Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_06530 and PS417_06535 are separated by 27 nucleotides PS417_06535 and PS417_06540 are separated by 269 nucleotides PS417_06540 and PS417_06545 are separated by 725 nucleotides
PS417_06530: PS417_06530 - membrane protein, at 1,447,205 to 1,448,347
_06530
PS417_06535: PS417_06535 - pyridoxine 5'-phosphate oxidase, at 1,448,375 to 1,449,022
_06535
PS417_06540: PS417_06540 - membrane protein, at 1,449,292 to 1,449,756
_06540
PS417_06545: PS417_06545 - pH-dependent sodium/proton antiporter, at 1,450,482 to 1,451,675
_06545
Position (kb)
1449
1450 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1448.366 kb on + strand at 1449.096 kb on + strand at 1449.096 kb on + strand at 1449.132 kb on - strand at 1449.143 kb on - strand at 1449.143 kb on - strand at 1449.155 kb on + strand at 1449.155 kb on + strand at 1449.156 kb on - strand at 1449.156 kb on - strand at 1449.197 kb on - strand at 1449.197 kb on - strand at 1449.199 kb on + strand at 1449.199 kb on + strand at 1449.261 kb on + strand at 1449.291 kb on + strand at 1449.292 kb on - strand at 1449.292 kb on - strand at 1449.298 kb on - strand at 1449.298 kb on - strand at 1449.298 kb on - strand at 1449.298 kb on - strand at 1449.309 kb on - strand at 1449.309 kb on - strand at 1449.459 kb on + strand, within PS417_06540 at 1449.459 kb on + strand, within PS417_06540 at 1449.623 kb on + strand, within PS417_06540 at 1449.735 kb on + strand at 1449.735 kb on + strand at 1449.736 kb on - strand at 1449.736 kb on - strand at 1449.736 kb on - strand at 1449.736 kb on - strand at 1449.738 kb on + strand at 1449.739 kb on - strand at 1449.773 kb on + strand at 1449.814 kb on + strand at 1449.815 kb on - strand at 1449.868 kb on + strand at 1449.869 kb on - strand at 1449.947 kb on - strand at 1449.950 kb on - strand at 1449.950 kb on - strand at 1450.045 kb on + strand at 1450.073 kb on - strand at 1450.128 kb on - strand at 1450.140 kb on - strand at 1450.193 kb on + strand at 1450.194 kb on - strand at 1450.194 kb on - strand at 1450.296 kb on + strand at 1450.297 kb on - strand at 1450.297 kb on - strand at 1450.322 kb on + strand at 1450.322 kb on + strand at 1450.343 kb on + strand at 1450.344 kb on - strand at 1450.357 kb on + strand at 1450.377 kb on + strand at 1450.390 kb on + strand at 1450.391 kb on - strand at 1450.450 kb on - strand at 1450.450 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 1,448,366 + -0.8 1,449,096 + +3.3 1,449,096 + -0.1 1,449,132 - +1.2 1,449,143 - -1.9 1,449,143 - +0.4 1,449,155 + +1.9 1,449,155 + -0.5 1,449,156 - +0.6 1,449,156 - +0.6 1,449,197 - +0.2 1,449,197 - -0.6 1,449,199 + -1.0 1,449,199 + +2.8 1,449,261 + -0.2 1,449,291 + +0.2 1,449,292 - -2.2 1,449,292 - +0.5 1,449,298 - +0.7 1,449,298 - +0.7 1,449,298 - -1.0 1,449,298 - -0.2 1,449,309 - +1.1 1,449,309 - +1.1 1,449,459 + PS417_06540 0.36 -3.4 1,449,459 + PS417_06540 0.36 -0.8 1,449,623 + PS417_06540 0.71 -0.8 1,449,735 + -2.1 1,449,735 + -0.7 1,449,736 - -2.2 1,449,736 - -0.9 1,449,736 - -0.2 1,449,736 - -2.7 1,449,738 + -0.8 1,449,739 - -0.4 1,449,773 + +3.6 1,449,814 + -0.1 1,449,815 - -1.2 1,449,868 + -1.7 1,449,869 - +1.1 1,449,947 - +1.0 1,449,950 - -3.0 1,449,950 - +0.3 1,450,045 + -3.5 1,450,073 - -0.5 1,450,128 - -1.6 1,450,140 - +1.5 1,450,193 + -0.2 1,450,194 - +2.3 1,450,194 - -0.3 1,450,296 + +0.4 1,450,297 - +0.2 1,450,297 - +0.6 1,450,322 + -2.6 1,450,322 + +1.4 1,450,343 + -0.9 1,450,344 - -0.4 1,450,357 + +0.7 1,450,377 + -0.2 1,450,390 + -1.0 1,450,391 - +0.9 1,450,450 - +0.9 1,450,450 - +0.6
Or see this region's nucleotide sequence