Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_03275 and PS417_03280 are separated by 61 nucleotides PS417_03280 and PS417_03285 overlap by 4 nucleotides PS417_03285 and PS417_03290 overlap by 11 nucleotides
PS417_03275: PS417_03275 - mammalian cell entry protein, at 749,018 to 751,321
_03275
PS417_03280: PS417_03280 - chromosome partitioning protein ParA, at 751,383 to 754,223
_03280
PS417_03285: PS417_03285 - chromosome partitioning protein, at 754,220 to 754,927
_03285
PS417_03290: PS417_03290 - hypothetical protein, at 754,917 to 756,194
_03290
Position (kb) 751
752
753
754
755 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 750.630 kb on + strand, within PS417_03275 at 750.656 kb on - strand, within PS417_03275 at 750.713 kb on - strand, within PS417_03275 at 750.796 kb on + strand, within PS417_03275 at 750.824 kb on + strand, within PS417_03275 at 750.825 kb on - strand, within PS417_03275 at 750.980 kb on + strand, within PS417_03275 at 751.070 kb on + strand, within PS417_03275 at 751.070 kb on + strand, within PS417_03275 at 751.071 kb on - strand, within PS417_03275 at 751.457 kb on - strand at 751.457 kb on - strand at 751.480 kb on + strand at 751.480 kb on + strand at 751.480 kb on + strand at 751.481 kb on - strand at 751.639 kb on + strand at 751.643 kb on - strand at 751.643 kb on - strand at 751.696 kb on + strand, within PS417_03280 at 751.696 kb on + strand, within PS417_03280 at 751.696 kb on + strand, within PS417_03280 at 751.697 kb on - strand, within PS417_03280 at 751.697 kb on - strand, within PS417_03280 at 751.697 kb on - strand, within PS417_03280 at 751.865 kb on - strand, within PS417_03280 at 751.976 kb on + strand, within PS417_03280 at 751.977 kb on - strand, within PS417_03280 at 752.106 kb on - strand, within PS417_03280 at 752.189 kb on - strand, within PS417_03280 at 752.189 kb on - strand, within PS417_03280 at 752.262 kb on + strand, within PS417_03280 at 752.272 kb on + strand at 752.272 kb on + strand, within PS417_03280 at 752.272 kb on + strand, within PS417_03280 at 752.273 kb on - strand, within PS417_03280 at 752.273 kb on - strand, within PS417_03280 at 752.327 kb on - strand at 752.665 kb on + strand, within PS417_03280 at 752.666 kb on - strand, within PS417_03280 at 752.841 kb on + strand, within PS417_03280 at 753.019 kb on + strand, within PS417_03280 at 753.019 kb on + strand, within PS417_03280 at 753.020 kb on - strand, within PS417_03280 at 753.020 kb on - strand, within PS417_03280 at 753.020 kb on - strand, within PS417_03280 at 753.184 kb on - strand, within PS417_03280 at 753.514 kb on + strand, within PS417_03280 at 753.514 kb on + strand, within PS417_03280 at 753.514 kb on + strand, within PS417_03280 at 753.534 kb on + strand, within PS417_03280 at 753.535 kb on - strand, within PS417_03280 at 753.535 kb on - strand, within PS417_03280 at 753.746 kb on + strand, within PS417_03280 at 753.794 kb on - strand, within PS417_03280 at 753.988 kb on + strand at 753.988 kb on + strand at 753.988 kb on + strand at 753.989 kb on - strand at 754.036 kb on + strand at 754.036 kb on + strand at 754.106 kb on + strand at 754.126 kb on + strand at 754.126 kb on + strand at 754.127 kb on - strand at 754.378 kb on + strand, within PS417_03285 at 754.570 kb on - strand, within PS417_03285 at 754.743 kb on + strand, within PS417_03285 at 754.746 kb on + strand, within PS417_03285 at 754.747 kb on - strand, within PS417_03285 at 754.834 kb on - strand, within PS417_03285 at 754.934 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 750,630 + PS417_03275 0.70 +0.9 750,656 - PS417_03275 0.71 -1.4 750,713 - PS417_03275 0.74 +1.7 750,796 + PS417_03275 0.77 -1.0 750,824 + PS417_03275 0.78 -0.5 750,825 - PS417_03275 0.78 +0.3 750,980 + PS417_03275 0.85 +1.3 751,070 + PS417_03275 0.89 -0.3 751,070 + PS417_03275 0.89 +1.0 751,071 - PS417_03275 0.89 +0.0 751,457 - +1.4 751,457 - -2.6 751,480 + -1.5 751,480 + +0.0 751,480 + +0.6 751,481 - -0.9 751,639 + -0.1 751,643 - -1.6 751,643 - -2.0 751,696 + PS417_03280 0.11 -0.7 751,696 + PS417_03280 0.11 -1.0 751,696 + PS417_03280 0.11 +0.5 751,697 - PS417_03280 0.11 -0.1 751,697 - PS417_03280 0.11 -0.7 751,697 - PS417_03280 0.11 +0.0 751,865 - PS417_03280 0.17 -2.9 751,976 + PS417_03280 0.21 -1.6 751,977 - PS417_03280 0.21 -2.4 752,106 - PS417_03280 0.25 -0.0 752,189 - PS417_03280 0.28 -0.5 752,189 - PS417_03280 0.28 +0.3 752,262 + PS417_03280 0.31 +0.2 752,272 + +0.0 752,272 + PS417_03280 0.31 +0.4 752,272 + PS417_03280 0.31 -0.5 752,273 - PS417_03280 0.31 +0.3 752,273 - PS417_03280 0.31 -0.8 752,327 - -0.4 752,665 + PS417_03280 0.45 -1.2 752,666 - PS417_03280 0.45 +0.0 752,841 + PS417_03280 0.51 -0.2 753,019 + PS417_03280 0.58 +0.1 753,019 + PS417_03280 0.58 -2.8 753,020 - PS417_03280 0.58 +0.6 753,020 - PS417_03280 0.58 -1.8 753,020 - PS417_03280 0.58 -1.7 753,184 - PS417_03280 0.63 +0.1 753,514 + PS417_03280 0.75 -0.2 753,514 + PS417_03280 0.75 -0.4 753,514 + PS417_03280 0.75 -0.5 753,534 + PS417_03280 0.76 -0.2 753,535 - PS417_03280 0.76 -0.4 753,535 - PS417_03280 0.76 -0.1 753,746 + PS417_03280 0.83 -0.8 753,794 - PS417_03280 0.85 -1.4 753,988 + +0.8 753,988 + -0.0 753,988 + +0.0 753,989 - +0.0 754,036 + +0.2 754,036 + -0.4 754,106 + -0.1 754,126 + -0.7 754,126 + +0.6 754,127 - +0.4 754,378 + PS417_03285 0.22 -1.4 754,570 - PS417_03285 0.49 -0.1 754,743 + PS417_03285 0.74 -1.1 754,746 + PS417_03285 0.74 -1.6 754,747 - PS417_03285 0.74 +0.8 754,834 - PS417_03285 0.87 +0.2 754,934 + -1.5
Or see this region's nucleotide sequence