Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_05740 and PS417_05745 are separated by 1 nucleotides PS417_05745 and PS417_05755 are separated by 264 nucleotides PS417_05755 and PS417_05760 overlap by 1 nucleotides PS417_05760 and PS417_05765 are separated by 52 nucleotides
PS417_05740: PS417_05740 - hypothetical protein, at 1,286,069 to 1,286,851
_05740
PS417_05745: PS417_05745 - hypothetical protein, at 1,286,853 to 1,287,155
_05745
PS417_05755: PS417_05755 - tail protein, at 1,287,420 to 1,288,586
_05755
PS417_05760: PS417_05760 - phage tail protein, at 1,288,586 to 1,289,092
_05760
PS417_05765: PS417_05765 - hypothetical protein, at 1,289,145 to 1,289,717
_05765
Position (kb)
1287
1288
1289 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1286.521 kb on + strand, within PS417_05740 at 1286.532 kb on - strand at 1286.532 kb on - strand, within PS417_05740 at 1286.532 kb on - strand, within PS417_05740 at 1286.532 kb on - strand, within PS417_05740 at 1286.618 kb on + strand, within PS417_05740 at 1286.619 kb on - strand, within PS417_05740 at 1286.619 kb on - strand, within PS417_05740 at 1286.635 kb on + strand at 1286.636 kb on - strand, within PS417_05740 at 1286.639 kb on + strand, within PS417_05740 at 1286.639 kb on + strand, within PS417_05740 at 1286.640 kb on - strand, within PS417_05740 at 1286.640 kb on - strand, within PS417_05740 at 1286.640 kb on - strand, within PS417_05740 at 1286.640 kb on - strand, within PS417_05740 at 1286.641 kb on + strand at 1286.641 kb on + strand, within PS417_05740 at 1286.808 kb on - strand at 1286.852 kb on + strand at 1286.875 kb on + strand at 1286.960 kb on - strand, within PS417_05745 at 1286.988 kb on - strand, within PS417_05745 at 1287.008 kb on + strand, within PS417_05745 at 1287.009 kb on - strand, within PS417_05745 at 1287.030 kb on + strand, within PS417_05745 at 1287.030 kb on + strand, within PS417_05745 at 1287.031 kb on - strand at 1287.031 kb on - strand, within PS417_05745 at 1287.032 kb on + strand, within PS417_05745 at 1287.032 kb on + strand, within PS417_05745 at 1287.033 kb on - strand, within PS417_05745 at 1287.069 kb on + strand, within PS417_05745 at 1287.070 kb on - strand, within PS417_05745 at 1287.149 kb on + strand at 1287.150 kb on - strand at 1287.434 kb on + strand at 1287.435 kb on - strand at 1287.435 kb on - strand at 1287.646 kb on - strand, within PS417_05755 at 1287.678 kb on + strand, within PS417_05755 at 1287.679 kb on - strand, within PS417_05755 at 1287.836 kb on + strand, within PS417_05755 at 1287.962 kb on - strand, within PS417_05755 at 1287.962 kb on - strand, within PS417_05755 at 1287.962 kb on - strand, within PS417_05755 at 1288.006 kb on - strand, within PS417_05755 at 1288.140 kb on + strand, within PS417_05755 at 1288.141 kb on - strand, within PS417_05755 at 1288.197 kb on + strand, within PS417_05755 at 1288.197 kb on + strand, within PS417_05755 at 1288.198 kb on - strand, within PS417_05755 at 1288.270 kb on - strand, within PS417_05755 at 1288.432 kb on - strand at 1288.432 kb on - strand, within PS417_05755 at 1288.464 kb on + strand, within PS417_05755 at 1288.530 kb on + strand at 1288.531 kb on - strand at 1288.531 kb on - strand at 1288.592 kb on - strand at 1288.592 kb on - strand at 1288.871 kb on + strand, within PS417_05760 at 1288.872 kb on - strand, within PS417_05760 at 1288.872 kb on - strand, within PS417_05760 at 1288.880 kb on + strand, within PS417_05760 at 1288.881 kb on - strand, within PS417_05760 at 1288.976 kb on + strand, within PS417_05760 at 1288.976 kb on + strand, within PS417_05760 at 1288.976 kb on + strand, within PS417_05760 at 1288.976 kb on + strand, within PS417_05760 at 1289.019 kb on + strand, within PS417_05760 at 1289.020 kb on - strand, within PS417_05760 at 