Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_06710 and PS417_06715 are separated by 17 nucleotides PS417_06715 and PS417_06720 are separated by 1 nucleotides PS417_06720 and PS417_06725 are separated by 137 nucleotides
PS417_06710: PS417_06710 - beta-ketoadipyl CoA thiolase, at 1,486,185 to 1,487,387
_06710
PS417_06715: PS417_06715 - protocatechuate 3,4-dioxygenase, at 1,487,405 to 1,488,109
_06715
PS417_06720: PS417_06720 - protocatechuate 3,4-dioxygenase, at 1,488,111 to 1,488,677
_06720
PS417_06725: PS417_06725 - alpha-ketoglutarate permease, at 1,488,815 to 1,490,110
_06725
Position (kb)
1488
1489 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1487.385 kb on + strand at 1487.386 kb on - strand at 1487.386 kb on - strand at 1487.386 kb on - strand at 1487.389 kb on - strand at 1487.457 kb on - strand at 1487.457 kb on - strand at 1487.471 kb on + strand at 1487.930 kb on + strand, within PS417_06715 at 1487.930 kb on + strand, within PS417_06715 at 1487.930 kb on + strand at 1487.930 kb on + strand, within PS417_06715 at 1487.930 kb on + strand, within PS417_06715 at 1487.930 kb on + strand, within PS417_06715 at 1487.930 kb on + strand, within PS417_06715 at 1487.930 kb on + strand, within PS417_06715 at 1487.930 kb on + strand, within PS417_06715 at 1487.931 kb on - strand, within PS417_06715 at 1487.931 kb on - strand, within PS417_06715 at 1487.931 kb on - strand, within PS417_06715 at 1487.931 kb on - strand, within PS417_06715 at 1487.931 kb on - strand, within PS417_06715 at 1487.970 kb on - strand, within PS417_06715 at 1487.970 kb on - strand, within PS417_06715 at 1488.045 kb on - strand at 1488.150 kb on + strand at 1488.154 kb on - strand at 1488.309 kb on + strand, within PS417_06720 at 1488.309 kb on + strand, within PS417_06720 at 1488.309 kb on + strand, within PS417_06720 at 1488.309 kb on + strand, within PS417_06720 at 1488.310 kb on - strand, within PS417_06720 at 1488.310 kb on - strand, within PS417_06720 at 1488.310 kb on - strand, within PS417_06720 at 1488.310 kb on - strand, within PS417_06720 at 1488.310 kb on - strand, within PS417_06720 at 1488.405 kb on - strand, within PS417_06720 at 1488.463 kb on + strand, within PS417_06720 at 1488.464 kb on - strand, within PS417_06720 at 1488.467 kb on - strand, within PS417_06720 at 1488.467 kb on - strand, within PS417_06720 at 1488.585 kb on - strand, within PS417_06720 at 1488.619 kb on - strand, within PS417_06720 at 1488.619 kb on - strand, within PS417_06720 at 1488.738 kb on - strand at 1488.777 kb on - strand at 1488.777 kb on - strand at 1488.777 kb on - strand at 1488.787 kb on + strand at 1488.917 kb on + strand at 1488.918 kb on - strand at 1488.918 kb on - strand at 1488.918 kb on - strand at 1488.923 kb on + strand at 1488.924 kb on - strand at 1488.941 kb on + strand at 1488.942 kb on - strand at 1489.148 kb on + strand, within PS417_06725 at 1489.148 kb on + strand, within PS417_06725 at 1489.245 kb on - strand, within PS417_06725 at 1489.245 