Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_06745 and PS417_06750 are separated by 116 nucleotides PS417_06750 and PS417_06755 are separated by 90 nucleotides
PS417_06745: PS417_06745 - polysaccharide deacetylase, at 1,492,821 to 1,493,720
_06745
PS417_06750: PS417_06750 - hemolysin, at 1,493,837 to 1,495,339
_06750
PS417_06755: PS417_06755 - porin, at 1,495,430 to 1,496,680
_06755
Position (kb)
1493
1494
1495
1496 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1492.937 kb on - strand, within PS417_06745 at 1492.999 kb on + strand, within PS417_06745 at 1493.782 kb on + strand at 1493.847 kb on + strand at 1493.847 kb on + strand at 1493.847 kb on + strand at 1493.848 kb on - strand at 1493.848 kb on - strand at 1493.848 kb on - strand at 1493.943 kb on - strand at 1494.036 kb on + strand, within PS417_06750 at 1494.036 kb on + strand, within PS417_06750 at 1494.036 kb on + strand, within PS417_06750 at 1494.037 kb on - strand, within PS417_06750 at 1494.078 kb on + strand, within PS417_06750 at 1494.079 kb on - strand, within PS417_06750 at 1494.117 kb on + strand, within PS417_06750 at 1494.226 kb on + strand, within PS417_06750 at 1494.227 kb on - strand, within PS417_06750 at 1494.244 kb on - strand, within PS417_06750 at 1494.280 kb on + strand, within PS417_06750 at 1494.280 kb on + strand, within PS417_06750 at 1494.281 kb on - strand, within PS417_06750 at 1494.422 kb on + strand, within PS417_06750 at 1494.502 kb on - strand, within PS417_06750 at 1494.556 kb on - strand, within PS417_06750 at 1494.633 kb on + strand, within PS417_06750 at 1494.634 kb on - strand, within PS417_06750 at 1494.639 kb on - strand, within PS417_06750 at 1494.726 kb on + strand, within PS417_06750 at 1494.727 kb on - strand, within PS417_06750 at 1494.727 kb on - strand, within PS417_06750 at 1494.983 kb on - strand, within PS417_06750 at 1495.002 kb on + strand, within PS417_06750 at 1495.003 kb on - strand, within PS417_06750 at 1495.003 kb on - strand, within PS417_06750 at 1495.004 kb on + strand, within PS417_06750 at 1495.005 kb on - strand, within PS417_06750 at 1495.041 kb on + strand, within PS417_06750 at 1495.229 kb on + strand at 1495.229 kb on + strand at 1495.230 kb on - strand at 1495.262 kb on - strand at 1495.343 kb on + strand at 1495.343 kb on + strand at 1495.344 kb on - strand at 1495.353 kb on - strand at 1495.421 kb on - strand at 1495.421 kb on - strand at 1495.491 kb on + strand at 1495.560 kb on + strand at 1495.636 kb on - strand, within PS417_06755 at 1495.694 kb on + strand, within PS417_06755 at 1495.694 kb on + strand, within PS417_06755 at 1495.737 kb on + strand, within PS417_06755 at 1495.738 kb on - strand, within PS417_06755 at 1495.822 kb on - strand, within PS417_06755 at 1495.822 kb on - strand, within PS417_06755 at 1495.839 kb on + strand, within PS417_06755 at 1495.992 kb on + strand, within PS417_06755 at 1495.992 kb on + strand, within PS417_06755 at 1495.992 kb on + strand, within PS417_06755 at 1495.993 kb on - strand, within PS417_06755
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 1,492,937 - PS417_06745 0.13 -0.8 1,492,999 + PS417_06745 0.20 -1.1 1,493,782 + -1.1 1,493,847 + -0.9 1,493,847 + -1.9 1,493,847 + -0.1 1,493,848 - +1.2 1,493,848 - +1.1 1,493,848 - -1.5 1,493,943 - -1.0 1,494,036 + PS417_06750 0.13 -1.4 1,494,036 + PS417_06750 0.13 -1.1 1,494,036 + PS417_06750 0.13 +0.5 1,494,037 - PS417_06750 0.13 -0.5 1,494,078 + PS417_06750 0.16 +0.9 1,494,079 - PS417_06750 0.16 -0.1 1,494,117 + PS417_06750 0.19 +0.9 1,494,226 + PS417_06750 0.26 +1.8 1,494,227 - PS417_06750 0.26 +1.3 1,494,244 - PS417_06750 0.27 +0.8 1,494,280 + PS417_06750 0.29 +2.0 1,494,280 + PS417_06750 0.29 +0.4 1,494,281 - PS417_06750 0.30 -0.8 1,494,422 + PS417_06750 0.39 +0.4 1,494,502 - PS417_06750 0.44 -0.4 1,494,556 - PS417_06750 0.48 -2.7 1,494,633 + PS417_06750 0.53 -0.8 1,494,634 - PS417_06750 0.53 -0.8 1,494,639 - PS417_06750 0.53 +0.9 1,494,726 + PS417_06750 0.59 +0.4 1,494,727 - PS417_06750 0.59 +1.9 1,494,727 - PS417_06750 0.59 +0.5 1,494,983 - PS417_06750 0.76 +0.9 1,495,002 + PS417_06750 0.78 +2.0 1,495,003 - PS417_06750 0.78 +0.7 1,495,003 - PS417_06750 0.78 -0.2 1,495,004 + PS417_06750 0.78 +0.2 1,495,005 - PS417_06750 0.78 +0.2 1,495,041 + PS417_06750 0.80 -1.8 1,495,229 + -1.8 1,495,229 + -0.3 1,495,230 - +1.0 1,495,262 - -2.2 1,495,343 + -1.3 1,495,343 + +0.3 1,495,344 - -2.1 1,495,353 - -0.3 1,495,421 - -1.0 1,495,421 - +0.4 1,495,491 + +0.5 1,495,560 + -1.0 1,495,636 - PS417_06755 0.16 -2.8 1,495,694 + PS417_06755 0.21 +0.1 1,495,694 + PS417_06755 0.21 -2.7 1,495,737 + PS417_06755 0.25 +0.8 1,495,738 - PS417_06755 0.25 -1.5 1,495,822 - PS417_06755 0.31 -0.1 1,495,822 - PS417_06755 0.31 -0.5 1,495,839 + PS417_06755 0.33 +0.0 1,495,992 + PS417_06755 0.45 -1.3 1,495,992 + PS417_06755 0.45 +1.4 1,495,992 + PS417_06755 0.45 +0.7 1,495,993 - PS417_06755 0.45 -0.3
Or see this region's nucleotide sequence