Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_25600 and PS417_25605 are separated by 45 nucleotides PS417_25605 and PS417_25610 are separated by 161 nucleotides PS417_25610 and PS417_25615 are separated by 26 nucleotides PS417_25615 and PS417_25620 are separated by 24 nucleotides PS417_25620 and PS417_25625 are separated by 143 nucleotides PS417_25625 and PS417_25630 are separated by 7 nucleotides PS417_25630 and PS417_25635 overlap by 11 nucleotides
PS417_25600: PS417_25600 - preprotein translocase subunit SecE, at 5,534,965 to 5,535,333
_25600
PS417_25605: PS417_25605 - tRNA-Trp, at 5,535,379 to 5,535,454
_25605
PS417_25610: PS417_25610 - tRNA-Thr, at 5,535,616 to 5,535,691
_25610
PS417_25615: PS417_25615 - tRNA-Gly, at 5,535,718 to 5,535,791
_25615
PS417_25620: PS417_25620 - tRNA-Tyr, at 5,535,816 to 5,535,900
_25620
PS417_25625: PS417_25625 - hypothetical protein, at 5,536,044 to 5,536,490
_25625
PS417_25630: PS417_25630 - pantothenate kinase, at 5,536,498 to 5,537,247
_25630
PS417_25635: PS417_25635 - biotin--protein ligase, at 5,537,237 to 5,538,196
_25635
Position (kb)
5536
5537 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5535.518 kb on - strand at 5535.551 kb on - strand at 5535.551 kb on - strand at 5535.593 kb on - strand at 5535.593 kb on - strand at 5535.601 kb on - strand at 5535.615 kb on - strand at 5536.037 kb on - strand at 5536.037 kb on - strand at 5536.037 kb on - strand at 5536.037 kb on - strand at 5536.037 kb on - strand at 5536.078 kb on - strand at 5536.190 kb on + strand, within PS417_25625 at 5536.191 kb on - strand, within PS417_25625 at 5536.215 kb on + strand, within PS417_25625 at 5536.215 kb on + strand, within PS417_25625 at 5536.216 kb on - strand, within PS417_25625 at 5536.216 kb on - strand, within PS417_25625 at 5536.216 kb on - strand, within PS417_25625 at 5536.217 kb on + strand, within PS417_25625 at 5536.218 kb on - strand, within PS417_25625 at 5536.282 kb on + strand, within PS417_25625 at 5536.282 kb on + strand, within PS417_25625 at 5536.283 kb on - strand, within PS417_25625 at 5536.283 kb on - strand, within PS417_25625 at 5536.283 kb on - strand, within PS417_25625 at 5536.292 kb on + strand, within PS417_25625 at 5536.384 kb on + strand, within PS417_25625 at 5536.385 kb on - strand, within PS417_25625 at 5536.385 kb on - strand, within PS417_25625 at 5536.385 kb on - strand, within PS417_25625 at 5536.444 kb on + strand, within PS417_25625 at 5536.445 kb on - strand, within PS417_25625 at 5536.445 kb on - strand, within PS417_25625 at 5536.447 kb on + strand at 5536.447 kb on + strand at 5536.447 kb on + strand at 5536.448 kb on - strand at 5536.448 kb on - strand at 5536.490 kb on + strand at 5536.491 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 5,535,518 - -2.1 5,535,551 - -1.5 5,535,551 - -0.0 5,535,593 - +2.3 5,535,593 - +0.1 5,535,601 - -1.6 5,535,615 - +0.5 5,536,037 - -0.9 5,536,037 - -1.3 5,536,037 - -2.8 5,536,037 - -0.3 5,536,037 - -0.1 5,536,078 - +0.0 5,536,190 + PS417_25625 0.33 -0.3 5,536,191 - PS417_25625 0.33 -2.0 5,536,215 + PS417_25625 0.38 -1.9 5,536,215 + PS417_25625 0.38 -2.8 5,536,216 - PS417_25625 0.38 -1.0 5,536,216 - PS417_25625 0.38 -1.0 5,536,216 - PS417_25625 0.38 -1.1 5,536,217 + PS417_25625 0.39 -0.8 5,536,218 - PS417_25625 0.39 -0.7 5,536,282 + PS417_25625 0.53 +0.8 5,536,282 + PS417_25625 0.53 +1.3 5,536,283 - PS417_25625 0.53 -2.6 5,536,283 - PS417_25625 0.53 -0.6 5,536,283 - PS417_25625 0.53 -1.1 5,536,292 + PS417_25625 0.55 -1.5 5,536,384 + PS417_25625 0.76 -0.4 5,536,385 - PS417_25625 0.76 -1.0 5,536,385 - PS417_25625 0.76 -0.6 5,536,385 - PS417_25625 0.76 -0.2 5,536,444 + PS417_25625 0.89 +0.8 5,536,445 - PS417_25625 0.90 -0.5 5,536,445 - PS417_25625 0.90 +0.9 5,536,447 + -0.0 5,536,447 + +0.2 5,536,447 + -0.4 5,536,448 - -0.1 5,536,448 - -1.0 5,536,490 + -0.6 5,536,491 - -0.4
Or see this region's nucleotide sequence