Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_24515 and PS417_24520 are separated by 224 nucleotides PS417_24520 and PS417_24525 are separated by 25 nucleotides PS417_24525 and PS417_24530 are separated by 117 nucleotides
PS417_24515: PS417_24515 - acyl carrier protein, at 5,320,551 to 5,320,727
_24515
PS417_24520: PS417_24520 - DNA polymerase III subunit epsilon, at 5,320,952 to 5,321,563
_24520
PS417_24525: PS417_24525 - nuclease, at 5,321,589 to 5,322,065
_24525
PS417_24530: PS417_24530 - ABC transporter substrate-binding protein, at 5,322,183 to 5,322,914
_24530
Position (kb)
5321
5322
5323 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 5320.765 kb on - strand at 5320.821 kb on + strand at 5320.822 kb on - strand at 5320.825 kb on + strand at 5320.826 kb on - strand at 5320.826 kb on - strand at 5320.869 kb on + strand at 5320.869 kb on + strand at 5320.870 kb on - strand at 5320.871 kb on + strand at 5320.871 kb on + strand at 5320.871 kb on + strand at 5320.872 kb on - strand at 5320.872 kb on - strand at 5321.151 kb on + strand, within PS417_24520 at 5321.151 kb on + strand, within PS417_24520 at 5321.265 kb on + strand, within PS417_24520 at 5321.265 kb on + strand, within PS417_24520 at 5321.265 kb on + strand, within PS417_24520 at 5321.266 kb on - strand, within PS417_24520 at 5321.554 kb on + strand at 5321.554 kb on + strand at 5321.554 kb on + strand at 5321.555 kb on - strand at 5321.555 kb on - strand at 5321.555 kb on - strand at 5321.555 kb on - strand at 5321.590 kb on + strand at 5321.595 kb on - strand at 5321.674 kb on + strand, within PS417_24525 at 5321.845 kb on + strand, within PS417_24525 at 5321.846 kb on - strand, within PS417_24525 at 5321.929 kb on + strand, within PS417_24525 at 5321.936 kb on - strand, within PS417_24525 at 5322.022 kb on + strand at 5322.022 kb on + strand at 5322.023 kb on - strand at 5322.026 kb on - strand at 5322.496 kb on + strand, within PS417_24530 at 5322.497 kb on - strand, within PS417_24530 at 5322.497 kb on - strand, within PS417_24530 at 5322.717 kb on - strand, within PS417_24530 at 5322.775 kb on + strand, within PS417_24530 at 5322.775 kb on + strand, within PS417_24530 at 5322.775 kb on + strand, within PS417_24530 at 5322.776 kb on - strand, within PS417_24530 at 5322.776 kb on - strand, within PS417_24530 at 5322.776 kb on - strand, within PS417_24530 at 5322.974 kb on + strand at 5322.974 kb on + strand at 5322.975 kb on - strand at 5322.975 kb on - strand at 5323.022 kb on + strand at 5323.023 kb on - strand at 5323.023 kb on - strand at 5323.032 kb on + strand at 5323.033 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 5,320,765 - +0.2 5,320,821 + -0.9 5,320,822 - -0.7 5,320,825 + +1.0 5,320,826 - -0.4 5,320,826 - +0.3 5,320,869 + -0.1 5,320,869 + +1.4 5,320,870 - -0.7 5,320,871 + -0.1 5,320,871 + -2.3 5,320,871 + +0.5 5,320,872 - -1.0 5,320,872 - +0.0 5,321,151 + PS417_24520 0.33 -2.0 5,321,151 + PS417_24520 0.33 -0.5 5,321,265 + PS417_24520 0.51 +0.7 5,321,265 + PS417_24520 0.51 +2.3 5,321,265 + PS417_24520 0.51 +0.2 5,321,266 - PS417_24520 0.51 +0.2 5,321,554 + +0.8 5,321,554 + -2.3 5,321,554 + -0.6 5,321,555 - -0.9 5,321,555 - -0.7 5,321,555 - +0.3 5,321,555 - -1.5 5,321,590 + -0.2 5,321,595 - +0.7 5,321,674 + PS417_24525 0.18 -1.3 5,321,845 + PS417_24525 0.54 -1.3 5,321,846 - PS417_24525 0.54 -1.5 5,321,929 + PS417_24525 0.71 -1.9 5,321,936 - PS417_24525 0.73 -1.3 5,322,022 + -1.7 5,322,022 + -0.4 5,322,023 - -1.0 5,322,026 - -1.5 5,322,496 + PS417_24530 0.43 -0.5 5,322,497 - PS417_24530 0.43 +1.4 5,322,497 - PS417_24530 0.43 -0.7 5,322,717 - PS417_24530 0.73 -0.7 5,322,775 + PS417_24530 0.81 -0.6 5,322,775 + PS417_24530 0.81 -1.7 5,322,775 + PS417_24530 0.81 +1.0 5,322,776 - PS417_24530 0.81 +3.5 5,322,776 - PS417_24530 0.81 +1.1 5,322,776 - PS417_24530 0.81 -0.9 5,322,974 + +0.8 5,322,974 + -0.8 5,322,975 - +0.3 5,322,975 - +0.7 5,323,022 + -0.1 5,323,023 - +0.1 5,323,023 - +0.2 5,323,032 + +0.8 5,323,033 - -0.8
Or see this region's nucleotide sequence