Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_05425 and PS417_05430 overlap by 4 nucleotides PS417_05430 and PS417_05435 are separated by 25 nucleotides PS417_05435 and PS417_05440 are separated by 215 nucleotides
PS417_05425: PS417_05425 - histidine kinase, at 1,217,594 to 1,219,411
_05425
PS417_05430: PS417_05430 - cytochrome B6, at 1,219,408 to 1,220,376
_05430
PS417_05435: PS417_05435 - diguanylate cyclase, at 1,220,402 to 1,222,276
_05435
PS417_05440: PS417_05440 - chemotaxis protein CheY, at 1,222,492 to 1,223,793
_05440
Position (kb)
1220
1221
1222
1223 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1219.503 kb on - strand at 1219.683 kb on - strand, within PS417_05430 at 1219.713 kb on + strand, within PS417_05430 at 1219.713 kb on + strand, within PS417_05430 at 1219.714 kb on - strand, within PS417_05430 at 1219.714 kb on - strand, within PS417_05430 at 1219.714 kb on - strand, within PS417_05430 at 1219.714 kb on - strand, within PS417_05430 at 1219.757 kb on + strand, within PS417_05430 at 1219.781 kb on + strand, within PS417_05430 at 1219.812 kb on + strand, within PS417_05430 at 1219.816 kb on + strand, within PS417_05430 at 1219.816 kb on + strand, within PS417_05430 at 1219.816 kb on + strand, within PS417_05430 at 1219.817 kb on - strand, within PS417_05430 at 1219.817 kb on - strand, within PS417_05430 at 1219.865 kb on + strand, within PS417_05430 at 1219.865 kb on + strand, within PS417_05430 at 1219.865 kb on + strand, within PS417_05430 at 1219.866 kb on - strand, within PS417_05430 at 1219.866 kb on - strand, within PS417_05430 at 1219.937 kb on + strand, within PS417_05430 at 1220.008 kb on + strand, within PS417_05430 at 1220.008 kb on + strand, within PS417_05430 at 1220.601 kb on - strand, within PS417_05435 at 1220.621 kb on - strand, within PS417_05435 at 1220.919 kb on + strand, within PS417_05435 at 1220.920 kb on - strand, within PS417_05435 at 1221.303 kb on + strand, within PS417_05435 at 1221.303 kb on + strand, within PS417_05435 at 1221.318 kb on + strand, within PS417_05435 at 1221.595 kb on + strand, within PS417_05435 at 1221.599 kb on + strand, within PS417_05435 at 1221.600 kb on - strand, within PS417_05435 at 1221.638 kb on + strand, within PS417_05435 at 1221.700 kb on - strand, within PS417_05435 at 1222.243 kb on + strand at 1222.353 kb on - strand at 1222.526 kb on + strand at 1222.527 kb on - strand at 1222.571 kb on + strand at 1222.595 kb on + strand at 1222.595 kb on + strand at 1222.596 kb on - strand at 1222.674 kb on + strand, within PS417_05440 at 1222.675 kb on - strand, within PS417_05440 at 1222.724 kb on - strand, within PS417_05440 at 1222.810 kb on + strand, within PS417_05440 at 1222.811 kb on - strand, within PS417_05440 at 1223.020 kb on + strand, within PS417_05440 at 1223.021 kb on - strand, within PS417_05440 at 1223.021 kb on - strand, within PS417_05440 at 1223.021 kb on - strand, within PS417_05440 at 1223.021 kb on - strand, within PS417_05440 at 1223.021 kb on - strand, within PS417_05440 at 1223.116 kb on + strand, within PS417_05440 at 1223.117 kb on - strand at 1223.233 kb on - strand, within PS417_05440
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 1,219,503 - +0.2 1,219,683 - PS417_05430 0.28 -1.6 1,219,713 + PS417_05430 0.31 +1.6 1,219,713 + PS417_05430 0.31 -1.7 1,219,714 - PS417_05430 0.32 +1.3 1,219,714 - PS417_05430 0.32 +0.9 1,219,714 - PS417_05430 0.32 -1.3 1,219,714 - PS417_05430 0.32 -0.3 1,219,757 + PS417_05430 0.36 +1.1 1,219,781 + PS417_05430 0.38 -1.1 1,219,812 + PS417_05430 0.42 +0.2 1,219,816 + PS417_05430 0.42 -0.4 1,219,816 + PS417_05430 0.42 +0.2 1,219,816 + PS417_05430 0.42 -0.2 1,219,817 - PS417_05430 0.42 +0.6 1,219,817 - PS417_05430 0.42 -2.2 1,219,865 + PS417_05430 0.47 -1.9 1,219,865 + PS417_05430 0.47 +0.4 1,219,865 + PS417_05430 0.47 -0.1 1,219,866 - PS417_05430 0.47 -1.8 1,219,866 - PS417_05430 0.47 -0.2 1,219,937 + PS417_05430 0.55 +0.0 1,220,008 + PS417_05430 0.62 +2.0 1,220,008 + PS417_05430 0.62 -2.6 1,220,601 - PS417_05435 0.11 +0.9 1,220,621 - PS417_05435 0.12 -3.3 1,220,919 + PS417_05435 0.28 -0.0 1,220,920 - PS417_05435 0.28 +0.4 1,221,303 + PS417_05435 0.48 +0.0 1,221,303 + PS417_05435 0.48 -2.2 1,221,318 + PS417_05435 0.49 +1.1 1,221,595 + PS417_05435 0.64 -1.7 1,221,599 + PS417_05435 0.64 +3.6 1,221,600 - PS417_05435 0.64 +1.7 1,221,638 + PS417_05435 0.66 -0.2 1,221,700 - PS417_05435 0.69 +0.6 1,222,243 + +2.0 1,222,353 - +1.0 1,222,526 + +0.1 1,222,527 - +0.3 1,222,571 + +1.1 1,222,595 + -2.5 1,222,595 + +0.5 1,222,596 - -0.8 1,222,674 + PS417_05440 0.14 -1.2 1,222,675 - PS417_05440 0.14 +1.4 1,222,724 - PS417_05440 0.18 -0.1 1,222,810 + PS417_05440 0.24 -1.9 1,222,811 - PS417_05440 0.25 +0.6 1,223,020 + PS417_05440 0.41 -0.2 1,223,021 - PS417_05440 0.41 -0.3 1,223,021 - PS417_05440 0.41 +2.2 1,223,021 - PS417_05440 0.41 -0.4 1,223,021 - PS417_05440 0.41 -1.4 1,223,021 - PS417_05440 0.41 -2.1 1,223,116 + PS417_05440 0.48 -0.4 1,223,117 - +0.2 1,223,233 - PS417_05440 0.57 +2.5
Or see this region's nucleotide sequence