Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_00715 and PS417_00720 overlap by 4 nucleotides PS417_00720 and PS417_00725 overlap by 4 nucleotides PS417_00725 and PS417_00730 are separated by 81 nucleotides PS417_00730 and PS417_00735 are separated by 147 nucleotides
PS417_00715: PS417_00715 - required for octanoate transport, together with NodT, MFP, and FUSC proteins (PS417_00725, PS417_00720, PS417_00710) <small>(from data)</small>, at 149,595 to 149,804
_00715
PS417_00720: PS417_00720 - membrane protein, at 149,801 to 150,691
_00720
PS417_00725: PS417_00725 - RND transporter, at 150,688 to 152,130
_00725
PS417_00730: PS417_00730 - NADH:ubiquinone oxidoreductase subunit N, at 152,212 to 152,844
_00730
PS417_00735: PS417_00735 - hypothetical protein, at 152,992 to 154,488
_00735
Position (kb) 150
151
152
153 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 149.709 kb on + strand, within PS417_00715 at 149.891 kb on + strand, within PS417_00720 at 149.891 kb on + strand, within PS417_00720 at 149.891 kb on + strand at 149.891 kb on + strand, within PS417_00720 at 149.891 kb on + strand, within PS417_00720 at 149.892 kb on - strand, within PS417_00720 at 149.892 kb on - strand, within PS417_00720 at 149.892 kb on - strand, within PS417_00720 at 149.892 kb on - strand, within PS417_00720 at 149.894 kb on + strand, within PS417_00720 at 149.894 kb on + strand, within PS417_00720 at 149.894 kb on + strand, within PS417_00720 at 149.894 kb on + strand, within PS417_00720 at 149.894 kb on + strand, within PS417_00720 at 150.670 kb on + strand at 150.757 kb on + strand at 150.772 kb on - strand at 150.830 kb on + strand at 150.830 kb on + strand at 150.831 kb on - strand at 150.831 kb on - strand at 150.868 kb on + strand, within PS417_00725 at 150.868 kb on + strand, within PS417_00725 at 150.868 kb on + strand, within PS417_00725 at 150.869 kb on - strand, within PS417_00725 at 150.961 kb on + strand, within PS417_00725 at 151.375 kb on + strand, within PS417_00725 at 151.376 kb on - strand, within PS417_00725 at 151.376 kb on - strand, within PS417_00725 at 151.376 kb on - strand, within PS417_00725 at 151.865 kb on - strand, within PS417_00725 at 151.865 kb on - strand, within PS417_00725 at 152.000 kb on - strand at 152.141 kb on - strand at 152.224 kb on + strand at 152.224 kb on + strand at 152.261 kb on + strand at 152.278 kb on + strand, within PS417_00730 at 152.278 kb on + strand, within PS417_00730 at 152.278 kb on + strand, within PS417_00730 at 152.279 kb on - strand, within PS417_00730 at 152.389 kb on + strand, within PS417_00730 at 152.497 kb on + strand, within PS417_00730 at 152.512 kb on + strand, within PS417_00730 at 152.513 kb on - strand, within PS417_00730 at 152.698 kb on + strand, within PS417_00730 at 152.836 kb on + strand at 152.837 kb on - strand at 152.899 kb on + strand at 152.899 kb on + strand at 152.997 kb on + strand at 152.997 kb on + strand at 153.009 kb on - strand at 153.009 kb on - strand at 153.064 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 149,709 + PS417_00715 0.54 -0.6 149,891 + PS417_00720 0.10 -0.5 149,891 + PS417_00720 0.10 -2.7 149,891 + +3.8 149,891 + PS417_00720 0.10 -2.3 149,891 + PS417_00720 0.10 -0.8 149,892 - PS417_00720 0.10 -0.8 149,892 - PS417_00720 0.10 -1.4 149,892 - PS417_00720 0.10 -0.0 149,892 - PS417_00720 0.10 -0.9 149,894 + PS417_00720 0.10 -0.8 149,894 + PS417_00720 0.10 -1.0 149,894 + PS417_00720 0.10 -0.2 149,894 + PS417_00720 0.10 +0.5 149,894 + PS417_00720 0.10 -1.8 150,670 + +0.2 150,757 + -1.9 150,772 - +2.8 150,830 + +0.3 150,830 + +0.8 150,831 - +0.0 150,831 - -1.2 150,868 + PS417_00725 0.12 -3.2 150,868 + PS417_00725 0.12 +2.2 150,868 + PS417_00725 0.12 -0.2 150,869 - PS417_00725 0.13 +1.2 150,961 + PS417_00725 0.19 -0.3 151,375 + PS417_00725 0.48 -0.0 151,376 - PS417_00725 0.48 +0.3 151,376 - PS417_00725 0.48 +0.8 151,376 - PS417_00725 0.48 +0.2 151,865 - PS417_00725 0.82 -1.5 151,865 - PS417_00725 0.82 +0.2 152,000 - -3.2 152,141 - -0.9 152,224 + -0.7 152,224 + +0.9 152,261 + -0.1 152,278 + PS417_00730 0.10 +0.1 152,278 + PS417_00730 0.10 -2.8 152,278 + PS417_00730 0.10 -0.3 152,279 - PS417_00730 0.11 +4.4 152,389 + PS417_00730 0.28 -0.2 152,497 + PS417_00730 0.45 +0.3 152,512 + PS417_00730 0.47 -1.9 152,513 - PS417_00730 0.48 -0.6 152,698 + PS417_00730 0.77 +1.8 152,836 + +1.4 152,837 - +0.9 152,899 + -1.2 152,899 + -4.4 152,997 + -0.2 152,997 + -2.6 153,009 - -0.3 153,009 - +1.1 153,064 + -0.0
Or see this region's nucleotide sequence