Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_07115 and PS417_07120 are separated by 462 nucleotides PS417_07120 and PS417_07125 are separated by 32 nucleotides PS417_07125 and PS417_07130 are separated by 15 nucleotides
PS417_07115: PS417_07115 - L-aspartate oxidase, at 1,572,928 to 1,574,544
_07115
PS417_07120: PS417_07120 - RNA polymerase sigma-H factor, at 1,575,007 to 1,575,588
_07120
PS417_07125: PS417_07125 - RNA polymerase sigma-H factor AlgU, at 1,575,621 to 1,576,208
_07125
PS417_07130: PS417_07130 - RNA polymerase sigma-H factor AlgU, at 1,576,224 to 1,577,186
_07130
Position (kb)
1575
1576 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 1574.320 kb on + strand, within PS417_07115 at 1574.626 kb on - strand at 1574.628 kb on + strand at 1574.628 kb on + strand at 1574.628 kb on + strand at 1574.628 kb on + strand at 1574.628 kb on + strand at 1574.628 kb on + strand at 1574.629 kb on - strand at 1574.629 kb on - strand at 1574.629 kb on - strand at 1574.631 kb on + strand at 1574.632 kb on - strand at 1574.632 kb on - strand at 1574.633 kb on + strand at 1574.633 kb on + strand at 1574.633 kb on + strand at 1574.633 kb on + strand at 1574.633 kb on + strand at 1574.634 kb on - strand at 1574.634 kb on - strand at 1574.634 kb on - strand at 1574.635 kb on + strand at 1574.635 kb on + strand at 1574.635 kb on + strand at 1574.635 kb on + strand at 1574.684 kb on + strand at 1574.684 kb on + strand at 1574.755 kb on - strand at 1574.863 kb on + strand at 1574.945 kb on + strand at 1574.991 kb on - strand at 1575.181 kb on + strand, within PS417_07120 at 1575.230 kb on + strand, within PS417_07120 at 1575.263 kb on - strand, within PS417_07120 at 1575.263 kb on - strand, within PS417_07120 at 1575.302 kb on + strand, within PS417_07120 at 1575.303 kb on - strand, within PS417_07120 at 1575.303 kb on - strand, within PS417_07120 at 1575.475 kb on + strand, within PS417_07120 at 1575.475 kb on + strand, within PS417_07120 at 1576.224 kb on - strand at 1576.349 kb on + strand, within PS417_07130 at 1576.350 kb on - strand, within PS417_07130 at 1576.360 kb on - strand, within PS417_07130 at 1576.575 kb on + strand, within PS417_07130
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 1,574,320 + PS417_07115 0.86 -0.5 1,574,626 - -1.2 1,574,628 + -3.3 1,574,628 + -0.1 1,574,628 + -0.8 1,574,628 + +3.0 1,574,628 + -0.9 1,574,628 + +0.5 1,574,629 - -0.1 1,574,629 - -1.8 1,574,629 - -2.8 1,574,631 + -0.7 1,574,632 - -4.5 1,574,632 - -1.5 1,574,633 + -1.8 1,574,633 + +0.5 1,574,633 + +1.4 1,574,633 + -2.7 1,574,633 + +2.6 1,574,634 - -1.8 1,574,634 - -2.9 1,574,634 - -0.9 1,574,635 + +0.8 1,574,635 + +0.2 1,574,635 + -0.2 1,574,635 + -0.8 1,574,684 + +0.9 1,574,684 + +0.3 1,574,755 - -2.4 1,574,863 + -0.1 1,574,945 + +0.8 1,574,991 - +4.1 1,575,181 + PS417_07120 0.30 -1.1 1,575,230 + PS417_07120 0.38 -2.8 1,575,263 - PS417_07120 0.44 -4.1 1,575,263 - PS417_07120 0.44 -3.3 1,575,302 + PS417_07120 0.51 -3.0 1,575,303 - PS417_07120 0.51 -1.7 1,575,303 - PS417_07120 0.51 +0.8 1,575,475 + PS417_07120 0.80 -2.8 1,575,475 + PS417_07120 0.80 -4.7 1,576,224 - -0.8 1,576,349 + PS417_07130 0.13 +0.3 1,576,350 - PS417_07130 0.13 +2.3 1,576,360 - PS417_07130 0.14 -1.1 1,576,575 + PS417_07130 0.36 -2.9
Or see this region's nucleotide sequence