kb on - strand, within PS417_06725 at 1489.245 kb on - strand, within PS417_06725 at 1489.298 kb on + strand, within PS417_06725 at 1489.302 kb on + strand, within PS417_06725 at 1489.303 kb on - strand, within PS417_06725 at 1489.598 kb on + strand, within PS417_06725 at 1489.598 kb on + strand, within PS417_06725 at 1489.598 kb on + strand, within PS417_06725 at 1489.599 kb on - strand, within PS417_06725 at 1489.599 kb on - strand, within PS417_06725 at 1489.652 kb on + strand, within PS417_06725 at 1489.652 kb on + strand, within PS417_06725 at 1489.652 kb on + strand, within PS417_06725 at 1489.653 kb on - strand, within PS417_06725 at 1489.653 kb on - strand, within PS417_06725 at 1489.653 kb on - strand, within PS417_06725
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 1,487,385 + -1.8 1,487,386 - -2.2 1,487,386 - -2.5 1,487,386 - -0.7 1,487,389 - +0.8 1,487,457 - +0.6 1,487,457 - +0.7 1,487,471 + -3.2 1,487,930 + PS417_06715 0.74 -0.3 1,487,930 + PS417_06715 0.74 +0.5 1,487,930 + +1.8 1,487,930 + PS417_06715 0.74 -1.3 1,487,930 + PS417_06715 0.74 +1.4 1,487,930 + PS417_06715 0.74 -3.8 1,487,930 + PS417_06715 0.74 -2.6 1,487,930 + PS417_06715 0.74 -1.9 1,487,930 + PS417_06715 0.74 -1.7 1,487,931 - PS417_06715 0.75 -2.2 1,487,931 - PS417_06715 0.75 -2.1 1,487,931 - PS417_06715 0.75 +0.4 1,487,931 - PS417_06715 0.75 +0.8 1,487,931 - PS417_06715 0.75 +0.3 1,487,970 - PS417_06715 0.80 +1.5 1,487,970 - PS417_06715 0.80 +1.8 1,488,045 - +0.1 1,488,150 + +1.8 1,488,154 - -0.1 1,488,309 + PS417_06720 0.35 -2.0 1,488,309 + PS417_06720 0.35 +1.1 1,488,309 + PS417_06720 0.35 +0.4 1,488,309 + PS417_06720 0.35 -0.9 1,488,310 - PS417_06720 0.35 +2.0 1,488,310 - PS417_06720 0.35 +0.4 1,488,310 - PS417_06720 0.35 -0.1 1,488,310 - PS417_06720 0.35 +0.0 1,488,310 - PS417_06720 0.35 +0.2 1,488,405 - PS417_06720 0.52 -1.0 1,488,463 + PS417_06720 0.62 -0.1 1,488,464 - PS417_06720 0.62 -0.2 1,488,467 - PS417_06720 0.63 -2.5 1,488,467 - PS417_06720 0.63 -2.0 1,488,585 - PS417_06720 0.84 -3.2 1,488,619 - PS417_06720 0.90 +0.3 1,488,619 - PS417_06720 0.90 +0.3 1,488,738 - +0.0 1,488,777 - +0.7 1,488,777 - +2.2 1,488,777 - -2.3 1,488,787 + -0.2 1,488,917 + -1.1 1,488,918 - -0.2 1,488,918 - +0.6 1,488,918 - -0.2 1,488,923 + -0.1 1,488,924 - +1.6 1,488,941 + +0.8 1,488,942 - -0.7 1,489,148 + PS417_06725 0.26 +0.3 1,489,148 + PS417_06725 0.26 -3.7 1,489,245 - PS417_06725 0.33 -0.2 1,489,245 - PS417_06725 0.33 -0.1 1,489,245 - PS417_06725 0.33 -0.2 1,489,298 + PS417_06725 0.37 -0.2 1,489,302 + PS417_06725 0.38 -1.7 1,489,303 - PS417_06725 0.38 -0.7 1,489,598 + PS417_06725 0.60 +0.5 1,489,598 + PS417_06725 0.60 +0.5 1,489,598 + PS417_06725 0.60 -0.4 1,489,599 - PS417_06725 0.60 -0.4 1,489,599 - PS417_06725 0.60 -2.8 1,489,652 + PS417_06725 0.65 -1.9 1,489,652 + PS417_06725 0.65 -1.2 1,489,652 + PS417_06725 0.65 -0.3 1,489,653 - PS417_06725 0.65 -0.4 1,489,653 - PS417_06725 0.65 -1.7 1,489,653 - PS417_06725 0.65 -2.2
Or see this region's nucleotide sequence