Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_05540 and PS417_05545 are separated by 157 nucleotides PS417_05545 and PS417_05550 overlap by 4 nucleotides PS417_05550 and PS417_05555 overlap by 1 nucleotides
PS417_05540: PS417_05540 - histidine kinase, at 1,246,320 to 1,248,221
_05540
PS417_05545: PS417_05545 - amino acid ABC transporter ATP-binding protein, at 1,248,379 to 1,249,113
_05545
PS417_05550: PS417_05550 - amino acid ABC transporter permease, at 1,249,110 to 1,249,781
_05550
PS417_05555: PS417_05555 - amino acid ABC transporter permease, at 1,249,781 to 1,250,527
_05555
Position (kb) 1248
1249
1250 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 1247.470 kb on + strand, within PS417_05540 at 1247.473 kb on + strand, within PS417_05540 at 1247.473 kb on + strand, within PS417_05540 at 1247.695 kb on + strand, within PS417_05540 at 1247.696 kb on - strand, within PS417_05540 at 1247.696 kb on - strand at 1247.801 kb on - strand, within PS417_05540 at 1247.823 kb on - strand at 1247.860 kb on + strand, within PS417_05540 at 1247.860 kb on + strand, within PS417_05540 at 1247.860 kb on + strand, within PS417_05540 at 1247.860 kb on + strand, within PS417_05540 at 1247.861 kb on - strand, within PS417_05540 at 1247.861 kb on - strand, within PS417_05540 at 1247.861 kb on - strand, within PS417_05540 at 1247.861 kb on - strand, within PS417_05540 at 1247.990 kb on - strand, within PS417_05540 at 1247.990 kb on - strand, within PS417_05540 at 1247.996 kb on - strand, within PS417_05540 at 1248.076 kb on + strand at 1248.080 kb on - strand at 1248.580 kb on + strand, within PS417_05545 at 1248.580 kb on + strand, within PS417_05545 at 1248.581 kb on - strand, within PS417_05545 at 1248.590 kb on + strand, within PS417_05545 at 1248.590 kb on - strand at 1248.590 kb on - strand, within PS417_05545 at 1248.800 kb on - strand, within PS417_05545 at 1248.800 kb on - strand, within PS417_05545 at 1249.083 kb on - strand at 1249.095 kb on + strand at 1249.096 kb on - strand at 1249.096 kb on - strand at 1249.375 kb on + strand, within PS417_05550 at 1249.375 kb on + strand, within PS417_05550 at 1249.376 kb on - strand at 1249.559 kb on - strand, within PS417_05550 at 1249.600 kb on + strand, within PS417_05550 at 1249.610 kb on - strand, within PS417_05550 at 1249.610 kb on - strand, within PS417_05550 at 1249.782 kb on + strand at 1249.783 kb on - strand at 1249.872 kb on + strand, within PS417_05555 at 1249.872 kb on + strand, within PS417_05555 at 1249.873 kb on - strand, within PS417_05555 at 1250.062 kb on - strand, within PS417_05555
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 1,247,470 + PS417_05540 0.60 -3.9 1,247,473 + PS417_05540 0.61 +0.2 1,247,473 + PS417_05540 0.61 -1.8 1,247,695 + PS417_05540 0.72 -2.0 1,247,696 - PS417_05540 0.72 +0.5 1,247,696 - +0.2 1,247,801 - PS417_05540 0.78 +0.1 1,247,823 - +1.4 1,247,860 + PS417_05540 0.81 +0.3 1,247,860 + PS417_05540 0.81 +0.4 1,247,860 + PS417_05540 0.81 -1.1 1,247,860 + PS417_05540 0.81 +2.1 1,247,861 - PS417_05540 0.81 -2.0 1,247,861 - PS417_05540 0.81 -1.1 1,247,861 - PS417_05540 0.81 +0.9 1,247,861 - PS417_05540 0.81 +0.1 1,247,990 - PS417_05540 0.88 +0.0 1,247,990 - PS417_05540 0.88 +1.6 1,247,996 - PS417_05540 0.88 -2.2 1,248,076 + -1.9 1,248,080 - +0.8 1,248,580 + PS417_05545 0.27 -3.7 1,248,580 + PS417_05545 0.27 -2.4 1,248,581 - PS417_05545 0.27 +0.1 1,248,590 + PS417_05545 0.29 -1.2 1,248,590 - +0.5 1,248,590 - PS417_05545 0.29 -4.5 1,248,800 - PS417_05545 0.57 -1.1 1,248,800 - PS417_05545 0.57 -1.2 1,249,083 - +0.2 1,249,095 + +0.3 1,249,096 - +0.9 1,249,096 - -0.6 1,249,375 + PS417_05550 0.39 +1.3 1,249,375 + PS417_05550 0.39 -0.0 1,249,376 - -0.2 1,249,559 - PS417_05550 0.67 -2.7 1,249,600 + PS417_05550 0.73 -2.5 1,249,610 - PS417_05550 0.74 +0.0 1,249,610 - PS417_05550 0.74 -0.4 1,249,782 + -2.3 1,249,783 - +0.1 1,249,872 + PS417_05555 0.12 +0.7 1,249,872 + PS417_05555 0.12 +1.0 1,249,873 - PS417_05555 0.12 -1.8 1,250,062 - PS417_05555 0.38 -1.7
Or see this region's nucleotide sequence