Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_22170 and PS417_22175 are separated by 5 nucleotides PS417_22175 and PS417_22180 are separated by 178 nucleotides PS417_22180 and PS417_22185 are separated by 139 nucleotides PS417_22185 and PS417_22190 are separated by 207 nucleotides
PS417_22170: PS417_22170 - acetyltransferase, at 4,803,336 to 4,803,737
_22170
PS417_22175: PS417_22175 - amino acid transporter LysE, at 4,803,743 to 4,804,342
_22175
PS417_22180: PS417_22180 - superoxide dismutase, at 4,804,521 to 4,805,117
_22180
PS417_22185: PS417_22185 - hypothetical protein, at 4,805,257 to 4,805,442
_22185
PS417_22190: PS417_22190 - diguanylate cyclase, at 4,805,650 to 4,807,701
_22190
Position (kb)
4804
4805
4806 Strain fitness (log2 ratio)
-1
0
1
2
3
4
5 at 4803.654 kb on + strand at 4803.706 kb on + strand at 4803.706 kb on + strand at 4803.707 kb on - strand at 4803.736 kb on - strand at 4803.742 kb on + strand at 4803.743 kb on - strand at 4803.820 kb on - strand, within PS417_22175 at 4804.178 kb on + strand, within PS417_22175 at 4804.414 kb on + strand at 4805.116 kb on - strand at 4805.415 kb on + strand, within PS417_22185 at 4805.483 kb on + strand at 4805.484 kb on - strand at 4805.522 kb on - strand at 4805.614 kb on - strand at 4805.761 kb on + strand at 4806.056 kb on - strand, within PS417_22190 at 4806.094 kb on + strand, within PS417_22190 at 4806.094 kb on + strand, within PS417_22190 at 4806.094 kb on + strand, within PS417_22190 at 4806.095 kb on - strand, within PS417_22190
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 4,803,654 + +3.3 4,803,706 + -0.3 4,803,706 + -0.6 4,803,707 - -0.8 4,803,736 - -0.1 4,803,742 + +0.1 4,803,743 - -0.3 4,803,820 - PS417_22175 0.13 -0.2 4,804,178 + PS417_22175 0.72 +0.7 4,804,414 + -1.0 4,805,116 - -0.6 4,805,415 + PS417_22185 0.85 +0.8 4,805,483 + -1.5 4,805,484 - +5.0 4,805,522 - +0.7 4,805,614 - +0.0 4,805,761 + +2.1 4,806,056 - PS417_22190 0.20 +1.5 4,806,094 + PS417_22190 0.22 +2.4 4,806,094 + PS417_22190 0.22 -1.2 4,806,094 + PS417_22190 0.22 +3.8 4,806,095 - PS417_22190 0.22 +0.7
Or see this region's nucleotide sequence