Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_22080 and PS417_22085 are separated by 55 nucleotides PS417_22085 and PS417_22090 are separated by 205 nucleotides PS417_22090 and PS417_22095 are separated by 12 nucleotides PS417_22095 and PS417_22100 are separated by 93 nucleotides PS417_22100 and PS417_22105 are separated by 17 nucleotides
PS417_22080: PS417_22080 - 3-hydroxybutyryl-CoA dehydratase, at 4,785,308 to 4,785,778
_22080
PS417_22085: PS417_22085 - aldehyde dehydrogenase, at 4,785,834 to 4,786,301
_22085
PS417_22090: PS417_22090 - hypothetical protein, at 4,786,507 to 4,786,743
_22090
PS417_22095: PS417_22095 - class V aminotransferase, at 4,786,756 to 4,787,931
_22095
PS417_22100: PS417_22100 - keto-deoxy-phosphogluconate aldolase, at 4,788,025 to 4,788,690
_22100
PS417_22105: PS417_22105 - 6-phosphogluconolactonase, at 4,788,708 to 4,789,421
_22105
Position (kb) 4786
4787
4788 Strain fitness (log2 ratio) -1
0
1
2
3 at 4785.857 kb on + strand at 4785.892 kb on - strand, within PS417_22085 at 4785.901 kb on + strand, within PS417_22085 at 4785.901 kb on + strand, within PS417_22085 at 4786.424 kb on - strand at 4786.562 kb on - strand, within PS417_22090 at 4786.562 kb on - strand, within PS417_22090 at 4786.755 kb on + strand at 4786.755 kb on + strand at 4786.756 kb on - strand at 4786.756 kb on - strand at 4786.902 kb on + strand, within PS417_22095 at 4786.928 kb on + strand, within PS417_22095 at 4786.928 kb on + strand, within PS417_22095 at 4787.059 kb on + strand, within PS417_22095 at 4787.060 kb on - strand, within PS417_22095 at 4787.060 kb on - strand, within PS417_22095 at 4787.088 kb on - strand, within PS417_22095 at 4787.215 kb on + strand, within PS417_22095 at 4787.343 kb on - strand, within PS417_22095 at 4787.343 kb on - strand, within PS417_22095 at 4787.464 kb on + strand, within PS417_22095 at 4787.545 kb on - strand, within PS417_22095 at 4787.545 kb on - strand, within PS417_22095 at 4787.545 kb on - strand, within PS417_22095 at 4787.545 kb on - strand, within PS417_22095 at 4787.545 kb on - strand, within PS417_22095 at 4787.651 kb on - strand, within PS417_22095 at 4787.651 kb on - strand, within PS417_22095 at 4787.669 kb on + strand, within PS417_22095 at 4787.729 kb on - strand, within PS417_22095 at 4787.930 kb on - strand at 4787.930 kb on - strand at 4787.961 kb on - strand at 4788.015 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 4,785,857 + -0.5 4,785,892 - PS417_22085 0.12 +0.9 4,785,901 + PS417_22085 0.14 +0.6 4,785,901 + PS417_22085 0.14 -0.4 4,786,424 - +0.4 4,786,562 - PS417_22090 0.23 -1.2 4,786,562 - PS417_22090 0.23 +0.9 4,786,755 + +0.1 4,786,755 + +0.5 4,786,756 - -0.7 4,786,756 - -0.1 4,786,902 + PS417_22095 0.12 +1.4 4,786,928 + PS417_22095 0.15 -0.9 4,786,928 + PS417_22095 0.15 +1.8 4,787,059 + PS417_22095 0.26 +0.3 4,787,060 - PS417_22095 0.26 +2.7 4,787,060 - PS417_22095 0.26 +0.1 4,787,088 - PS417_22095 0.28 -0.1 4,787,215 + PS417_22095 0.39 +0.4 4,787,343 - PS417_22095 0.50 +3.3 4,787,343 - PS417_22095 0.50 +0.8 4,787,464 + PS417_22095 0.60 +0.2 4,787,545 - PS417_22095 0.67 +0.7 4,787,545 - PS417_22095 0.67 +0.9 4,787,545 - PS417_22095 0.67 -0.5 4,787,545 - PS417_22095 0.67 +0.7 4,787,545 - PS417_22095 0.67 -0.4 4,787,651 - PS417_22095 0.76 +0.0 4,787,651 - PS417_22095 0.76 -0.3 4,787,669 + PS417_22095 0.78 -0.3 4,787,729 - PS417_22095 0.83 +0.8 4,787,930 - -0.0 4,787,930 - +0.3 4,787,961 - -0.0 4,788,015 + +0.2
Or see this region's nucleotide sequence