 + PS417_00730 0.10 +0.1 152,278 + PS417_00730 0.10 -2.8 152,278 + PS417_00730 0.10 -0.3 152,279 - PS417_00730 0.11 +4.4 152,389 + PS417_00730 0.28 -0.2 152,497 + PS417_00730 0.45 +0.3 152,512 + PS417_00730 0.47 -1.9 152,513 - PS417_00730 0.48 -0.6 152,698 + PS417_00730 0.77 +1.8 152,836 + +1.4 152,837 - +0.9 152,899 + -1.2 152,899 + -4.4 152,997 + -0.2 152,997 + -2.6 153,009 - -0.3 153,009 - +1.1 153,064 + -0.0 153,282 + PS417_00735 0.19 +1.0 153,283 - PS417_00735 0.19 +0.7 153,356 - PS417_00735 0.24 -1.9 153,442 + PS417_00735 0.30 +0.3 153,443 - PS417_00735 0.30 -0.9 153,578 - PS417_00735 0.39 -0.7 153,579 + PS417_00735 0.39 -1.7 153,579 + PS417_00735 0.39 +1.1 153,580 - PS417_00735 0.39 +1.2 153,580 - PS417_00735 0.39 -0.2 153,688 + PS417_00735 0.46 -0.1 153,827 - PS417_00735 0.56 +0.1 153,935 - PS417_00735 0.63 +0.8 153,935 - PS417_00735 0.63 +2.1 154,125 - PS417_00735 0.76 +0.2 154,370 + -2.8 154,371 - -3.4 154,388 - +0.9 154,388 - -2.4 154,486 + +1.3 154,491 - +0.5 154,511 + -1.5 154,512 - -1.9 154,612 + +1.6 154,648 - -0.4 154,681 + -0.4 154,682 - -0.4 154,682 - +0.2 154,689 - -1.5 154,699 + -0.0 154,699 + +0.5 154,699 + -1.8 154,699 + +0.1 154,700 - +1.6 154,752 - -0.6 154,752 - +1.0 154,752 - -1.2 154,773 + -0.6 154,788 + +0.4 154,789 - -0.2 154,898 - +0.3 155,410 + PS417_00740 0.25 +0.2 155,411 - PS417_00740 0.25 -1.4 155,411 - PS417_00740 0.25 +0.1
Or see this region's nucleotide sequence