Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_02360 and PS417_02365 overlap by 32 nucleotides PS417_02365 and PS417_02370 are separated by 193 nucleotides PS417_02370 and PS417_02375 overlap by 10 nucleotides PS417_02375 and PS417_02380 are separated by 97 nucleotides
PS417_02360: PS417_02360 - nitrate reductase, at 540,437 to 541,726
_02360
PS417_02365: PS417_02365 - hypothetical protein, at 541,695 to 542,441
_02365
PS417_02370: PS417_02370 - ADP-ribose pyrophosphatase, at 542,635 to 543,252
_02370
PS417_02375: PS417_02375 - hypothetical protein, at 543,243 to 543,692
_02375
PS417_02380: PS417_02380 - serine/threonine protein phosphatase, at 543,790 to 544,605
_02380
Position (kb) 542
543
544 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 541.780 kb on + strand, within PS417_02365 at 541.781 kb on - strand, within PS417_02365 at 542.008 kb on + strand, within PS417_02365 at 542.060 kb on - strand, within PS417_02365 at 542.103 kb on + strand, within PS417_02365 at 542.363 kb on - strand, within PS417_02365 at 542.363 kb on - strand, within PS417_02365 at 542.463 kb on - strand at 542.516 kb on - strand at 542.635 kb on - strand at 542.707 kb on + strand, within PS417_02370 at 542.708 kb on - strand, within PS417_02370 at 543.220 kb on - strand at 543.251 kb on - strand at 543.312 kb on + strand, within PS417_02375 at 543.312 kb on + strand, within PS417_02375 at 543.313 kb on - strand, within PS417_02375 at 543.435 kb on + strand, within PS417_02375 at 543.511 kb on - strand, within PS417_02375 at 543.511 kb on - strand, within PS417_02375 at 543.573 kb on + strand, within PS417_02375 at 543.573 kb on + strand, within PS417_02375 at 543.574 kb on - strand, within PS417_02375 at 543.690 kb on + strand at 543.715 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 541,780 + PS417_02365 0.11 +0.5 541,781 - PS417_02365 0.12 -1.5 542,008 + PS417_02365 0.42 +0.1 542,060 - PS417_02365 0.49 -2.9 542,103 + PS417_02365 0.55 -0.7 542,363 - PS417_02365 0.89 +3.8 542,363 - PS417_02365 0.89 -0.8 542,463 - -0.4 542,516 - +3.0 542,635 - -0.2 542,707 + PS417_02370 0.12 -2.4 542,708 - PS417_02370 0.12 -0.9 543,220 - +0.4 543,251 - +0.8 543,312 + PS417_02375 0.15 +1.7 543,312 + PS417_02375 0.15 +0.3 543,313 - PS417_02375 0.16 -2.1 543,435 + PS417_02375 0.43 +1.4 543,511 - PS417_02375 0.60 -0.0 543,511 - PS417_02375 0.60 +0.4 543,573 + PS417_02375 0.73 -0.3 543,573 + PS417_02375 0.73 -0.9 543,574 - PS417_02375 0.74 -0.1 543,690 + -0.1 543,715 - -1.1
Or see this region's nucleotide sequence