Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_00640 and PS417_00645 are separated by 99 nucleotides PS417_00645 and PS417_00650 are separated by 52 nucleotides PS417_00650 and PS417_00655 are separated by 102 nucleotides
PS417_00640: PS417_00640 - ABC transporter permease, at 131,045 to 131,893
_00640
PS417_00645: PS417_00645 - hypothetical protein, at 131,993 to 132,619
_00645
PS417_00650: PS417_00650 - LysR family transcriptional regulator, at 132,672 to 133,592
_00650
PS417_00655: PS417_00655 - DSBA oxidoreductase, at 133,695 to 135,230
_00655
Position (kb) 132
133
134 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 131.747 kb on + strand, within PS417_00640 at 131.747 kb on + strand, within PS417_00640 at 131.748 kb on - strand, within PS417_00640 at 131.748 kb on - strand, within PS417_00640 at 131.748 kb on - strand, within PS417_00640 at 131.774 kb on + strand, within PS417_00640 at 131.774 kb on + strand, within PS417_00640 at 131.775 kb on - strand, within PS417_00640 at 131.775 kb on - strand, within PS417_00640 at 131.775 kb on - strand, within PS417_00640 at 131.987 kb on - strand at 132.024 kb on + strand at 132.024 kb on + strand at 132.136 kb on + strand, within PS417_00645 at 132.136 kb on + strand, within PS417_00645 at 132.152 kb on - strand, within PS417_00645 at 132.290 kb on - strand, within PS417_00645 at 132.353 kb on + strand, within PS417_00645 at 132.353 kb on + strand, within PS417_00645 at 132.353 kb on + strand, within PS417_00645 at 132.354 kb on - strand, within PS417_00645 at 132.482 kb on - strand, within PS417_00645 at 132.550 kb on + strand, within PS417_00645 at 132.550 kb on + strand, within PS417_00645 at 132.579 kb on - strand at 132.588 kb on - strand at 132.618 kb on - strand at 133.242 kb on + strand, within PS417_00650 at 133.612 kb on + strand at 133.613 kb on - strand at 133.669 kb on + strand at 133.760 kb on - strand at 133.760 kb on - strand at 133.872 kb on + strand, within PS417_00655 at 133.872 kb on + strand, within PS417_00655 at 133.873 kb on - strand, within PS417_00655 at 133.876 kb on + strand, within PS417_00655 at 133.877 kb on - strand, within PS417_00655 at 133.877 kb on - strand, within PS417_00655 at 133.877 kb on - strand, within PS417_00655 at 133.877 kb on - strand, within PS417_00655 at 134.044 kb on - strand, within PS417_00655 at 134.044 kb on - strand, within PS417_00655 at 134.152 kb on - strand, within PS417_00655 at 134.218 kb on + strand, within PS417_00655 at 134.229 kb on + strand, within PS417_00655 at 134.230 kb on - strand, within PS417_00655
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 131,747 + PS417_00640 0.83 +0.6 131,747 + PS417_00640 0.83 +1.5 131,748 - PS417_00640 0.83 +0.2 131,748 - PS417_00640 0.83 -0.5 131,748 - PS417_00640 0.83 -0.4 131,774 + PS417_00640 0.86 -1.3 131,774 + PS417_00640 0.86 +0.4 131,775 - PS417_00640 0.86 -1.3 131,775 - PS417_00640 0.86 +0.1 131,775 - PS417_00640 0.86 -1.6 131,987 - +2.0 132,024 + +0.8 132,024 + +0.0 132,136 + PS417_00645 0.23 -0.0 132,136 + PS417_00645 0.23 -0.0 132,152 - PS417_00645 0.25 +1.0 132,290 - PS417_00645 0.47 +1.1 132,353 + PS417_00645 0.57 +1.2 132,353 + PS417_00645 0.57 -0.9 132,353 + PS417_00645 0.57 +0.5 132,354 - PS417_00645 0.58 -1.6 132,482 - PS417_00645 0.78 +0.7 132,550 + PS417_00645 0.89 -0.7 132,550 + PS417_00645 0.89 +0.1 132,579 - -1.2 132,588 - -1.8 132,618 - -0.5 133,242 + PS417_00650 0.62 -3.4 133,612 + +2.5 133,613 - -1.9 133,669 + -0.3 133,760 - -0.3 133,760 - +0.0 133,872 + PS417_00655 0.12 -0.8 133,872 + PS417_00655 0.12 +0.8 133,873 - PS417_00655 0.12 +0.5 133,876 + PS417_00655 0.12 -2.8 133,877 - PS417_00655 0.12 +0.2 133,877 - PS417_00655 0.12 +0.9 133,877 - PS417_00655 0.12 +1.3 133,877 - PS417_00655 0.12 -0.5 134,044 - PS417_00655 0.23 -1.4 134,044 - PS417_00655 0.23 +0.0 134,152 - PS417_00655 0.30 +0.5 134,218 + PS417_00655 0.34 -2.2 134,229 + PS417_00655 0.35 -1.2 134,230 - PS417_00655 0.35 -2.0
Or see this region's nucleotide sequence