Experiment: R2A with Polymyxin B sulfate 0.003 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_21950 and PS417_21955 are separated by 34 nucleotides PS417_21955 and PS417_21960 are separated by 42 nucleotides PS417_21960 and PS417_21965 are separated by 146 nucleotides PS417_21965 and PS417_21970 are separated by 301 nucleotides PS417_21970 and PS417_21975 are separated by 195 nucleotides
PS417_21950: PS417_21950 - polyketide cyclase, at 4,761,953 to 4,762,429
_21950
PS417_21955: PS417_21955 - dehydrogenase, at 4,762,464 to 4,762,808
_21955
PS417_21960: PS417_21960 - ketosteroid isomerase, at 4,762,851 to 4,763,270
_21960
PS417_21965: PS417_21965 - GNAT family acetyltransferase, at 4,763,417 to 4,763,935
_21965
PS417_21970: PS417_21970 - AraC family transcriptional regulator, at 4,764,237 to 4,764,719
_21970
PS417_21975: PS417_21975 - glutamate-pyruvate aminotransferase, at 4,764,915 to 4,766,126
_21975
Position (kb)
4763
4764 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4762.424 kb on - strand at 4762.451 kb on - strand at 4762.451 kb on - strand at 4762.451 kb on - strand at 4762.573 kb on + strand, within PS417_21955 at 4762.573 kb on + strand, within PS417_21955 at 4762.723 kb on + strand, within PS417_21955 at 4762.801 kb on + strand at 4762.801 kb on + strand at 4762.838 kb on + strand at 4762.838 kb on + strand at 4762.839 kb on - strand at 4762.839 kb on - strand at 4763.395 kb on + strand at 4763.395 kb on + strand at 4763.396 kb on - strand at 4763.396 kb on - strand at 4763.823 kb on - strand, within PS417_21965 at 4763.858 kb on + strand, within PS417_21965 at 4763.858 kb on + strand, within PS417_21965 at 4763.858 kb on + strand, within PS417_21965 at 4763.859 kb on - strand, within PS417_21965 at 4763.859 kb on - strand, within PS417_21965 at 4763.859 kb on - strand, within PS417_21965 at 4763.859 kb on - strand, within PS417_21965 at 4763.859 kb on - strand, within PS417_21965 at 4763.859 kb on - strand, within PS417_21965 at 4763.859 kb on - strand, within PS417_21965 at 4763.860 kb on + strand, within PS417_21965 at 4763.861 kb on - strand, within PS417_21965 at 4763.861 kb on - strand, within PS417_21965 at 4763.861 kb on - strand, within PS417_21965 at 4763.861 kb on - strand, within PS417_21965 at 4763.861 kb on - strand, within PS417_21965 at 4763.934 kb on - strand at 4763.934 kb on - strand at 4763.938 kb on + strand at 4763.944 kb on + strand at 4763.975 kb on - strand at 4764.012 kb on + strand at 4764.427 kb on + strand, within PS417_21970 at 4764.428 kb on - strand, within PS417_21970 at 4764.737 kb on + strand at 4764.737 kb on + strand at 4764.738 kb on - strand at 4764.738 kb on - strand at 4764.762 kb on + strand at 4764.764 kb on + strand at 4764.765 kb on - strand at 4764.837 kb on + strand at 4764.837 kb on + strand at 4764.897 kb on - strand at 4764.897 kb on - strand at 4764.897 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.003 mg/ml remove 4,762,424 - -0.1 4,762,451 - -0.1 4,762,451 - -0.9 4,762,451 - -2.7 4,762,573 + PS417_21955 0.32 -0.3 4,762,573 + PS417_21955 0.32 +0.9 4,762,723 + PS417_21955 0.75 -0.2 4,762,801 + -2.2 4,762,801 + -0.2 4,762,838 + +1.3 4,762,838 + +0.0 4,762,839 - +0.9 4,762,839 - -2.6 4,763,395 + -0.6 4,763,395 + +0.7 4,763,396 - -0.2 4,763,396 - +0.2 4,763,823 - PS417_21965 0.78 -0.2 4,763,858 + PS417_21965 0.85 +2.8 4,763,858 + PS417_21965 0.85 -1.4 4,763,858 + PS417_21965 0.85 -0.3 4,763,859 - PS417_21965 0.85 -0.6 4,763,859 - PS417_21965 0.85 -1.9 4,763,859 - PS417_21965 0.85 -0.9 4,763,859 - PS417_21965 0.85 -1.4 4,763,859 - PS417_21965 0.85 -0.4 4,763,859 - PS417_21965 0.85 -1.9 4,763,859 - PS417_21965 0.85 +0.1 4,763,860 + PS417_21965 0.85 +0.0 4,763,861 - PS417_21965 0.86 +0.2 4,763,861 - PS417_21965 0.86 +0.8 4,763,861 - PS417_21965 0.86 -0.2 4,763,861 - PS417_21965 0.86 -0.0 4,763,861 - PS417_21965 0.86 -0.0 4,763,934 - +0.2 4,763,934 - +1.0 4,763,938 + -0.6 4,763,944 + -0.5 4,763,975 - +1.1 4,764,012 + -0.0 4,764,427 + PS417_21970 0.39 +0.1 4,764,428 - PS417_21970 0.40 -2.7 4,764,737 + -1.1 4,764,737 + -1.5 4,764,738 - +0.5 4,764,738 - -0.2 4,764,762 + -0.6 4,764,764 + -1.9 4,764,765 - -0.6 4,764,837 + -0.6 4,764,837 + -0.0 4,764,897 - +0.7 4,764,897 - +0.1 4,764,897 - -0.9
Or see this region's nucleotide sequence