1289.020 kb on - strand, within PS417_05760 at 1289.020 kb on - strand, within PS417_05760 at 1289.123 kb on + strand at 1289.124 kb on - strand at 1289.124 kb on - strand at 1289.140 kb on - strand at 1289.235 kb on + strand, within PS417_05765 at 1289.235 kb on + strand, within PS417_05765 at 1289.235 kb on + strand, within PS417_05765 at 1289.238 kb on + strand, within PS417_05765 at 1289.239 kb on - strand, within PS417_05765 at 1289.361 kb on + strand, within PS417_05765 at 1289.362 kb on - strand, within PS417_05765 at 1289.379 kb on + strand, within PS417_05765 at 1289.380 kb on - strand, within PS417_05765 at 1289.380 kb on - strand, within PS417_05765 at 1289.383 kb on + strand, within PS417_05765 at 1289.383 kb on + strand, within PS417_05765 at 1289.383 kb on + strand, within PS417_05765 at 1289.384 kb on - strand, within PS417_05765 at 1289.384 kb on - strand, within PS417_05765
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 1,286,521 + PS417_05740 0.58 -0.1 1,286,532 - -0.8 1,286,532 - PS417_05740 0.59 +0.1 1,286,532 - PS417_05740 0.59 +2.6 1,286,532 - PS417_05740 0.59 +2.0 1,286,618 + PS417_05740 0.70 -1.0 1,286,619 - PS417_05740 0.70 -0.6 1,286,619 - PS417_05740 0.70 -0.4 1,286,635 + +1.8 1,286,636 - PS417_05740 0.72 -4.6 1,286,639 + PS417_05740 0.73 -2.9 1,286,639 + PS417_05740 0.73 -1.8 1,286,640 - PS417_05740 0.73 -2.2 1,286,640 - PS417_05740 0.73 +0.4 1,286,640 - PS417_05740 0.73 -0.9 1,286,640 - PS417_05740 0.73 +1.0 1,286,641 + +1.8 1,286,641 + PS417_05740 0.73 -1.5 1,286,808 - +0.0 1,286,852 + +0.4 1,286,875 + +0.1 1,286,960 - PS417_05745 0.35 -0.6 1,286,988 - PS417_05745 0.45 +0.1 1,287,008 + PS417_05745 0.51 -2.5 1,287,009 - PS417_05745 0.51 -0.5 1,287,030 + PS417_05745 0.58 +0.4 1,287,030 + PS417_05745 0.58 +0.4 1,287,031 - +2.4 1,287,031 - PS417_05745 0.59 -0.8 1,287,032 + PS417_05745 0.59 -0.5 1,287,032 + PS417_05745 0.59 -0.4 1,287,033 - PS417_05745 0.59 -1.5 1,287,069 + PS417_05745 0.71 +0.2 1,287,070 - PS417_05745 0.72 -0.5 1,287,149 + -1.2 1,287,150 - +0.2 1,287,434 + +0.5 1,287,435 - +1.7 1,287,435 - +0.3 1,287,646 - PS417_05755 0.19 -4.0 1,287,678 + PS417_05755 0.22 +0.2 1,287,679 - PS417_05755 0.22 +0.9 1,287,836 + PS417_05755 0.36 +0.3 1,287,962 - PS417_05755 0.46 -0.9 1,287,962 - PS417_05755 0.46 -0.4 1,287,962 - PS417_05755 0.46 -2.2 1,288,006 - PS417_05755 0.50 +0.1 1,288,140 + PS417_05755 0.62 -0.5 1,288,141 - PS417_05755 0.62 -2.7 1,288,197 + PS417_05755 0.67 -1.8 1,288,197 + PS417_05755 0.67 -2.4 1,288,198 - PS417_05755 0.67 -1.6 1,288,270 - PS417_05755 0.73 -0.1 1,288,432 - +0.2 1,288,432 - PS417_05755 0.87 -0.9 1,288,464 + PS417_05755 0.89 +0.6 1,288,530 + +0.8 1,288,531 - +0.4 1,288,531 - +1.6 1,288,592 - -0.2 1,288,592 - -2.8 1,288,871 + PS417_05760 0.56 -0.0 1,288,872 - PS417_05760 0.56 +0.2 1,288,872 - PS417_05760 0.56 -0.5 1,288,880 + PS417_05760 0.58 -2.0 1,288,881 - PS417_05760 0.58 +0.5 1,288,976 + PS417_05760 0.77 -0.7 1,288,976 + PS417_05760 0.77 -1.3 1,288,976 + PS417_05760 0.77 -0.9 1,288,976 + PS417_05760 0.77 +0.7 1,289,019 + PS417_05760 0.85 +2.3 1,289,020 - PS417_05760 0.86 -1.8 1,289,020 - PS417_05760 0.86 +0.2 1,289,020 - PS417_05760 0.86 -1.4 1,289,123 + +1.3 1,289,124 - -1.8 1,289,124 - -0.7 1,289,140 - +0.7 1,289,235 + PS417_05765 0.16 +1.6 1,289,235 + PS417_05765 0.16 +0.2 1,289,235 + PS417_05765 0.16 -0.3 1,289,238 + PS417_05765 0.16 +0.3 1,289,239 - PS417_05765 0.16 -0.0 1,289,361 + PS417_05765 0.38 -2.0 1,289,362 - PS417_05765 0.38 +0.5 1,289,379 + PS417_05765 0.41 +0.9 1,289,380 - PS417_05765 0.41 +1.3 1,289,380 - PS417_05765 0.41 +0.1 1,289,383 + PS417_05765 0.42 -0.1 1,289,383 + PS417_05765 0.42 +0.5 1,289,383 + PS417_05765 0.42 +0.2 1,289,384 - PS417_05765 0.42 -0.2 1,289,384 - PS417_05765 0.42 +0.8
Or see this region's nucleotide sequence